Forest Research Centre

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Special bioactivities of phenolics from Acacia dealbata l. with potential for dementia, diabetes and antimicrobial treatments

Publication . Paula, Vanessa B.; Pedro, Soraia I.; Campos, Maria G.; Delgado, Teresa; Estevinho, Leticia M.; Anjos, Ofélia

Some diseases still need better therapeutic approaches, including the prevention of development. Natural resources are investigated with this purpose; among them, we decided to use an invasive plant as a main strategy. This will help in two ways: screening new compounds in flowers prevents the plant from causing widespread damage by controlling the dissemination and also obtains crude material for further applications. In the present study, flower extracts from Acacia dealbata Link harvested in Portugal were studied during three stages of flowering. Phenolic compounds were evaluated using HPLC/DAD and the total phenolics as the total flavonoids content was determined. The bioactivities screened were antioxidant potential, inhibitory activities of some enzymes (acetylcholinesterase, lipase and -glucosidase) and, to complete the screening, the inhibition of microbial growth was determined against Gram-negative and Gram-positive bacteria, as well as for yeasts. The data obtained suggested that the hydroethanolic extracts gave good results for all these biological activities and varied according to the maturation status of the flowers, with the early stage being the most active, which can be related to the chalcones content. This new approach will lead to the possible control of the invasive plant and also future perspective research for therapeutic purposes.

2022Journal article

Open access

Bioactivities of Waste Cork and Phloem Fractions of Quercus cerris Bark

Publication . Şen, Ali Umut; Almeida, Daiana; Silveira, Tayse Ferreira; Pires, Tânia C.S.P.; Añibarro-Ortega, Mikel; Mandim, Filipa; Barros, Lillian; Ferreira, Isabel C.F.R.; Pereira, Helena; Fernandes, Ângela

Recently, more and more researchers have begun to consider using waste bark fractions to produce value-added biochemicals and materials, as well as for energy production. Extraction is often the first operation in biomass biorefineries. Here we obtained hydroethanolic extracts from waste cork and phloem fractions of Quercus cerris bark and analyzed them to determine their antioxidant, antimicrobial, and nitric oxide (NO) production inhibition properties and their hepatotoxicity. The antioxidant properties were investigated by ex vivo TBARSs as well as OxHLIA antioxidant assays, the antibacterial properties against Gram-positive and Gram-negative bacteria isolated from food and clinical sources, and antifungal properties against Aspergillus brasiliensis and A. fumigatus. The NO production inhibition activity was assessed in a lipopolysaccharide (LPS)-stimulated murine macrophage (RAW 264.7) cell line, and antiproliferative activities were determined against five different cell lines, including lung (NCI-H460), gastric (AGS), breast (MCF7), and colon (CaCo2) tumor cell lines, as well as a non-tumor cell line (PLP2). The hydroethanolic maceration of waste cork and phloem yielded 4.4% and 2.4% extracts, respectively. Gallic acid glucosides, phenolic acids, and ellagic acid were identified in both extracts. The waste cork and phloem extracts showed antioxidant, antimicrobial, antifungal, and antiproliferative properties but also showed hepatotoxicity in the case of waste cork. Both bark fractions varied in terms of their bioactivity, with waste cork extracts showing, in general, higher bioactivity than phloem extracts.

2024Journal article

Open access

Mediterranean woody agroecosystems in a warming and drier climate: the importance of knowledge-based management

Publication . Pinheiro, Carla; David, Teresa S.; Baptista, Paula; Guerra-Guimarães, Leonor

The Mediterranean region is often described as a climate change hotspot. Not surprisingly, climate change impact on Medieterranean Agroecosystems sustainability, biodiversity, and productivity has been receiving a lot of interest from the research community. It is reported that the frequency, intensity and duration of droughts, as well as hotter droughts, are interfering with ecosystems’ structure, composition, and functions. To minimize some of the risks of the ongoing climate change, and maintain the economic viability of the agroecosystems, management strategies and practices need to be changed/adapted at the local and regional levels. This is of fundamental importance for the design of future human societies and their relationships with other species.

2022Journal article

Open access

Exploring the Bioactive Properties of Hydroethanolic Cork Extracts of Quercus cerris and Quercus suber

Publication . Sen, Umut; Almeida, Daiana; Silveira, Tayse Ferreira; Pires, Tânia C.S.P.; Añibarro-Ortega, Mikel; Mandim, Filipa; Barros, Lillian; Ferreira, Isabel C.F.R.; Pereira, Helena; Fernandes, Ângela

The bioactive properties of underutilized corks such as Quercus cerris cork and planted Quercus suber cork in the Eastern Mediterranean are not well-known but are crucial in developing lignocellulosic biorefineries. To assess their biological potential, hydroethanolic cork extracts of Quercus cerris and Quercus suber were analyzed for phenolic composition, antioxidant, antiproliferative, antimicrobial activities, and hepatoxicity, as well as NO-production inhibition. Here, we show that a mild hydroethanolic extraction of Q. cerris and Q. suber corks yielded 3% phenolic extracts. The phenolic composition was similar in both cork extracts, with phenolic acids and ellagitannins as the primary compounds. The bioactivity of hydroethanolic cork extracts from Q. cerris surpassed that of Q. suber and showed effectiveness against all cancer cell lines tested. This first comprehensive study on the bioactivities of different corks involves detailed characterizations of phenolic compounds of cork extracts using UPLC-DAD-ESI/MSn, evaluations of the antioxidant properties with TBARS and OxHLIA methods, evaluation of antiproliferative activity against gastric (AGS), lung (NCI-H460), colon (CaCo2), and breast cancer (MCF7) cell lines, as well as evaluations of hepatotoxicity and NO-production inhibition. The findings from this study will help bolster the potential of using underutilized cork-rich barks as a valuable resource in bark-based biorefineries.

2024Journal article

Open access

Using Analytic Hierarchy Process to Assess Beekeeping Suitability in Portuguese Controlled Areas: A First Approach

Publication . Roque, Natália; Fernandez, Paulo; Silveira, Carlos; Vilas-Boas, Miguel; Anjos, Ofélia

Simple Summary Beekeeping is an activity that supports agriculture and wildlife on earth, so it is very important to provide accurate information that is able to help in increasing the profitability of beekeeping and valorize pollination services. In this paper, a new methodology is proposed to assess beekeeping potential using Geographic Information Systems (GIS) and the Analytical Hierarchy Process. The proposed tool will support decision making by selecting the best apiary locations to maximize honey production and, consequently, the risks of losing bee colonies will be reduced. The Suitability map (apiary aptitude map) allows for validating the best locations for apiaries (considering the spatial and floristic resources use), and the aptitude for installing new apiaries or moving existing ones. In this sense, the application of Multi-criteria GIS Analysis, in the context of beekeeping planning, is a useful tool for the Beekeepers Association to manage honeybee resources within the territory in order to maximize their profit, and for government agencies to implement promotional measures and policies to maximize food support needs and mitigate spared diseases.Abstract Beekeeping management is greatly influenced by spatial factors (e.g., land use/land cover, roads, or electrical energy networks), so GIS are a powerful tool to overlap and relate a variety of spatial data levels and, consequently, a very useful tool for beekeeping activity planning. This study was developed within the intervention area of three controlled zones managed by Portuguese Beekeepers Associations. The methodology, based on multi-criteria decision analysis, integrates several criteria, such as hydrographic networks, road networks, soil occupation, solar radiation, and electromagnetic radiation sources. These criteria were proposed and evaluated through online questionnaires carried out with beekeepers. Concerning the selected criteria and the respective geographical data, the most relevant were land use/land cover and water availability, with a significance of 44% and 24%, respectively. The beekeeping suitability map enabled us to evaluate the degree of compliance for the actual location of apiaries, with 60% of the apiaries being installed in high potential areas. In the context of beekeeping planning, the potential of the techniques applied seems to be an important tool for optimizing the location of apiaries and the profitability of beekeeping.

2024Journal article

Open access