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Authors
Abstract(s)
A geoprópolis tem sido usada na medicina tradicional há séculos. Neste estudo foram avaliadas a
origem botânica, as características físico-químicas, o perfil fenólico e as atividades biológicas da
geoprópolis de Melipona scutellaris colhida nos períodos chuvoso e seco. A análise palinológica
permitiu identificar mais de 50 tipos polínicos, sendo Schinus terebinthifolius e Cecropia os tipos
predominantes. A geoprópolis da estação chuvosa apresentou maior teor total de fenóis e
flavonoides (determinado por métodos convencionais – 25,13% e 3,92%, respetivamente) em
comparação à estação seca (19,30% e 2,09%); os principais picos (determinados por
Cromatografia líquida de alta Eficiência (naringina, ácido gálico e catequina) foram semelhantes
nas amostras. A capacidade antioxidante foi avaliada através de ensaios de DPPH, poder redutor
e descoloração de β-caroteno/ácido linoleico. As amostras da estação chuvosa apresentaram
atividade antioxidante superior independentemente do método utilizado. O efeito antimicrobiano
foi avaliado por microdiluição, enquanto o impacto na enzima colinesterase foi quantificado pela
acumulação do ácido 5-tio 2-nitrobenzóico. As atividades anti-inflamatórias e antimutagénicas
foram quantificadas através da inibição da enzima hialuronidase e utilizando células
Saccharomyces cerevisiae ATCC-20113. Ambas as amostras exibiram propriedades anti-
inflamatórias e antimutagénicas. Foi também, observada uma inibição significativa da
acetilcolinesterase, com valores de IC50 de 0,35 µg/mL durante a estação chuvosa e 0,28 µg/mL
durante a estação seca. Adicionalmente, a geoprópolis apresentou atividade antimicrobiana,
principalmente contra Staphylococcus aureus, ATCC-4330. Os nossos resultados sugerem o
potencial terapêutico da geoprópolis de M. scutellaris no contexto de doenças inflamatórias,
oxidativas e infeciosas.
Geopropolis has been used in traditional medicine for centuries. This study evaluated the botanical origin, physicochemical characteristics, phenolic profile and biological activities of Melipona scutellaris geopropolis collected during the rainy and dry seasons. The palynological analysis identified more than 50 pollen species, with Schinus terebinthifolius and Cecropia being the most abundant. The geopropolis from the wet season had a higher total phenolic and flavonoid content (determined by conventional methods - 25.13% and 3.92%, respectively) compared to the dry season (19.30% and 2.09%); the main peaks (determined by high performance liquid chromatography (HPLC) (naringin, gallic acid and catechin) were similar in the samples. Antioxidant capacity was assessed using DPPH, reducing power and β-carotene/lyleic acid decolorization tests. Rainy season samples showed higher antioxidant activity regardless of the method used. The antimicrobial effect was assessed by microdilution, while the effect on the enzyme cholinesterase was quantified by the accumulation of 5-thio-2-nitrobenzoic acid. The anti- inflammatory and antimutagenic activities were quantified by inhibiting the enzyme hyaluronidase and using Saccharomyces cerevisiae ATCC-20113 cells. Both samples showed anti-inflammatory and antimutagenic properties. Significant inhibition of acetylcholinesterase was also observed, with IC50 values of 0.35 µg/mL during wet and 0.28 µg/mL during dry seasons. In addition, geopropolis showed antimicrobial activity, mainly against Staphylococcus aureus ATCC-4330. Our results suggest the therapeutic potential of M. scutellaris geopropolis in the context of inflammatory, oxidative and infectious diseases.
Geopropolis has been used in traditional medicine for centuries. This study evaluated the botanical origin, physicochemical characteristics, phenolic profile and biological activities of Melipona scutellaris geopropolis collected during the rainy and dry seasons. The palynological analysis identified more than 50 pollen species, with Schinus terebinthifolius and Cecropia being the most abundant. The geopropolis from the wet season had a higher total phenolic and flavonoid content (determined by conventional methods - 25.13% and 3.92%, respectively) compared to the dry season (19.30% and 2.09%); the main peaks (determined by high performance liquid chromatography (HPLC) (naringin, gallic acid and catechin) were similar in the samples. Antioxidant capacity was assessed using DPPH, reducing power and β-carotene/lyleic acid decolorization tests. Rainy season samples showed higher antioxidant activity regardless of the method used. The antimicrobial effect was assessed by microdilution, while the effect on the enzyme cholinesterase was quantified by the accumulation of 5-thio-2-nitrobenzoic acid. The anti- inflammatory and antimutagenic activities were quantified by inhibiting the enzyme hyaluronidase and using Saccharomyces cerevisiae ATCC-20113 cells. Both samples showed anti-inflammatory and antimutagenic properties. Significant inhibition of acetylcholinesterase was also observed, with IC50 values of 0.35 µg/mL during wet and 0.28 µg/mL during dry seasons. In addition, geopropolis showed antimicrobial activity, mainly against Staphylococcus aureus ATCC-4330. Our results suggest the therapeutic potential of M. scutellaris geopropolis in the context of inflammatory, oxidative and infectious diseases.
Description
Keywords
Produtos apícolas Geoprópolis Produtos naturais Melipona Scutellaris