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Botanical authentication of globe artichoke-containing foods: Differentiation of Cynara scolymus by a novel HRM approach

2022Journal article Open access
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dc.contributor.authorGrazina, Liliana
dc.contributor.authorBatista, Andreia
dc.contributor.authorAmaral, Joana S.
dc.contributor.authorCosta, Joana
dc.contributor.authorMafra, Isabel
dc.date.accessioned2021-08-31T10:27:23Z
dc.date.available2021-08-31T10:27:23Z
dc.date.issued2022
dc.description.abstractCynara scolymus L., known as globe artichoke, is a medicinal plant widely used in plant food supplements (PFS) and herbal infusions due to its beneficial health properties. The high demand for artichoke-containing products can lead to adulteration practices. In this work, a real-time polymerase chain reaction (PCR) system coupled to high-resolution melting (HRM) analysis was proposed to differentiate C. scolymus from other Cynara species. Hence, a Cynara-specific real-time PCR assay was successfully developed with high analytical performance, achieving a sensitivity of 0.4 pg of globe artichoke DNA. HRM analysis enabled the discrimination of C. scolymus, with a high level of confidence (>98%), corroborating sequencing data. Application results to artichokecontaining PFS and mixed herbal infusions allowed confirming the presence of C. scolymus in 38% of the samples, suggesting the substitution/mislabelling of globe artichoke in 2 samples and the need for further efforts to increase DNA amplifiability of PFS.pt_PT
dc.description.sponsorshipThe authors are grateful to the University of Lisbon and Jardim Botˆanico de Coimbra (Portugal), Real Jardin Bot´anico Juan Carlos (Spain), Seed Conservation Department of Royal Botanic Garden (UK) and Jardin des plantes et Botanique (France) for the supply of plant material. The authors acknowledge the support of the project UIDB/00690/2020, subsidised by FCT/MCTES (Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through national funds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGrazina, Liliana; Batista, Andreia; Amaral, Joana S.; Costa, Joana; Mafra, Isabel (2022). Botanical authentication of globe artichoke-containing foods: Differentiation of Cynara scolymus by a novel HRM approach. Food Chemistry. ISSN 0308-8146. 366, p. 1-9pt_PT
dc.identifier.doi10.1016/j.foodchem.2021.130621pt_PT
dc.identifier.issn0308-8146
dc.identifier.urihttp://hdl.handle.net/10198/23840
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGlobe artichokept_PT
dc.subjectPlant food supplementspt_PT
dc.subjectAuthenticitypt_PT
dc.subjectReal-time PCRpt_PT
dc.subjectHigh-resolution melting analysispt_PT
dc.titleBotanical authentication of globe artichoke-containing foods: Differentiation of Cynara scolymus by a novel HRM approachpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.citation.startPage130621pt_PT
oaire.citation.titleFood Chemistrypt_PT
oaire.citation.volume366pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAmaral
person.givenNameJoana S.
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-3648-7303
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isAuthorOfPublication.latestForDiscovery42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isProjectOfPublication29718e93-4989-42bb-bcbc-4daff3870b25
relation.isProjectOfPublication.latestForDiscovery29718e93-4989-42bb-bcbc-4daff3870b25

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