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Advisor(s)
Abstract(s)
The objectives of this work were to assess the genetic diversity within and between three Portuguese cattle breeds using
93 microsatellites markers. Blood samples were collected from 50 individuals of each breed, and ninety-three
microsatellites were analysed to get thorough information about genetic diversity and interrelationships among three
Portuguese cattle breeds: Mirandesa (MIR), Maronesa (MAR), and Barrosã (BAR). Estimates of genetic variability,
observed (Ho) and expected heterozygosity (He), allelic richness for each locus were determined. The alleles were
classified in three classes according to their frequency: common alleles (observed in the three sub-populations), private
alleles (alleles observed in one sub-population) and rare alleles (non-private alleles with a frequency < 0.01 over the
whole population). The number of rare alleles found was 52 in MAR, 33 in MIR, and 30 in BAR. The number of private
alleles found was 5 in MIR and BAR, and 2 in MAR. The MIR showed the lowest genetic diversity, and the highest
genetic distance to the other two breeds. The three breeds could be considered as genetically distinct populations. This
study shows that measures should be taken in order to preserve the genetic diversity of MIR, MAR, and BAR cattle
breeds.
Description
Keywords
Cattle Conservation Genetic diversity Microsatellites
Pedagogical Context
Citation
Almeida, Armandina; Araújo, José P.P.; Medurogac, Ivica; Cadavez, Vasco (2012). Study of genetic diversity of three portuguese cattle breeds by 93 micro satellite markers. Scientific Papers. Series D. Animal Science. ISSN 2285-5750. 56, p. 41-47