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Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies

2019Journal article Restricted access
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dc.contributor.authorRamalhosa, Elsa
dc.contributor.authorFernandes, Luana
dc.contributor.authorPereira, J.A.
dc.contributor.authorSaraiva, Jorge A.
dc.contributor.authorCasal, Susana
dc.date.accessioned2020-03-13T12:14:46Z
dc.date.available2020-03-13T12:14:46Z
dc.date.issued2019
dc.description.abstractIntroduction: Pansies (Viola×wittrockiana) are a rich source of natural antioxidants with beneficial effects on human health. Objetives: The aim of our study was to investigate solvents’ influence (water, methanol, water:acetone (6:4, v/v)) on the extraction of bioactive compounds and antioxidant activity of pansies extracts. Methods: The bioactive compounds analyzed were the following: flavonoids, hydrolysable tannins and monomeric anthocyanins, as well as total phenols by the total reducing capacity assay (TRC). The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power assays. A Principal Component Analysis (PCA) was performed to differentiate pansies extracts. Results: The solvents that yielded extracts with the highest contents of hydrolysable tannins and TRC were methanol and water:acetone (6:4, v/v). To extract the highest contents of monomeric anthocyanins, methanol should be used (5.93 mg Cy 3-glu/g flower, d.w), while for flavonoids, water:acetone (6:4, v/v) was the preferred yielding an extract with 115 mg QE/g flower d.w. Water turned out to be the least effective solvent, giving extracts with the lowest antioxidant activity. In addition, methanol or water:acetone extracts were clearly distinguished from aqueous ones through a PCA analysis. Conclusions: Our results show that the bioactive compounds and antioxidant activity of pansies’ extracts are affected by the solvent used.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.29352/mill0208.08.00205pt_PT
dc.identifier.urihttp://hdl.handle.net/10198/20975
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPansiespt_PT
dc.subjectSolventspt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectBioactive compoundspt_PT
dc.titleExtraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage99pt_PT
oaire.citation.startPage89pt_PT
oaire.citation.titleMillenium - Journal of Education, Technologies, and Healthpt_PT
oaire.citation.volume2pt_PT
person.familyNameRamalhosa
person.familyNameFernandes
person.familyNamePereira
person.givenNameElsa
person.givenNameLuana
person.givenNameJosé Alberto
person.identifier.ciencia-id1A1D-FC05-A05D
person.identifier.ciencia-id1219-7C60-1311
person.identifier.ciencia-id611F-80B2-A7C1
person.identifier.orcid0000-0003-2503-9705
person.identifier.orcid0000-0001-9377-2453
person.identifier.orcid0000-0002-2260-0600
person.identifier.ridL-6798-2014
person.identifier.scopus-author-id6602978189
person.identifier.scopus-author-id57204366348
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd74be6c0-1703-4f77-bd81-e289ec981ee3
relation.isAuthorOfPublication27b7ab18-734e-473d-b117-d2f5b2f35dc6
relation.isAuthorOfPublication7932162e-a2da-4913-b00d-17babbe51857
relation.isAuthorOfPublication.latestForDiscovery27b7ab18-734e-473d-b117-d2f5b2f35dc6

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