ESA - Dissertações de Mestrado Alunos
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- Development of a functional beverage enriched with statins from natural matricesPublication . Benhassen, Yosra; Calhelha, Ricardo C.; Heleno, Sandrina A.; Ichrak, CharfiDyslipidemia is a frequent clinical disorder that is a major risk factor for cardiovascular disease. Therefore, the common therapeutics is the use of statins, the first-line medications for treating lipid diseases. These compounds, which aroused to control and treat hypercholesterolemia and are known to be hydroxymethylglutaryl-CoA reductase (HMG-CoA) inhibitors, are usually well-tolerated, but in some cases, they can be associated with undesirableeffects, like myopathy, rhabdomyolysis, hepatotoxicity, and diabetes. In the last years, several natural molecules with bioactive potential have been isolated from natural matrices, which can be included hypocholesterolemic agents such as polysaccharides, sterols, and statin-like compounds. In this work, bio residues of the highly produced mushrooms Agaricus bisporus and Pleurotus ostreatus were explored for their richness in statin-like compounds and evaluated for their hypocholesterolemic activity. For this purpose the two mushrooms were subject to a preliminary extraction of statins by ultrasound-assisted extraction (UAE) , and a HPLC-UV identification and quantification , the highest amount of statin was detected in Pleurotus ostreatus. Afterward an optimization of the ultrasound-assisted extraction (UAE), through the Response Surface Methodology (RSM) is made to maximize the yield in pravastatin, at the optimal points of pravastatin ,ergosterol was quantified in advantage by HPLC-UV . furthermore the most promising extract , was evaluated in term of toxicity using the Sulforhodamine B (SRB) assay, yet the extract haven’t shown any toxicity to the two kind of cells , also the anti-inflammatory potential was tested . The extract was also tested for hypocholesterolemic activity in CaCo2 cells. Finally the statin like enriched extract incorporated in a milk based beverage (chocolate milkshake) where it was lyophilized and subjected to nutritional profiling .the final product have shown a crude fat content higher in the control than in the functional beverage with the extract , which probably related to the fat-lowering efficiency of the bioactive molecules present in the extract of pravastatin and ergosterol.