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Effects of Tryptophan and Physical Exercise on the Modulation of Mechanical Hypersensitivity in a Fibromyalgia-like Model in Female Rats

2024Journal article Open access
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dc.contributor.authorRezende, Rafael Marins
dc.contributor.authorCoimbra, Roney Santos
dc.contributor.authorKohlhoff, Markus
dc.contributor.authorFavarato, Lukiya Silva Campos
dc.contributor.authorMartino, Hércia Stampini Duarte
dc.contributor.authorLeite, Luciano Bernardes
dc.contributor.authorSoares, Leôncio Lopes
dc.contributor.authorEncarnação, Samuel
dc.contributor.authorForte, Pedro
dc.contributor.authorMonteiro, A.M.
dc.contributor.authorPeluzio, Maria do Carmo Gouveia
dc.contributor.authorNatali, Antônio José
dc.date.accessioned2024-10-28T15:47:29Z
dc.date.available2024-10-28T15:47:29Z
dc.date.issued2024
dc.description.abstractThough the mechanisms are not fully understood, tryptophan (Trp) and physical exercise seem to regulate mechanical hypersensitivity in fibromyalgia. Here, we tested the impact of Trp supplementation and continuous low-intensity aerobic exercise on the modulation of mechanical hypersensitivity in a fibromyalgia-like model induced by acid saline in female rats. Twelve-month-old femaleWistar rats were randomly divided into groups: [control (n = 6); acid saline (n = 6); acid saline + exercise (n = 6); acid saline + Trp (n = 6); and acid saline + exercise + Trp (n = 6)]. Hypersensitivity was caused using two intramuscular jabs of acid saline (20 μL; pH 4.0; right gastrocnemius), 3 days apart. The tryptophan-supplemented diet contained 7.6 g/hg of Trp. The three-week exercise consisted of progressive (30–45 min) treadmill running at 50 to 60% intensity, five times (Monday to Friday) per week. We found that acid saline induced contralateral mechanical hypersensitivity without changing the levels of Trp, serotonin (5-HT), and kynurenine (KYN) in the brain. Hypersensitivity was reduced by exercise (~150%), Trp (~67%), and its combination (~160%). The Trp supplementation increased the levels of Trp and KYN in the brain, and the activity of indoleamine 2,3-dioxygenase (IDO), and decreased the ratio 5-HT:KYN. Exercise did not impact the assessed metabolites. Combining the treatments reduced neither hypersensitivity nor the levels of serotonin and Trp in the brain. In conclusion, mechanical hypersensitivity induced by acid saline in a fibromyalgia-like model in female rats is modulated by Trp supplementation, which increases IDO activity and leads to improved Trp metabolism via the KYN pathway. In contrast, physical exercise does not affect mechanical hypersensitivity through brain Trp metabolism via either the KYN or serotonin pathways. Because this is a short study, generalizing its findings warrants caution.pt_PT
dc.description.sponsorshipThis study was financed partially by the Higher Education Personnel Improvement Coordination (CAPES-Brazil; Finance Code: PROEX/683/2018) and the Research Support Foundation of the State of Minas Gerais (FAPEMIG-Brazil; Grant number: CDS-APQ-01635-15). AJ Natali, MCG Peluzio and HSD Martino are thankful to the National Council for Scientific and Technological Development (CNPq-Brasil) for the fellowship.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRezende, Rafael Marins; Coimbra, Roney Santos; Kohlhoff, Markus; Favarato, Lukiya Silva Campos; Martino, Hércia Stampini Duarte; Leite, Luciano Bernardes; Soares, Leoncio Lopes; Encarnação, Samuel; Forte, Pedro; Monteiro, António Miguel de Barros; Peluzio, Maria do Carmo Gouveia; Natali, Antônio José (2024). Effects of Tryptophan and Physical Exercise on the Modulation of Mechanical Hypersensitivity in a Fibromyalgia-like Model in Female Rats. Cells. ISSN 2073-4409. 13:19, p. 1-11pt_PT
dc.identifier.doi10.3390/cells13191647pt_PT
dc.identifier.issn2073-4409
dc.identifier.urihttp://hdl.handle.net/10198/30489
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectFibromyalgiapt_PT
dc.subjectTreadmill runningpt_PT
dc.subjectBrainpt_PT
dc.subjectHypersensitivitypt_PT
dc.subjectTryptophan
dc.subjectSerotonin
dc.subjectKynurenine
dc.subjectIndolamine
dc.titleEffects of Tryptophan and Physical Exercise on the Modulation of Mechanical Hypersensitivity in a Fibromyalgia-like Model in Female Ratspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage11pt_PT
oaire.citation.issue19pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleCellspt_PT
oaire.citation.volume13pt_PT
person.familyNameEncarnação
person.familyNameForte
person.familyNameMonteiro
person.givenNameSamuel
person.givenNamePedro
person.givenNameAntónio M.
person.identifier.ciencia-id9416-E2F5-E660
person.identifier.ciencia-id351B-B16B-79C7
person.identifier.ciencia-idC41C-6CCD-A1F0
person.identifier.orcid0000-0003-2965-2777
person.identifier.orcid0000-0003-0184-6780
person.identifier.orcid0000-0003-4467-1722
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd38d4c9f-84d5-4562-9482-5322ded17d3d
relation.isAuthorOfPublication3ecc6d1b-07a4-40d7-81f4-df6fd7b3d5b0
relation.isAuthorOfPublication5b5d8601-e683-42d5-a1b5-c8e29a4e0a41
relation.isAuthorOfPublication.latestForDiscovery5b5d8601-e683-42d5-a1b5-c8e29a4e0a41

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