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Label-free multi-step microfluidic device for mechanical characterization of blood cells: diabetes type II

2022Journal article Open access
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dc.contributor.authorPinho, Diana
dc.contributor.authorFaustino, Vera
dc.contributor.authorCatarino, Susana
dc.contributor.authorPereira, Ana I.
dc.contributor.authorMinas, Graça
dc.contributor.authorPinho, Fernando T.
dc.contributor.authorLima, Rui A.
dc.date.accessioned2023-02-24T11:46:19Z
dc.date.available2023-02-24T11:46:19Z
dc.date.issued2022
dc.description.abstractThe increasing interest to establish significant correlations between blood cell mechanical measurements and blood diseases, has led to the promotion of microfluidic devices as attractive clinical tools for potential use in diagnosis. A multi-step microfluidic device able to separate red and white blood cells (RBCs and WBCs) from plasma and simultaneously measure blood cells deformability (5 and 20% of hematocrit) is presented in this paper. The device employs passive separation based on the cross-flow filtration principle, introduced at each daughter channel. At the outlets, hyperbolic geometries allow single-cell deformability analysis. The device was tested with blood from five healthy and fifteen diabetic type II voluntary donors. The results have shown that WBCs have lower deformability than RBCs, and no significant differences were observed in WBCs from healthy and pathological blood samples. In contrast, RBCs have shown significant differences, with pathological cells exhibiting lower deformability. Shear rheology has shown that blood from patients with type II diabetes has higher viscosity than blood from healthy donors. This microfluidic device has demonstrated the ability to reduce cell concentration at the outlets down to 1%, an ideal cell concentration for assessing the blood cells deformability, under healthy and pathological conditions. The results provide new insights and quantitative information about the hemodynamics of in vitro type II diabetes mellitus RBCs. Thus, such device can be a promising complement in clinical diagnosis and biological research as part of an integrated blood-on-a-chip system.pt_PT
dc.description.sponsorshipThis work was also supported by Fundação para a Ciência e a Tecnologia (FCT) under the strategic grants UIDB/04077/2020 and UIDB/00532/2020. D. Pinho and V. Faustino acknowledge the Ph.D. scholarships SFRH/BD/89077/2012 and SFRH/BD/99696/2014, respectively, both provided by FCT. Susana Catarino thanks FCT for her contract funding provided through 2020.00215.CEECIND. F. T. Pinho is thankful to FCT for financial support through projects LA/P/0045/2020 of the Associate Laboratory in Chemical Engineering (ALiCE) and projects UIDB/00532/2020 and UIDP/00532/2020 of Centro de Estudos de Fenomenos de Transporte.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPinho, Diana; Faustino, Vera; Catarino, S.O.; Pereira, Ana I.; Minas, G.; Pinho, Fernando; Lima, R. (2022). Label-free multi-step microfluidic device for mechanical characterization of blood cells: diabetes type II. Micro and Nano Engineeringpt_PT
dc.identifier.doi10.1016/j.mne.2022.100149pt_PT
dc.identifier.urihttp://hdl.handle.net/10198/27173
dc.language.isoengpt_PT
dc.relationMechanical Engineering and Resource Sustainability Center
dc.relationTransport Phenomena Research Center
dc.relationBLOOD RHEOLOGY AND RED BLOOD CELL MIGRATION IN MICROCHANNEL FLOWS
dc.relationMicrofluidic system for cell separation and deformation assessment based on biomimetic and acoustofluidic methods
dc.relationTransport Phenomena Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHydrodynamic separationpt_PT
dc.subjectBlood-on-a-chippt_PT
dc.subjectViscositypt_PT
dc.subjectDeformabilitypt_PT
dc.subjectDiabetes type IIpt_PT
dc.subjectBiomicrofluidicspt_PT
dc.titleLabel-free multi-step microfluidic device for mechanical characterization of blood cells: diabetes type IIpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMechanical Engineering and Resource Sustainability Center
oaire.awardTitleTransport Phenomena Research Center
oaire.awardTitleBLOOD RHEOLOGY AND RED BLOOD CELL MIGRATION IN MICROCHANNEL FLOWS
oaire.awardTitleMicrofluidic system for cell separation and deformation assessment based on biomimetic and acoustofluidic methods
oaire.awardTitleTransport Phenomena Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04077%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00532%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F89077%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F99696%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00532%2F2020/PT
oaire.citation.titleMicro and Nano Engineeringpt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamFARH
oaire.fundingStreamFARH
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePinho
person.familyNamePereira
person.givenNameDiana
person.givenNameAna I.
person.identifier.ciencia-id5214-ED0C-35E8
person.identifier.ciencia-id0716-B7C2-93E4
person.identifier.orcid0000-0002-3884-6496
person.identifier.orcid0000-0003-3803-2043
person.identifier.ridF-3168-2010
person.identifier.scopus-author-id15071961600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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