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Advisor(s)
Abstract(s)
Cynara scolymus L., known as globe artichoke, is a medicinal plant widely used in plant food supplements (PFS)
and herbal infusions due to its beneficial health properties. The high demand for artichoke-containing products
can lead to adulteration practices. In this work, a real-time polymerase chain reaction (PCR) system coupled to
high-resolution melting (HRM) analysis was proposed to differentiate C. scolymus from other Cynara species.
Hence, a Cynara-specific real-time PCR assay was successfully developed with high analytical performance,
achieving a sensitivity of 0.4 pg of globe artichoke DNA. HRM analysis enabled the discrimination of C. scolymus,
with a high level of confidence (>98%), corroborating sequencing data. Application results to artichokecontaining
PFS and mixed herbal infusions allowed confirming the presence of C. scolymus in 38% of the samples,
suggesting the substitution/mislabelling of globe artichoke in 2 samples and the need for further efforts to
increase DNA amplifiability of PFS.
Description
Keywords
Globe artichoke Plant food supplements Authenticity Real-time PCR High-resolution melting analysis
Citation
Grazina, Liliana; Batista, Andreia; Amaral, Joana S.; Costa, Joana; Mafra, Isabel (2022). Botanical authentication of globe artichoke-containing foods: Differentiation of Cynara scolymus by a novel HRM approach. Food Chemistry. ISSN 0308-8146. 366, p. 1-9