Advisor(s)
Abstract(s)
This study aimed at investigating the chemical composition and in vitro antimicrobial activity of juniper (Juniperus communis L.) berries essential oils (EOs), including commercial samples as well as the oil hydrodistilledfromberriesgrowninPortugal,for which few information is available in the literature.The analysis was performed by gas chromatography coupled to mass spectrometry detection (GC/MS) allowing the identiïŹcation of a total of 97 compounds. The EOs showed diïŹerent chemical proïŹles with only one being according to the European Pharmacopoeia 8 requirements. The laboratory-hydrodistilled EO was characterized by its high content in α-pinene (41.6%), followed by ÎČ-pinene (27.6%) and limonene (6.4%), commercial EO1 by α-pinene (31.1%), ÎČ-myrcene (16.3%) and sabinene (7.5%) and commercial EO2 by ÎŽ-cadinene (16.0%), α-pinene (12.2%) and sabinene (9.4%). The distinct chemical proïŹles were also evidenced by principal components analysis (PCA), with a clear separation of the evaluated EOs. One of the commercial samples, showed the presence of propachlor, a banned herbicide in the European Union. All the EOs showed relevant antimicrobial activity as they presented microbicidal activity against Candida albicans and at least six of the ten tested bacteria. Commercial EO2 showed a higher biological activity, as it was active against all tested microorganisms, which could be related to its higher content in sesquiterpenes, in particular those oxygenated. Overall, results support the use of Juniper communis L. berries EO as an antiseptic in traditional medicine and highlight its potential as a biopreservative that could be used in diïŹerent industries.
Description
Keywords
Antimicrobial activity Essential oil GCâMS Juniperus communis Oxygenated sesquiterpenes
Pedagogical Context
Citation
FalcĂŁo, Soraia; BacĂ©m, Isabel; Igrejas, GetĂșlio; Rodrigues, P.J.; Vilas-Boas, Miguel; Amaral, J.S. (2018). Chemical composition and antimicrobial activity of hydrodistilled oil from juniper berries. Industrial Crops and Products. ISSN 0926-6690. 124, p. 878-884