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Biological properties of Hypericum sampsonii and Hypericum japonica: comparison of the antioxidant, anti-inflammatory and antimicrobial activities

2022Conference object Open access
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dc.contributor.authorBarciela Alvarez, Paula
dc.contributor.authorPerez Vazquez, Ana
dc.contributor.authorRodrigues, Daniele Bobrowski
dc.contributor.authorSilva, Tayse Ferreira
dc.contributor.authorMandim, Filipa
dc.contributor.authorPires, Tânia C.S.
dc.contributor.authorCarpena Rodríguez, María
dc.contributor.authorSimal-Gandara, Jesus
dc.contributor.authorBarros, Lillian
dc.contributor.authorPrieto Lage, Miguel A.
dc.date.accessioned2023-03-17T09:38:36Z
dc.date.available2023-03-17T09:38:36Z
dc.date.issued2022
dc.description.abstractHypericum was famous in ancient times for its properties, morphology, and adaptability. Currently, it has naturalized in practically all parts of the world from America to Australia. It is considered a cosmopolitan species present on all five continents. Hypericum is the largest genus of the Hypericaceae family (more than 500 species classified in 36 taxonomic sections). The study of these ethnomedicinal plants has been developed mainly and traditionally in China where there are more than 64 species, of which 19 have been related to the presence of phytochemicals with anti-tumor, anti-inflammatory, antimicrobial, antidepressant, analgesic, or neuroprotective properties, among others [1]. Remarkably, the great potential of these species has hardly been scientifically evaluated [2]. In this study, the comparison and study of the different biological properties of the species Hypericum japonica and Hypericum sampsonii was carried out. Cellular antioxidant activity was evaluated with murine macrophage cells, with both species showing 77±5% inhibition at a maximum tested concentration of 2,000 μg/mL. Antimicrobial activity was evaluated on both food contaminants and clinical bacteria and fungi. MIC and MBC were determined by colorimetric assay, where 10 μL of liquid was added from each well that showed no color change was plated on solid medium, blood agar (7% sheep blood) and incubated at 37°C for 24 h, respectively. Ampicillin and Steptomicin were used for all bacteria tested and Methicillin was also used for Staphylococcus aureus. The MIC results showed that H. japonica has a higher antimicrobial effect on Gram-negative bacteria and in Enterococcus faecalis. While H. sampsonii showed a more effective power for the rest of Gram-positive tested bacteria. The antifungal activity was performed applying the method described by Heleno et. al., 2013, using Aspergillus brasiliensis and A. fumigatus and using Ketoconazole as positive control, showing no activity for both species [3]. Anti-inflammatory activity was studied using six cell lines (CaCo2, MCF-7, NCl-460, PLP2, RAW 264.7) and Ellipticine as a control in all but RAW 264.7 where Dexamethasone was used. For all cell lines, both Hypericum species showed lower GI50 than the control, being H. japonica the one with higher anti-inflammatory effect of the two extract samples. This study is the first to provide more information on the bioactivities of two less-analyzed Hypericum speciespt_PT
dc.description.sponsorshipThe research leading to these results was supported by MICINN supporting the Ramón y Cajal grant for M.A. Prieto (RYC-2017-22891); by Xunta de Galicia for supporting the program EXCELENCIA-ED431F 2020/12 and the predoctoral grant of M. Carpena (ED481A 2021/313).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBarciela Alvarez, Paula; Perez Vazquez, Ana; Rodrigues, Daniele B.; Silva, Tayse Ferreira; Mandim, Filipa; Pires, Tânia C.S.; Carpena Rodríguez, María; Simal-Gandara, Jesus; Barros, Lillian; Prieto Lage, Miguel A. (2022). Biological properties of Hypericum sampsonii and Hypericum japonica: comparison of the antioxidant, anti-inflammatory and antimicrobial activities. In XXVI Encontro Galego-Portugues de Química: Book of Abstracts. Santiago de Compostelapt_PT
dc.identifier.isbn978-84-09-45895-0
dc.identifier.urihttp://hdl.handle.net/10198/27800
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherUniversidade de Santiago de Compostelapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHypericum sampsoniipt_PT
dc.subjectHypericum japonicapt_PT
dc.subjectResearch Subject Categories::MEDICINE::Morphology, cell biology, pathologypt_PT
dc.titleBiological properties of Hypericum sampsonii and Hypericum japonica: comparison of the antioxidant, anti-inflammatory and antimicrobial activitiespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceSantiago de Compostelapt_PT
oaire.citation.endPage357pt_PT
oaire.citation.startPage357pt_PT
oaire.citation.titleXXVI Encontro Galego-Portugues de Química: Book of Abstractspt_PT
person.familyNameRodrigues
person.familyNameMandim
person.familyNamePires
person.familyNameBarros
person.familyNamePrieto Lage
person.givenNameDaniele Bobrowski
person.givenNameFilipa
person.givenNameTânia C.S.P.
person.givenNameLillian
person.givenNameMiguel A.
person.identifier2118829
person.identifier469085
person.identifier107688
person.identifier.ciencia-idA91B-FE1D-B275
person.identifier.ciencia-idF318-9049-EB99
person.identifier.ciencia-id321E-2D96-00CA
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id221E-1E00-AB74
person.identifier.orcid0000-0003-0260-5751
person.identifier.orcid0000-0002-6494-2326
person.identifier.orcid0000-0002-3954-3833
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0002-3513-0054
person.identifier.ridQ-2842-2018
person.identifier.ridJ-3600-2013
person.identifier.ridG-4516-2011
person.identifier.scopus-author-id57057440000
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id35937495700
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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