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Abstract(s)
Este estudo, teve como objetivo analisar, as propriedades bioativas e a composição
química de diferentes partes da planta Humulus lupulus L. (lúpulo), incluindo flores,
folhas e rizomas de duas cultivares (Cascade e Nugget) e um Espontâneo. Os principais
parâmetros analisados, foram a capacidade antioxidante, a composição de compostos
fenólicos, além da atividade antibacteriana e antifúngica dos extratos.
Os resultados demonstraram, que o rizoma da cultivar Nugget, apresentou a maior
atividade antioxidante, com um IC50 de 0,0635 mg/mL no ensaio DPPH, seguido pelo
rizoma do Espontâneo com 0,079 mg/mL. As folhas também exibiram uma atividade
antioxidante, com o IC50 variando entre 0,25 mg/mL (Cascade) e 0,077 mg/mL
(Espontâneo). Por outro lado, as flores apresentaram a menor capacidade antioxidante,
com valores de IC50 de 0,164 mg/mL (Espontâneo) e 0,39 mg/mL (Nugget). No ensaio
de TBARS, que determina a inibição da peroxidação lipídica, os rizomas também se
destacaram com 0,0004 mg/mL (Nugget).
Em termos de compostos fenólicos, as flores destacaram-se, por apresentar a maior
quantidade de compostos fenólicos totais a variar entre 5,74 mg/g (Cascade) e 7,2mg/g
(Nugget). A flor da cultivar Cascade, apresentou o maior teor de ácidos fenólicos totais
(2,04 mg/g), enquanto o maior conteúdo de flavonoides, foi encontrado nas folhas do
Espontâneo (5,5 mg/g). O rizoma também se destacou pela presença de (+) -Catequina,
com concentrações de 0,054 mg/g (Cascade), 0,089 mg/g (Espontâneo) e 0,051mg/g
(Nugget), compostos que são reconhecidos pelo seu forte potencial antioxidante.
A avaliação da atividade antimicrobiana, mostrou variabilidade entre as diferentes partes
da planta. Para as duas cultivares e para o espontâneo avaliadas, o rizoma foi a parte da
planta que se destacou com maior atividade antimicrobiana, especialmente contra
bactérias Gram-positivas, como Listeria monocytogenes com valores de CMI de 0,3
mg/mL na cultivar Cascade e Espontâneo e Enterococcus faecalis com valores de CMI
de 2,5 mg/mL (Nugget) e 0,3 mg/mL (Cascade). Também mostrou eficácia moderada,
contra bactérias Gram-negativas, como Morganella morganii (CMI de 0,6 mg/mL). Há
uma variabilidade considerável, na atividade antifúngica dos extratos das diferentes
partes das plantas de lúpulo, com destaque para os extratos de folha e rizoma, que
demonstraram melhor potencial antifúngico. É de salientar que apenas o Espontâneo
apresentou ação fungistática para o Aspergillus brasiliensis com valores de CMIs entre
2,5 mg/mL (rizoma) e 5mg/mL (folha). Esses resultados indicam que o rizoma de Humulus lupulus L. possui um potencial antioxidante e antimicrobiano promissor, o que
pode ser explorado em diversas indústrias, como a farmacêutica e alimentar. As folhas,
por sua vez, demonstram um alto teor de compostos bioativos, especialmente os
fenólicos, que também têm potencial para aplicações futuras.
This study aimed to analyze the bioactive properties and chemical composition of different parts of the plant Humulus lupulus L. (lupulus), including flowers, leaves and rhizomes of two cultivars (Cascade and Nugget), and Spontaneous. The main parameters analyzed were the antioxidant capacity, the composition of phenolic compounds, in addition to the antibacterial and antifungal activity of the extracts. The results demonstrated that the rhizome of the Nugget cultivars presented the highest antioxidant activity, with an IC50 of 0.0635 mg/mL in the DPPH assay, followed by the rhizome of the Spontaneo 0.079 mg/mL. The leaves also exhibited antioxidant activity with the IC50 ranging between 0.25 mg/mL (Cascade) and 0.077 mg/mL (Spontaneous). On the other hand, flowers had the lowest antioxidant capacity, with IC50 values of 0.164 mg/mL (Spontaneous) and 0.39 mg/mL (Nugget). In the TBARS assay, which determines the inhibition of lipid peroxidation, the rhizomes also stood out at 0.0004 mg/mL (Nugget). In terms of phenolic compounds, the flowers stood out, as they presented the highest amount of total phenolic compounds, ranging between 5.74 mg/g (Cascade) and 7.2 mg/g (Nugget). The flower of the Cascade cultivar had the highest content of total phenolic acids (2.04 mg/g) while the highest content of flavonoids was found in the leaves of the Spontaneo (5.5 mg/g). The rhizome also stood out for the presence of (+)-Catechin, with concentrations of 0.054 mg/g (Cascade), 0.089 mg/g (Spontaneous) and 0.051 mg/g (Nugget), compounds that are recognized for their strong antioxidant potential. The evaluation of antimicrobial activity showed variability between different parts of the plant. For all cultivars and spontaneously evaluated, the rhizome was the part of the plant that stood out with the greatest antimicrobial activity, especially against Gram-positive bacteria, such as Listeria monocytogenes with MIC values of 0.3 mg/mL in cultivars Cascade and Spontaneo and Enterococcus faecalis with MIC values of 2.5 mg/mL (Nugget) and 0.3 mg/mL (Cascade). It also showed moderate efficacy against Gram- negative bacteria such as Morganella morganii (MIC of 0.6 mg/mL). There is considerable variability in the antifungal activity of extracts of the different parts of lupulus plants, with emphasis on leaf and rhizome extracts, which demonstrated better antifungal potential. It should be noted that only the spontaneous variety showed fungistatic action against Aspergillus brasiliensis with MIC values between 2.5 mg/mL (rhizome) and 5mg/mL (leaf). These results indicate that the rhizome of Humulus lupulus L. has a promising antioxidant and antimicrobial potential, which can be explored in various industries, such as pharmaceutical and food. The leaves, in turn, demonstrate a high content of bioactive compounds, especially phenolics, which also have potential for future applications.
This study aimed to analyze the bioactive properties and chemical composition of different parts of the plant Humulus lupulus L. (lupulus), including flowers, leaves and rhizomes of two cultivars (Cascade and Nugget), and Spontaneous. The main parameters analyzed were the antioxidant capacity, the composition of phenolic compounds, in addition to the antibacterial and antifungal activity of the extracts. The results demonstrated that the rhizome of the Nugget cultivars presented the highest antioxidant activity, with an IC50 of 0.0635 mg/mL in the DPPH assay, followed by the rhizome of the Spontaneo 0.079 mg/mL. The leaves also exhibited antioxidant activity with the IC50 ranging between 0.25 mg/mL (Cascade) and 0.077 mg/mL (Spontaneous). On the other hand, flowers had the lowest antioxidant capacity, with IC50 values of 0.164 mg/mL (Spontaneous) and 0.39 mg/mL (Nugget). In the TBARS assay, which determines the inhibition of lipid peroxidation, the rhizomes also stood out at 0.0004 mg/mL (Nugget). In terms of phenolic compounds, the flowers stood out, as they presented the highest amount of total phenolic compounds, ranging between 5.74 mg/g (Cascade) and 7.2 mg/g (Nugget). The flower of the Cascade cultivar had the highest content of total phenolic acids (2.04 mg/g) while the highest content of flavonoids was found in the leaves of the Spontaneo (5.5 mg/g). The rhizome also stood out for the presence of (+)-Catechin, with concentrations of 0.054 mg/g (Cascade), 0.089 mg/g (Spontaneous) and 0.051 mg/g (Nugget), compounds that are recognized for their strong antioxidant potential. The evaluation of antimicrobial activity showed variability between different parts of the plant. For all cultivars and spontaneously evaluated, the rhizome was the part of the plant that stood out with the greatest antimicrobial activity, especially against Gram-positive bacteria, such as Listeria monocytogenes with MIC values of 0.3 mg/mL in cultivars Cascade and Spontaneo and Enterococcus faecalis with MIC values of 2.5 mg/mL (Nugget) and 0.3 mg/mL (Cascade). It also showed moderate efficacy against Gram- negative bacteria such as Morganella morganii (MIC of 0.6 mg/mL). There is considerable variability in the antifungal activity of extracts of the different parts of lupulus plants, with emphasis on leaf and rhizome extracts, which demonstrated better antifungal potential. It should be noted that only the spontaneous variety showed fungistatic action against Aspergillus brasiliensis with MIC values between 2.5 mg/mL (rhizome) and 5mg/mL (leaf). These results indicate that the rhizome of Humulus lupulus L. has a promising antioxidant and antimicrobial potential, which can be explored in various industries, such as pharmaceutical and food. The leaves, in turn, demonstrate a high content of bioactive compounds, especially phenolics, which also have potential for future applications.
Description
Keywords
Humulus lupulus L. Rizoma Antioxidante Antimicrobiana Compostos fenólicos