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- Acute Effects of Nitrate-Rich Beetroot Juice on Cardiovascular and Hemodynamic Responses to Flywheel Resistance Exercise: A Randomized, Double-Blind, Placebo-Controlled Crossover TrialPublication . Primo, Mateus Chaves; Viana, Ítalo Santiago Alves; Goulart-Silva, Leonardo Silveira; Machado, Wanderson Matheus Lopes; Leite , Luciano Bernardes; Forte, Pedro; Calhelha, Ricardo C.; Monteiro, António M.; Branquinho, Luís; Silva, Sandro Fernandes; Oliveira, Claudia Eliza Patrocínio de; Moreira, Osvaldo CostaBeetroot juice is a popular nutritional resource in sports due to its ergogenic effects, promoting vasodilation, hypotension, improved energy efficiency, and reduced oxygen cost. However, its role in modulating the autonomic nervous system during strength training remains understudied. This study assessed the effects of acute nitrate-rich beetroot juice supplementation on cardiovascular and hemodynamic responses to flywheel resistance exercise. Fifteen male participants (age 22 +/- 3.64 years) from the Federal University of Vi & ccedil;osa completed a crossover, double-blind, placebo-controlled trial. Each participant consumed either 400 mg of standardized nitrate or a placebo before performing 4 sets of 8-12 repetitions at 100% of their maximum concentric strength using a leg extension exercise, with 90 s recovery intervals. Heart rate, blood pressure, oxygen saturation, and subjective perception of effort were measured after each set. Data were analyzed using SPSS 23, employing the Shapiro-Wilk normality test, t-test for related samples, and MANOVA with time and supplement factors. NO3- supplementation led to a smaller increase in systolic blood pressure (SBP) during exercise compared to the placebo and reduced diastolic blood pressure (DBP) in the last set, reflecting decreased peripheral vascular resistance. However, no significant effects were observed for heart rate, rate-pressure product, oxygen saturation, time under tension, or subjective perception of effort. These findings suggest that NO3- supplementation can offer cardiovascular benefits by attenuating blood pressure increases during strength training, highlighting its potential as a low-risk ergogenic aid for healthy young men.
- Analysis of thermal patterns in the knee: comparative study between athletes and sedentary individualsPublication . Araújo, Mirela dos Santos; Bernardes Leite, Luciano; Forte, Pedro; Malheiro, Alexandra; Januário, William Martins; Pussieldi, Guilherme de Azambuja; Coelho, Bruno de Cássio; Barreiros, Marta de Oliveira; Barros Filho, Allan Kardec Duailibe; Assis, Miller Gomes de; Navarro, Antônio Coppi; Rodrigues Junior, Jefferson Fernando Coelho; Oliveira, Mario Norberto Sevilio de; Cabido, Christian Emmanuel Torres; Pimenta, Eduardo Mendonça; Veneroso, Christiano EduardoInfrared thermography is a non-invasive technique that detects skin-emitted radiation, providing insights into body temperature and identifying thermal changes related to inflammatory or degenerative processes. This study aimed to compare knee temperatures between sedentary individuals and athletes and to evaluate differences between the anterior and posterior knee regions. A convenience sample included 32 men, 16 sedentary individuals (1.71 ± 0.05 m; 71.7 ± 11 kg; BMI: 24.5 ± 0.39 kg/m²) and 16 athletes (1.79 ± 0.06 m; 73.29 ± 11 kg; BMI: 22.84 ± 2.52 kg/m²). Temperature assessments were conducted using infrared thermography. The results showed that the posterior knee region consistently exhibited higher temperatures than the anterior region in sedentary individuals (p < .001) and athletes (p < .001). Sedentary individuals had lower knee temperatures compared to athletes, both in the posterior regions (right: p = .0006; left: p = .0035) and anterior (right: p < .0001; left: p = .0009). These findings highlight that physical training influences localised thermal responses, with athletes demonstrating higher average knee temperatures and a consistent temperature gradient between the posterior and anterior regions in both groups.
- Analyzing Breathing Patterns in the Breaststroke Technique Through Dual-Media Kinematics and Fractal DimensionPublication . Alves, Miriam; Fonseca, Pedro; Fernandes, Aléxia; Brito, André V.; Barbosa, Tiago M.; Vilas-Boas, João PauloThe most hydrodynamic swimming position occurs with the head submerged, highlighting the benefit of reduced breathing frequency for efficiency. This study aimed to characterize and compare kinematics between two breaststroke breathing patterns—breathing every cycle and breathing every two cycles—while also analyzing intra-cyclic velocity variation (dv) and fractal dimension. In the breathing every cycle pattern, each cycle included a breath. In the breathing every cycle pattern, swimmers breathed once per cycle. In the breathing every two cycles pattern, breathing occurred every second cycle, resulting in three types of cycles: breathing, non-breathing, and the breathing cycle following a non-breathing cycle. To ensure familiarity with the new breathing pattern, swimmers underwent a six-week intervention program. They then performed three maximal 25 m bouts in each breathing pattern. Kinematic data were collected using a dual-media optoelectronic system (Qualisys AB, Sweden), integrating underwater and dry-land camera recordings. The results showed minimal differences between the three cycle types. The non-breathing cycle had the shallowest and deepest head positions, the lowest horizontal head amplitude out of water, and the smallest vertical head amplitude. It also had the fastest maximum vertical velocity of the feet and maximum center of mass velocity in the swimming direction.
- Case Report: Case study of 100 consecutive IRONMAN®-distance triathlons—impact of race splits and sleep on the performance of an elite athletePublication . Knechtle, Beat; Leite , Luciano Bernardes; Forte, Pedro; Andrade, Marilia Santos; Cuk, Ivan; Nikolaidis, Pantelis Theo; Scheer, Volker; Weiss, Katja; Rosemann, ThomasLong-distance triathletes such as IRONMAN (R) and ultra-triathletes competing in longer race distances continue to extend ultra-endurance limits. While the performance of 60 IRONMAN (R)-distance triathlons in 60 days was the longest described to date, we analysed in the present case study the impact of split disciplines and recovery in one athlete completing 100 IRONMAN (R)-distance triathlons in 100 days. To date, this is the longest self-paced world record attempt for most daily IRONMAN (R)-distance triathlons.To assess the influence of each activity's duration on the total time, the cross-correlation function was calculated for swimming, cycling, running, and sleeping times. The autocorrelation function, which measures the correlation of a time series with itself at different lags, was also employed using NumPy.The moving average for swimming slightly increased in the middle of the period, stabilizing at similar to 1.43 h. Cycling displayed notable fluctuations between similar to 5.5 and 7h, with a downward trend toward the end. The moving average for running remains high, between 5.8 and 7.2 h, showing consistency over the 100 days. The moving average for total time hovered at similar to 15 h, with peaks at the beginning, and slightly declined in the final days. The cross-correlation between swimming time and total time showed relatively low values. Cycling demonstrated a stronger correlation with total time. Running also exhibited a high correlation with total time. The cross-correlation between sleep time and swimming time presented low values. In cycling, the correlation was stronger. For running, a moderate correlation was observed. The correlation with total time was also high. The autocorrelation for swimming showed high values at short lags with a gradual decrease over time. For cycling, the autocorrelation also began strong, decreasing moderately as lags increased. Running displayed high autocorrelation at short lags, indicating a daily dependency in performance, with a gradual decay over time. The total time autocorrelation was high and remained relatively elevated with increasing lags, showing consistent dependency on cumulative efforts across all activities. In a triathlete completing 100 IRONMAN (R)-distance triathlons in 100 days, cycling and running split times have a higher influence on overall times than swimming. Swimming performance is not influenced by sleep quality, whereas cycling performance is. Swimming times slowed faster over days than cycling and running times. Any athlete intending to break this record should focus on cycling and running training in the pre-event preparation.
- Classification of dementia risk in the elderly through gait analysis with machine learning algorithmsPublication . Costa, Raí Braz; Almeida, Samuel Gonçalves; Encarnação, Samuel; Schneider, André; Barbosa, Tiago M.; Teixeira, José Eduardo; Forte, Pedro; Monteiro, António M.The irreversible and progressive decline of physiological functions is known as aging. Among these changes is brain aging, which leads to cognitive decline and the onset of dementia. This directly affects memory, learning, and motor skills, reducing gait efficiency. This study aimed to investigate the feasibility of identifying and classifying the risk of dementia based on the analysis of kinematic variables related to gait in older adults using machine learning algorithms. This cross-sectional observational study examined a sample of 59 individuals aged 60 +/- 8 years, divided into two groups: 26 institutionalized older adults (GI) and 33 non-institutionalized older adults (GNI), all residing in Bragan & ccedil;a, Portugal. Gait data were collected during a 10-m walk, recorded on video, and analyzed using Kinovea software. Cognitive status was assessed using the Mini-Mental State Examination (MMSE). Python (TM) was used for statistical analysis and to develop machine learning models to classify dementia risk based on gait variables. The results showed that the algorithmic models achieved an overall accuracy of 74.6%, with the AdaBoost algorithm performing best at 83.5%. Cross-validation revealed an overall accuracy of 72%, with the Support Vector Machine (SVM) classifier achieving the highest individual performance at 80%, correctly classifying 80% of cases across different data subsets. In conclusion, gait analysis combined with machine learning algorithms demonstrated a strong relationship between gait variables and dementia, proving to be a safe and efficient technique for dementia classification. This approach offers a low-cost and accessible early identification and intervention method, with potential applications in clinical and public health settings.
- The Effect of Flywheel Resistance Training on Executive Function in Older Women: A Randomized Controlled TrialPublication . Cota, Amanda dos Reis; Pérez Bedoya, Édison Andrés; Agostinho, Pablo Augusto Garcia; Leite, Luciano Bernardes; Schneider, André; Forte, Pedro; Monteiro, António M.; Branquinho, Luís; Teixeira, José Eduardo; Oliveira, Claudia Eliza Patrocínio de; Moreira, Osvaldo Costa; Carneiro-Júnior, Miguel AraújoExecutive function, which includes inhibitory control, working memory, and cognitive flexibility, tends to decline with aging. While traditional resistance training (TRT) has shown positive effects in mitigating these declines, limited evidence is available regarding flywheel resistance training (FRT). This study aimed to evaluate and compare the effects of TRT and FRT on executive function in older women. In this randomized controlled trial (clinicaltrials.gov NCT05910632), 29 older women were allocated into two groups: TRT (n = 15) and FRT (n = 14). The intervention lasted eight weeks with two weekly sessions conducted at the Federal University of Vi & ccedil;osa. The TRT group performed exercises using machines and free weights, while the FRT group used a multi-leg isoinertial device. Executive function was assessed using the Victoria Stroop Test (inhibitory control), Digit Span Test (working memory), and Trail Making Tests A and B (cognitive flexibility). Data were analyzed using a Multivariate Analysis of Covariance (p < 0.05). Results: No significant changes were observed in inhibitory control (p = 0.350). Working memory improved significantly within both groups in forward (p = 0.002) and backward (p = 0.002) span tasks. For cognitive flexibility, Trail Making Test A showed no significant changes (p > 0.05), but Test B showed significant within-group (p = 0.030) and between-group (p = 0.020) improvements. The B-A difference was also significant (p = 0.040). Both resistance training modalities enhanced working memory and cognitive flexibility. However, FRT produced greater improvements in cognitive flexibility, suggesting potential advantages in cognitive aging interventions.
- Effectiveness of a multicomponent physical exercise program against older adults’ frailty and obesity risk during the COVID-19 pandemic: an experimental, longitudinal, and controlled study with responsiveness analysisPublication . Encarnação, Samuel; Alves, João Ribeiro; Barbosa, Tiago M.; Forte, Pedro; Leite, Luciano Bernardes; Monteiro, António M.This study assessed the effectiveness of a multicomponent exercise training against old adults' physical fragility and obesity risk during the COVID-19 pandemic. An experimental, controlled, and longitudinal study was performed in October 2021 (during the pandemic) and after the intervention in March 2023 (during the pandemic), totalizing 16-month intervention. A total of 53 elderly (37 older women and 16 older men), aged 69.2 +/- 12.4 years, participated in this study. After some dropouts, 10 participants in the experimental group (EG) and 10 participants in the control group (CG) accomplished the whole intervention and were analyzed after the post test. Bayesian statistical paired tests were applied to analyze the pre-post changes in physical fitness and body composition components. A responsiveness analysis was performed to identify individualized improvements within the follow-up period.The results demonstrated significant benefits, particularly in body composition, with improvements in BMI, VF, and %BF. Training effectively reduced waist circumference and minimized muscle mass loss. For physical frailty, improvements were noted in LLS, while CG showed worsening in UFL but unexpected improvements in static balance measures like APB and the 95% CI ellipse. In addition, training helped mitigate declines in HG strength, ULS, LLS, ULF, LLF, DB, APB, AF, MLB, and balance measures through responsiveness analysis.The multicomponent exercise training improved lower limb strength and reduced BMI, visceral fat, and body fat percentage, with responsiveness analysis showing protective effects across most variables for participants.
- Effects of High-Intensity Interval Training on Functional Fitness in Older AdultsPublication . Schneider, André; Leite, Luciano Bernardes; Santos, Fernando; Teixeira, José Eduardo; Forte, Pedro; Barbosa, Tiago M.; Monteiro, António M.The global increase in life expectancy has generated growing interest in strategies that support functional independence and quality of life among older adults. Functional fitness—including strength, mobility, flexibility, and aerobic endurance—is essential for preserving autonomy during aging. In this context, physical exercise, particularly High-Intensity Interval Training (HIIT), has gained attention for its time efficiency and physiological benefits. This randomized controlled trial aimed to evaluate the effects of a group-based HIIT program on functional fitness in older adults. Functional outcomes were assessed before, during, and after a 65-week intervention using standardized field tests, including measures of upper and lower body strength, flexibility, aerobic endurance, and agility. This study was prospectively registered at ClinicalTrials.gov (NCT07170579). Significant improvements were observed in the HIIT group across multiple domains of functional fitness compared to the control group, notably in upper body strength, lower limb flexibility, cardiorespiratory endurance, and mobility. These results suggest that HIIT is an effective and adaptable strategy for improving functional fitness in older adults, with the potential to enhance performance in daily activities and support healthy aging in community settings.
- Effects of hydrotherapy on gait control in older adults with neurological conditions: A systematic reviewPublication . Rodrigues, Zélia; Pires, Patrícia; Pires, Sónia; Gonçalves, Sara; Pires, TelmaHydrotherapy appears safe and more effective than conventional physiotherapy for improving gait control, balance, and mobility in older adults with neurological impairments. Its buoyancy and resistance effects facilitate motor re-education and confidence in movement. Integrating aquatic therapy into neurorehabilitation programs may enhance outcomes and quality of life, although long-term follow-up studies are still needed.
- Exploratory Analysis of Physiological and Biomechanical Determinants of CrossFit Benchmark Workout Performance: The Role of Sex and Training ExperiencePublication . Malheiro, Alexandra; Forte, Pedro; Rodríguez-Rosell, David; Marques, Diogo L.; Marques, Mário C.CrossFit performance is influenced by physiological, neuromuscular, and perceptual factors, yet the extent to which these determinants vary by sex or training experience in standardized CrossFit Workouts of the Day (WODs) remains unclear. This study examined whether variables such as lactate accumulation, oxygen uptake dynamics, jump performance loss, and ventilatory responses relate differently to performance when stratified by sex and expertise. Fifteen trained athletes (eight males, seven females; overall mean age 27.7 ± 4.6 years) took part. Assessments included body composition, squat (SJ) and countermovement jumps (CMJ), and maximal oxygen consumption [VO2max]. On a separate day, they performed Fran (21-15-9 thrusters and pull-ups, Rx or scaled) The prescribed (‘Rx’) version used standardized barbell loads (43 kg for men, 29 kg for women), while the scaled version involved reduced loads or pull-up modifications. Respiratory gas exchange and heart rate were continuously monitored, while blood lactate and jump performance were measured pre- and post-WOD. Workout completion time [s] was the primary outcome. Correlation heatmaps explored associations in the overall sample and by sex and expertise. Mean completion time was 422.1 ± 173.2 s (range: 200–840). Faster performance correlated with higher ventilatory responses [ΔVe, r = −0.60, p = 0.018], greater mean VO2 (r = −0.62, p = 0.014), superior jump power [CMJ pre, r = −0.65, p = 0.009], and higher post-WOD lactate [r = −0.54, p = 0.036]. Sex-stratified analyses showed that males relied on ventilatory efficiency and neuromuscular power, whereas females were more constrained by performance loss and higher resting perceived exertion (RPE). Experts depended on ventilatory and neuromuscular efficiency, while initiates showed stronger associations with decrements in jump performance and higher RPE. These findings highlight subgroup-specific performance profiles and reinforce the need for tailored training strategies in CrossFit athletes.
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