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Este trabalho foi realizado na cidade de Bragança (Portugal), com o intuito de estudar
os efeitos da dose de prostaglandina F2 (PGF2α) associada a um tratamento de progestagênico
curto + gonadotrofina coriônica equina (eCG) de sincronização de estros e do tempo de
conservação do sêmen a 5ºC. Além disso, foram avaliados alguns parâmetros quanto a
características seminais, local de deposição do sêmen e refluxo seminal.
O estudo foi feito com 66 ovelhas da raça Churra Galega Bragançana. O tratamento
progestagênico curto foi realizado com a utilização de um dispositivo vaginal com 0,35 g de
progesterona (CIDR), e concomitante à inserção do dispositivo, metade delas recebeu uma
aplicação intramuscular de 75 μg de cloprostenol, e a outra metade recebeu 100 μg de
cloprostenol. O tratamento progestagênico teve duração de sete dias, e no momento da retirada
do dispositivo, todas as ovelhas receberam uma dose intramuscular de 500 UI de eCG. Todos
os animais responderam ao tratamento progestagênico. O sêmen foi recolhido via
eletroejaculação e foi refrigerado por 2 dias, 1 dia e 0 dias. Após 41 dias, todas as ovelhas foram
submetidas a detecção de prenhez por ultrassonografia. A essa altura, 78,8% dos animais
estavam gestantes.
No final do ensaio, concluiu-se que o uso de 100 μg de PGF2α foi mais eficaz que o uso
de 75 μg, com taxas de fertilidade de 84,8% e 72,7%, respectivamente. O tempo de refrigeração
(0 dias, 1 dia e 2 dias) não interferiu no resultado de fertilidade (P> 0,05), com taxas de 81,0%,
82,6% e 72,7%, respectivamente. Por sua vez, o local de deposição do sêmen apresentou
melhores resultados quando depositado no corpo do útero (100,0%), do que na cérvix (77,8%).
O refluxo seminal não apresentou variação significativa (P>0,05) na fertilidade, em que 6,3%
dos animais não apresentaram refluxo de sêmen e obtiveram taxa de fertilidade de 75,0%;
85,7% das ovelhas tiveram refluxo ligeiro e taxa de fertilidade de 77,8%; e 7,9% apresentaram
refluxo moderado com taxa de fertilidade de 80,0%.
This study was conducted in the city of Bragança (Portugal) with the porpose of evaluating the effectiveness of a reduced dose of prostaglandin (PGF-2α) combined with a short progestogen treatment and equine chorionic gonadotrophin (eCG) in synchronizing estrus and semen storage time at 5ºC. Additionally, various parameters were assessed regarding seminal characteristics, semen deposition site and seminal reflux. The research was carried out using 66 Churra Galega Bragançana breed sheep. The short progestogen treatment involved the use of a vaginal device containing 0.35 g of progesterone (CIDR). Concurrent with the device insertion, half of the sheep received an intramuscular injection of 75 μg of Cloprostenol, while the other half received 100 μg of Cloprostenol. The progestogen treatment lasted for 7 days. Upon the removal of the device, all ewes received a 500 IU intramuscular injection of eCG. All animals responded positively to the progestogen treatment. Semen was collected through electroejaculation and was refrigerated for 2 days, 1 day and 0 days. After 41 days, ultrasound was used to diagnose pregnancy in all ewes, resulting in a pregnancy rate of 78.8%. After the experiment, it was determined that the use of 100 μg of PGF2αwas more effective than 75 μg, with fertility rates of 84.8% and 72.7%, respectively. The refrigeration time (0 days, 1 day and 2 days) did not significantly impact the fertility results (P>0.05), with rates of 81.0%, 82.6%, and 72.7% respectively. In contrast, uterus semen deposition yielded better results (100.0%) than cervix semen deposition (77.8%). Seminal reflux did not exhibit a significant (P>0.05) variation in fertility, with 6.3% of animals experiencing no semen reflux and a fertility rate of 75%; 85.7% of the ewes showing slight reflux and a fertility rate of 77.8%; and 7.9% with moderate reflux and a fertility rate of 80.0%.
This study was conducted in the city of Bragança (Portugal) with the porpose of evaluating the effectiveness of a reduced dose of prostaglandin (PGF-2α) combined with a short progestogen treatment and equine chorionic gonadotrophin (eCG) in synchronizing estrus and semen storage time at 5ºC. Additionally, various parameters were assessed regarding seminal characteristics, semen deposition site and seminal reflux. The research was carried out using 66 Churra Galega Bragançana breed sheep. The short progestogen treatment involved the use of a vaginal device containing 0.35 g of progesterone (CIDR). Concurrent with the device insertion, half of the sheep received an intramuscular injection of 75 μg of Cloprostenol, while the other half received 100 μg of Cloprostenol. The progestogen treatment lasted for 7 days. Upon the removal of the device, all ewes received a 500 IU intramuscular injection of eCG. All animals responded positively to the progestogen treatment. Semen was collected through electroejaculation and was refrigerated for 2 days, 1 day and 0 days. After 41 days, ultrasound was used to diagnose pregnancy in all ewes, resulting in a pregnancy rate of 78.8%. After the experiment, it was determined that the use of 100 μg of PGF2αwas more effective than 75 μg, with fertility rates of 84.8% and 72.7%, respectively. The refrigeration time (0 days, 1 day and 2 days) did not significantly impact the fertility results (P>0.05), with rates of 81.0%, 82.6%, and 72.7% respectively. In contrast, uterus semen deposition yielded better results (100.0%) than cervix semen deposition (77.8%). Seminal reflux did not exhibit a significant (P>0.05) variation in fertility, with 6.3% of animals experiencing no semen reflux and a fertility rate of 75%; 85.7% of the ewes showing slight reflux and a fertility rate of 77.8%; and 7.9% with moderate reflux and a fertility rate of 80.0%.
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Ovinos Prostaglandina Sincronização de estro Conservação do sêmen