Advisor(s)
Abstract(s)
To evaluate the phenolic profile and biological activity of the Algerian Sahara plant Acacia tortilis (Forssk.)
Hayne ssp. raddiana decoction and 80% ethanolic extracts were studied. Chromatographic profiling indicated
the presence of 36 phenolic compounds, including gallic acid esterified derivatives, galloylquinic derivatives and
flavan-3-ols galloyl derivatives. Both extracts showed significant cytotoxic activity and a potent anti-inflammatory
activity. They were effective against several multi-drug resistant bacteria, namely methicillin-resistant
Staphylococcus aureus (MRSA). To understand the possible mechanism of action of MRSA inhibition activity,
an in silico docking analysis was done using a virtual library of the 36 determined phenolic compounds
against penicillin-binding protein (PBP2a), a protein known to be involved in MRSA resistance to β-lactam
antibiotics. A. tortilis extracts showed interesting biological activities and the phenolic compounds found could
be an interesting starting point for the development of cytotoxic and anti-inflammatory drugs and especially
anti-MRSA antibiotics.
Description
Keywords
Acacia tortilis Anti-inflammatory Antimicrobial Cytotoxicity Molecular docking Phenolic compounds
Pedagogical Context
Citation
Ziani, Borhane E.C.; Carocho, Marcio; Abreu, Rui M.V.; Bachari, Khaldoun; Alves, Maria José; Calhelha, Ricardo C.; Talhi, Oualid; Barros, Lillian; Ferreira, Isabel C.F.R. (2020). Phenolic profiling, biological activities and in silico studies of Acacia tortilis (Forssk.) Hayne ssp. raddiana extracts. Food Bioscience. ISSN 2212-4292. 36, p. 1-10