Centre for Environmental and Marine Studies

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Health impact pathways related to air quality changes: testing two health risk methodologies over a local traffic case study

Publication . Silveira, Carlos; Ferreira, Joana; Miranda, Ana I.

Air pollution causes damage and imposes risks on human health, especially in cities, where the pollutant load is a major concern, although the extent of these effects is still largely unknown. Thus, taking the busiest road traffic area in Portugal as a local case study (600 m × 600 m domain, 4 m2 spatial resolution), the objective of this work was to investigate two health risk methodologies (linear and nonlinear), which were applied for estimating short-term health impacts related to daily variations of high-resolution ambient nitrogen dioxide ( NO2) concentrations modelled for winter and summer periods. Both approaches are based on the same general equation and health input metrics, differing only in the relative risk calculation. Health outcomes, translated into the total number of cases and subsequent damage costs, were compared, and their associated uncertainties and challenges for health impact modelling were addressed. Overall, for the winter and summer periods, health outcomes considering the whole simulation domain were lower using the nonlinear methodology (less 27% and 28%, respectively). Spatially, these differences are more noticeable in locations with higher NO2 and population values, where the highest health estimates were obtained. When the daily NO2 exposure was less than 6 μg.m−3, a fact that occurred in 95% of the domain cells and in both periods, relatively small differences between approaches were found. Analysing the seasonality effect, total health impacts derived from the linear and nonlinear applications were greater in summer (around 18% in both approaches). This happens due to the magnitude and spatial variability of NO2, as the other health input metrics remained constant. This exploratory research in local scale health impact assessment (HIA) demonstrated that the use of refined input data could contribute to more accurate health estimates and that the nonlinear approach is probably the most suitable for characterising air pollution episodes, thus providing important support in HIA.

2024Journal article

Open access

Quality assessment of Portuguese monofloral honeys. Physicochemical parameters as tools in botanical source differentiation

Publication . Machado, Alexandra M.; Tomás, Andreia; Russo-Almeida, Paulo; Duarte, Aida; Antunes, Marília; Vilas-Boas, Miguel; Miguel, Maria da Graça; Figueiredo, Ana Cristina

The quality evaluation and physicochemical parameters assessment of Portuguese monofloral honeys were performed. Fifty-one monofloral honeys were collected from several regions from mainland Portugal, and from the Azores islands, producer labelled as carob tree (n = 5), chestnut (n = 2), eucalyptus (n = 5), bell heather (n = 6), incense (n = 4), lavender (n = 8), orange (n = 9), rape (n = 2), raspberry (n = 2), rosemary (n = 1), sunflower (n = 3), and strawberry tree (n = 4). Pollen analysis and microbiological safety were evaluated, and the parameters such as colour index, moisture content, electrical conductivity, hydroxymethylfurfural, pH, free and total acidity, diastase activity, proline, and sugar profile were assessed for physicochemical characterization, in all 51 monofloral honeys. After melissopalynological examination, the honeys were either confirmed as monofloral, or classified as multifloral with predominance of a specific pollen type or multifloral. Microbiological analysis showed that honeys were safe for human consumption. Pairwise comparisons of physicochemical parameters, using only honey types with n ≥ 3, revealed significant differences between honey types. Despite some homogeneity in sugar profile among honeys, eucalyptus honey was significantly different in glucose, maltose and maltulose content compared to incense, orange and sunflower honeys, and also exhibited a higher isomaltose amount compared to all analyzed honeys. Electrical conductivity, colour index, free and total acidity, and diastase activity showed significant differences between the analyzed honeys, indicating that these parameters may provide an additional tool in monofloral honey identification.

2022Journal article

Open access

Chemical Characterization and Biological Properties Assessment of Euphorbia resinifera and Euphorbia officinarum Moroccan Propolis

Publication . Boutoub, Oumaima; El-Guendouz, Soukaïna; Matos, Isabel; El Ghadraoui, Lahsen; Costa, Maria Clara; Carlier, Jorge D.; Faleiro, Maria Leonor; Figueiredo, Ana Cristina; Estevinho, Leticia M.; Miguel, Maria Graça

Although the plants of the genus Euphorbia are largely exploited by therapists in Morocco, the composition and antibacterial activities of propolis from these plants are still unknown. To address this gap, this study aimed to characterize the pollen type, the volatile compounds, and the phenolic and mineral profiles of three Euphorbia propolis samples collected in Morocco and evaluate their antimicrobial activities. The minimum inhibitory concentration of the propolis samples was determined by the microdilution method, and the anti-adherence activity was evaluated by the crystal violet assay. The examination of anti-quorum-sensing proprieties was performed using the biosensor Chromobacterium violaceum CV026. Pollen analysis revealed that Euphorbia resinifera pollen dominated in the P1 sample (58%), while E. officinarum pollen dominated in the P2 and P3 samples (44%). The volatile compounds were primarily composed of monoterpene hydrocarbons, constituting 35% in P1 and 31% in P2, with alpha-pinene being the major component in both cases, at 16% in P1 and 15% in P2. Calcium (Ca) was the predominant mineral element in both E. resinifera (P1) and E. officinarum (P2 and P3) propolis samples. Higher levels of phenols, flavonoids and dihydroflavonoids were detected in the E. officinarum P2 sample. The minimum inhibitory concentration (MIC) value ranged from 50 to 450 mu L/mL against Gram-positive and Gram-negative bacteria. Euphorbia propolis displayed the ability to inhibit quorum sensing in the biosensor C. violaceum CV026 and disrupted bacterial biofilm formation, including that of resistant bacterial pathogens. In summary, the current study evidences the potential use of E. officinarum propolis (P2 and P3) to combat important features of resistant pathogenic bacteria, such as quorum sensing and biofilm formation.

2024Journal article

Open access

Volatile characterization of honey with dominance of Bupleurum spinosum pollen

Publication . Elamine, Youssef; Machado, Alexandra M.; Estevinho, Leticia M.; Lyoussi, Badiâa; Figueiredo, Ana Cristina; Miguel, Maria Graça

Moroccan honey with Bupleurum spinosum (Apiaceae/Umbelliferae) as the main pollen source is locally known as Zantaz honey. In the present work, the volatiles from 18 honeys of this type, were isolated by hydrodistillation and analysed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS), for volatiles quantification and identification. The percentage composition of the volatiles and pollen profiling was used to determine the relationship between the different samples by cluster analysis. Two poorly correlated clusters were defined in volatiles analysis. Cluster A, with 17 out of the 18 samples, included four subgroups dominated by straight-chain hydrocarbons and fatty acids in variable proportions. Cluster B included just one sample, with 1-phenyldodec-1-en-3-one as the main component. Mono- and sesquiterpene hydrocarbons, as well as oxygen-containing mono- and sesquiterpenes occurred always <2%. Two moderately correlated clusters were defined after pollen profiling cluster analysis. Pollen Cluster A included 17 samples having in common the presence of B. spinosum and Populus sp. pollen. The one sample from Cluster B was moderately correlated with Cluster A and showed the lowest percentage of B. spinosum pollen. Pollen volatiles analysis would be relevant in assessing the presence of putative pollen volatile marker compounds and the importance of pollen profile on honey volatiles.

2024Journal article

Open access

Seasonal variation in exposure to particulate matter among children attending different levels of education: comparison of two dosimetry models

Publication . Charres, Isabella; Cipoli, Yago Alonso; Furst, Leonardo; Vicente, Estela D.; Casotti Rienda, Ismael; Lazaridis, Mihalis; Feliciano, Manuel; Alves, Célia

Exposure to particulate matter (PM) has been associated with several adverse health outcomes. Studies indicate that children may be exposed to much higher concentrations of PM at school than in other environments. There exists very little data on the deposited dose of PM while children attend classes. This study was carried out in a school located near an industrial complex in Portugal and attended by children aged 3–12 years. Indoor PM10, PM2.5 and PM1 were measured over two seasons in classrooms representing different school year groups. Particle deposition fractions in the respiratory tract, as well as the deposited doses, were calculated using the Multiple-Path Particle Dosimetry (MPPD) and the Exposure Dose Model (ExDoM2). Both models were implemented assuming an 8-hour exposure scenario to represent the school day. In general, differences in PM concentrations were observed depending on room occupancy periods and season. The highest mean PM2.5 concentration was recorded in winter when the classroom was vacant (23.7 ± 20.5 μg m-3), while the highest mean PM10 level was observed in spring during school hours (61.7 ± 24.2 μg m-3). Regardless of the dosimetry model, the highest deposition of PM10 and PM2.5 was in the upper region, while the lowest was in the tracheobronchial (TB) region. The results indicate that deposited dose and deposition fraction in spring may be more harmful to pupils’ health than in winter. PM10 presented the highest doses, ranging from 54.2 to 128 μg and from 83.9 to 185 μg, according to MPPD and ExDoM2 estimates, respectively.

2024Journal article

Open access