Advisor(s)
Abstract(s)
Eryngium viviparum is an endangered species that requires management efforts based on complementary ex situ
conservation strategies, such as in vitro culture. This study was carried out to evaluate the phenolic composition
and the antioxidant, cytotoxic, and antimicrobial properties of E. viviparum aerial and root parts obtained by this
micropropagation technique. The HPLC-DAD-ESI/MSn analysis showed that phenolic compounds were more
abundant in the root (102 ± 4 mg g−1 extract) than in the aerial part (40.6 ± 0.8 mg g−1 extract) of the plant.
The major compound was trans rosmarinic acid, followed by trans 3-O-caffeoylquinic acid. The root extract also
showed higher antioxidant activity, with a result close to that of trolox in the thiobarbituric acid reactive
substances (TBARS) formation inhibition assay, and a moderate toxicity to lung (NCI-H460), breast (MCF-7) and
liver (HepG2) tumour cells. It was also more effective than ketoconazole against Penicillium ochrochloron. In turn,
the aerial part extract inhibited Salmonella typhimurium more effectively than ampicillin. This study highlights E.
viviparum as an unexplored source of bioactive compounds with potential application in the food, pharmaceutical,
and other industrial sectors. Consequently, it promotes the interest of conserving this endangered species.
Description
Keywords
Apiaceae Bioactive properties Ex situ conservation Herbal plants In vitro culture Phenolic acids
Pedagogical Context
Citation
Ayuso, Manuel; Pinela, José; Dias, Maria Inês; Barros, Lillian; Ivanov, Marija; Calhelha, Ricardo C.; Soković, Marina; Ramil-Rego, Pablo; Barreal, M. Esther; Gallego, Pedro Pablo; Ferreira, Isabel C.F.R. (2020). Phenolic composition and biological activities of the in vitro cultured endangered Eryngium viviparum J. Gay. Industrial Crops and Products. ISSN 0926-6690. 148, p. 1-7