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Optimization of ergosterol extraction from Pleurotus mushrooms using response surface methodology (RSM)

2019Conference object Open access
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dc.contributor.authorTaofiq, Oludemi
dc.contributor.authorSilva, Ana Rita
dc.contributor.authorCosta, Cristina
dc.contributor.authorPrieto Lage, Miguel A.
dc.contributor.authorBarros, Joana
dc.contributor.authorFerreira, Inês
dc.contributor.authorNunes, João
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2021-10-22T13:12:44Z
dc.date.available2021-10-22T13:12:44Z
dc.date.issued2019
dc.description.abstractPleurotus genus represents one of the most widely consumed mushrooms in the world with interesting healthpromoting benefits, mainly due to their richness in several bioactive compounds [1]. Mushrooms produce ergosterol as one of their main sterols, which has been considered a contributor to their anti-inflammatory, antioxidant, and antitumor properties [2]. Obtaining an ergosterol enriched extract depends on different variables, such as the extraction method, solvent type, temperature, extraction time, and the solid-liquid ratio [2]. Therefore, it is essential to define the main variables and relevant response criteria to maximize the extraction yield and purity, taking into account cost functions. In the present work, response surface methodology (RSM) was applied to optimize a heat assisted extraction system (HAE), combining time (t) and temperature (T) effects, and using a circumscribed central composite design (CCCD) for the recovery of ergosterol from the fruiting bodies of P. ostreatus and P. eryngii produced with lignocellulose substrate. Both mushrooms (POG and PEG for P. ostreatus and P. eryngii) and their byproduct (POR and PER for P. ostreatus and P. eryngii) were used in the present work. The obtained responses were the quantification of ergosterol by HPLC-UV (Y1: mg of ergosterol per g of extract residue and Y2: mg of ergosterol per 100 g of dry weight mushroom).pt_PT
dc.description.sponsorshipThe authors are grateful to FCT, Portugal and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019); European Regional Development Fund (ERDF) within the scope of project MicoBioExtract (POCI-01- 0247-FEDER- 033939); European Agricultural Fund for Rural Development (EAFRD), through the Rural Development Program (PDR2020), within the scope of Project MicoCoating (PDR2020-101-031472); European Structural and Investment Funds (FEEI) through the Regional Operational Program North 2020, within the scope of Project Mobilizador ValorNatural®; L. Barros. thanks the national funding by FCT, P.I., through the institutional scientific employment program-contract for her contract.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTaofiq, Oludemi; Silva, Ana Rita; Costa, Cristina; Prieto Lage, Miguel A.; Barros, Joana; Ferreira, Inês; Nunes, João; Barros, Lillian; Ferreira, Isabel C.F.R. (2019). Optimization of ergosterol extraction from Pleurotus mushrooms using response surface methodology (RSM). In XXV Encontro Galego-Portugués de Química. ISBN 978-84-09-16320-5pt_PT
dc.identifier.isbn978-84-09-16320-5
dc.identifier.urihttp://hdl.handle.net/10198/24069
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPleurotus mushroomspt_PT
dc.subjectErgosterolpt_PT
dc.titleOptimization of ergosterol extraction from Pleurotus mushrooms using response surface methodology (RSM)pt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2019/PT
oaire.citation.conferencePlaceSantiago de Compostela, Espanhãpt_PT
oaire.citation.titleXXV Encontro Galego-Português de Químicapt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSilva
person.familyNamePrieto Lage
person.familyNameBarros
person.familyNameFerreira
person.givenNameAna Rita
person.givenNameMiguel A.
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifierABD-5857-2020
person.identifier107688
person.identifier469085
person.identifier144781
person.identifier.ciencia-id0E1A-35C1-C79A
person.identifier.ciencia-id221E-1E00-AB74
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-5819-9065
person.identifier.orcid0000-0002-3513-0054
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridG-4516-2011
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id35937495700
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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