Advisor(s)
Abstract(s)
The industrial sector is tirelessly searching for more stable and natural colouring food matrices. Considering these demands, the present research investigated the characterization of their most promising compounds in terms of anthocyanin and betalain
pigments, followed by the bioactive effect of aqueous extracts obtained from leaves of
Tradescantia zebrina Bosse (ETZ) and Alternanthera brasiliana L. (EAB), respectively, with a view to its potential application in the industry. The chemical profile of EAB and ETZ extracts was obtained by HPLC-DAD-ESI/MS. The antioxidant activity of both extracts was evaluated by
three different in vitro assays (CAA, reducing power, and DPPH), the antimicrobial activity was tested on a panel of twelve foodborne pathogenic microorganisms and the anti-inflammatory activity was analyzed in rat macrophage cells (RAW 264.7). Finally, cytotoxicity was tested in
human tumor cells (MCF-7, NCI-H460, AGS, and CaCo) and toxicity in non-tumor cells (PLP2
and VERO) by the sulforhodamine B method. Two betalain derivatives were found in EAB
samples and four anthocyanin derivatives in ETZ samples. The best promising antioxidant
activity was showed in DPPH assay (EC50 0.024±0.001 mg/mL, ETZ), followed by reducing
power (EC50 0.53±0.01 mg/mL, EAB) and CAA (inhibition: 46% EAB, 62% ETZ, 2000 μg/mL),
proving the antioxidant potential of the two extracts. The fungicidal activity of the extracts
presented values of ≤ 2 mg/mL, and Bacillus cereus was the most sensitive bacteria, compared to other studied species. Only the EAB extract showed anti-inflammatory activity (IC50 86±3
μg/mL). Promising cytotoxicity results were evidenced, namely for EAB in NCI-H460 tumor cellline (80±8 μg/mL) and for ETZ in AGS tumor cell-line (30±2 μg/mL). None of the extracts showed toxicity for the maximum concentration tested (>400 μg/mL). Thus, it was evident that these plants proved to be a promising alternative for obtaining natural coloring pigments capable of being incorporated into the food industry.
Description
Keywords
Anthocyanins Betalains Bioactive Compounds Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology
Pedagogical Context
Citation
Junior, Eleomar Pires; Oliveira, Tatiane C.G.; Pereira, Eliana; Dias, Maria Inês; Calhelha, Ricardo C.; Kostić, Marina; Soković, Marina; Ferreira, Isabel C.F.R.; Prieto Lage, Miguel A.; Caleja, Cristina; Barros, Lillian (2022). Non-conventional edible plants: Tradescantia zebrina bosse and Althernanthera brasiliana L.. In Proceedings of 2nd International Congress on Bioactive Compounds - Galoá Proceedings. Campinas
Publisher
ICBC