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Authors
Advisor(s)
Abstract(s)
Carnaroli is a high quality and priced variety, being considered as one of the finest Italian rice varieties due to its sensorial and rheological properties and, thus being a potential adulteration target. The present work aimed at exploiting polymorphisms in the Alk (A/G and GC/TT in exon 8) and Waxy ((CT)n and G/T in intron 1) genes by HRM analysis to differentiate Carnaroli rice from closely related varieties. The HRM method targeting the Alk gene did not allow gathering the Carnaroli subgroup genotypes in the same cluster. The HRM approach targeting
Waxy gene successfully discriminated the varieties sold as Carnaroli from all the others with high level of
confidence (>98%), which corroborated sequencing data. Its applicability to commercial rice samples was
successful. Therefore, the proposed new HRM method can be considered a simple, specific, high-throughput and
cost-effective tool for the authentication of Carnaroli rice, contributing to valorise such premium variety.
Description
Keywords
Oryza sativa DNA markers High resolution melting analysis Variety differentiation Authenticity Italian rice varieties
Citation
Grazina, Liliana; Costa, Joana; Amaral, Joana S.; Garino, Cristiano; Arlorio, Marco; Mafra, I. (2022). Authentication of carnaroli rice by HRM analysis targeting nucleotide polymorphisms in the Alk and Waxy genes. Food Control. ISSN 0956-7135. 135, p. 1-10
Publisher
Elsevier