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Advisor(s)
Abstract(s)
Cynara cardunculus L. (cardo) é uma planta herbácea nativa da bacia Mediterrânica bem adaptada à tendência das alterações climáticas devido à sua capacidade de resistir a diferentes tipos de stresse. É parte integrante da dieta mediterrânica e amplamente utilizada na medicina popular dada a sua composição nutricional e presença de compostos bioativos (responsáveis por propriedades antimicrobianas, diuréticas, coleréticas e antioxidantes), o que tem destacado o seu papel fulcral na alimentação humana. O seu potencial comercial e económico tem vindo a ser reconhecido, bem como as suas diversificadas aplicações (produção de biodiesel, pasta de papel, bioenergia, biomassa e queijo). A valorização dos seus constituintes é extremamente importante, podendo contribuir para a redução de desperdícios e para um eficiente aproveitamento dos recursos naturais. Neste estudo, sementes de cardo (var. altilis) cultivadas em Viseu (projeto CARDOP) foram separadas segundo a sua viabilidade e caracterizadas quanto à sua composição fenólica e propriedades bioativas. A composição fenólica de extratos hidrometanólicos foi analisada por HPLC-DAD-ESI/MS. Foram também avaliadas as atividades citotóxica pelo método colorimétrico da sulforrodamina B, a atividade anti-inflamatória pela inibição da produção de óxido nítrico em macrófagos de rato e a atividade antioxidante através dos ensaios TBARS e OxHLIA. Os extratos revelam a presença dos ácidos 5-O-cafeoilquínico e 3,5-O-dicafeoilquínico, ambos em maior quantidade nas sementes viáveis. O extrato destas sementes revelou também uma maior atividade antioxidante. Nenhum dos extratos apresentou citotoxicidade para células humanas tumorais e normais ou atividade anti-inflamatória. Em conclusão, as sementes viáveis destacaram-se pelos teores em ácidos fenólicos e pela atividade antioxidante.
Cynara cardunculus L. (syn. cardoon) is a herbaceous plant native to the Mediterranean basin well adapted to the trend of climate change due to its ability to withstand different types of stress. It is an integral part of the Mediterranean diet and widely used in folk medicine owing to its nutritional composition and presence of bioactive compounds (responsible for antimicrobial, diuretic, choleretic, and antioxidant properties), which has highlighted its pivotal role in human nutrition. Its commercial and economic potential has been recognized, as well as its diverse applications (biodiesel, paper pulp, bioenergy, biomass, and cheese production). The valorization of its constituents is extremely important and can contribute to the reduction of waste and the efficient use of natural resources. In this study, cardoon seeds (var. altilis) grown in Viseu (CARDOP project) were separated according to their viability and characterized for their phenolic composition and bioactive properties. The phenolic composition of hydromethanolic extracts was analysed by HPLC-DAD-ESI/MS. The cytotoxic activities were evaluated by the sulforhodamine B colorimetric method, the anti-inflammatory activity by the inhibition of nitric oxide production in murine macrophages, and the antioxidant activity by the TBARS and OxHLIA assays. The extracts reveal the presence of 5-O-caffeoylquinic and 3.5-O-dicaffeoylquinic acids, both in larger amounts in viable seeds. The extract of these seeds also revealed a higher antioxidant activity. None of the extracts showed cytotoxicity to human tumour and normal cells or anti-inflammatory activity. In conclusion, viable seeds stood out for their phenolic acid content and antioxidant activity.
Cynara cardunculus L. (syn. cardoon) is a herbaceous plant native to the Mediterranean basin well adapted to the trend of climate change due to its ability to withstand different types of stress. It is an integral part of the Mediterranean diet and widely used in folk medicine owing to its nutritional composition and presence of bioactive compounds (responsible for antimicrobial, diuretic, choleretic, and antioxidant properties), which has highlighted its pivotal role in human nutrition. Its commercial and economic potential has been recognized, as well as its diverse applications (biodiesel, paper pulp, bioenergy, biomass, and cheese production). The valorization of its constituents is extremely important and can contribute to the reduction of waste and the efficient use of natural resources. In this study, cardoon seeds (var. altilis) grown in Viseu (CARDOP project) were separated according to their viability and characterized for their phenolic composition and bioactive properties. The phenolic composition of hydromethanolic extracts was analysed by HPLC-DAD-ESI/MS. The cytotoxic activities were evaluated by the sulforhodamine B colorimetric method, the anti-inflammatory activity by the inhibition of nitric oxide production in murine macrophages, and the antioxidant activity by the TBARS and OxHLIA assays. The extracts reveal the presence of 5-O-caffeoylquinic and 3.5-O-dicaffeoylquinic acids, both in larger amounts in viable seeds. The extract of these seeds also revealed a higher antioxidant activity. None of the extracts showed cytotoxicity to human tumour and normal cells or anti-inflammatory activity. In conclusion, viable seeds stood out for their phenolic acid content and antioxidant activity.
Description
Keywords
Cynara Cardunculus L. influência da viabilidade Perfil fenólico Propriedades bioativas
Pedagogical Context
Citation
Mandim, Filipa; Dias, Maria Inês; Pinela, José; Barracosa, Paulo; Barros, Lillian; Santos-Buelga, Celestino; Ferreria, Isabel C.F.R. (2020). Perfil fenólico e propriedades bioativas de sementes viáveis e inviáveis de cardo. In V Encontro de Jovens Investigadores do Instituto Politécnico de Bragança: livro de resumos. Bragança: Instituto Politécnico. ISBN 978-972-745-282-8
Publisher
Instituto Politécnico de Bragança