Percorrer por autor "Valle, Juliana Silveira do"
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- Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarpPublication . Bertéli, Míria B.D.; Barros, Lillian; Reis, Filipa S.; Ferreira, Isabel C.F.R.; Glamočlija, Jasmina; Soković, Marina; Valle, Juliana Silveira do; Linde, Giani Andrea; Ruiz, Suelen Pereira; Colauto, Nelson BarrosLentinus crinitus (L.) Fr. (Basidiomycota: Polyporales) is a wild mushroom with several biotechnological applications; however, there are few studies on its chemical composition and antimicrobial activity. Therefore, this study aims to evaluate the chemical composition, cytotoxicity, and antimicrobial activity of L. crinitus basidiocarp. For that, its nutritional value (AOAC procedures) and its composition in some hydrophilic and lipophilic compounds (chromatographic techniques) were assessed. Moreover, the potential hepatotoxic effects were evaluated using a primary cell culture obtained from porcine liver, and its growth inhibitory capacity was also evaluated against four human tumour cell lines (spectrophotometric assays). The antimicrobial activity was evaluated by microdilution against eight bacteria and fungi. The basidiocarp has a high content of carbohydrates and, therefore, a relatively high energetic value. It is also rich in soluble sugars, β-tocopherol, phenolic acids, mainly p-hydroxybenzoic acid, and organic acids, mainly malic acid. L. crinitus did not show cytotoxicity in non-tumour cells, but it did not inhibit the growth of human tumour cell lines either. The basidiocarp has a wide antimicrobial activity, inhibiting the growth of different species of bacteria and fungi. It showed minimum bactericidal and fungicidal concentration values similar to or lower than those verified by commercial antibiotics or food additives used as preservatives. The antimicrobial activity was more evident against Listeria monocytogenes, Salmonella enterica, and Penicillium ochrochloron, followed by Aspergillus ochraceus and Trichoderma viride, when compared to the controls. The results obtained in this study showed that L. crinitus basidiocarp has great potential to be used by the industry without toxicity risks.
- Antioxidant Activity, Antiproliferative Activity, Antiviral Activity, NO Production Inhibition, and Chemical Composition of Essential Oils and Crude Extracts of Leaves, Flower Buds, and Stems of Tetradenia ripariaPublication . Sena, Jéssica da Silva; Rodrigues, Selma Alves; Sakumoto, Karina; Inumaro, Rodrigo Sadao; González-Maldonado, Pamela; Mendez-Scolari, Emilio; Piau, Ranulfo; Gonçalves, Daniela Dib; Mandim, Filipa; Vaz, Josiana A.; Gonçalves, José Eduardo; Sotelo, Pablo Hernan; Valle, Juliana Silveira do; Gazim, Zilda CristianiThe chemical composition of extracts (CEs) and essential oils (EOs) from Tetradenia riparia leaves, flower buds, and stems was analyzed. Antiproliferative activity against tumor cell lines, NO production inhibition, and antioxidant and antiviral activities were assessed. The CEs contained flavonoids, phenolic acids, coumarins, and saturated fatty acids. The EOs included monoterpenes, oxygenated sesquiterpenes, and diterpenes. NO production inhibition ranged from 76 to 247 μg mL−1, and antiproliferative activity exhibited GI50 between 20 and >204 μg mL−1, with low cytotoxicity (SI: 1.08 to 4.75). Reactive oxygen species inhibition ranged from 45 to 82%. Antioxidant activity varied when determined by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay (IC50: 0.51 to 8.47 mg mL−1) and ferric reducing antioxidant power (0.35 to 0.81 μM ferrous sulfate per mg). The reduction in β-carotene–linoleic acid co-oxidation varied between 76.13 and 102.25%. The total phenolic content of CEs and EOs was 10.70 to 111.68 μg gallic acid mg−1. Antiviral activity against herpes simplex virus type 1 (HSV-1) showed an EC50 between 9.64 and 24.55 μg mL−1 and an SI between 8.67 and 15.04. Leaf EOs exhibited an EC50 of 9.64 μg mL−1 and an SI of 15.04. Our study unveils the diverse chemical composition and multifaceted pharmacological properties of T. riparia, demonstrating its potential as a valuable source of bioactive compounds for therapeutic applications.
- Basidiocarp structures of Lentinus crinitus: an antimicrobial source against foodborne pathogens and food spoilage microorganismsPublication . Bertéli, Míria B.D.; Souza, Mariana Martins Magalhães de; Barros, Lillian; Ferreira, Isabel C.F.R.; Glamočlija, Jasmina; Soković, Marina; Dragunski, Douglas Cardoso; Valle, Juliana Silveira do; Ferreira, Ederlan de Souza; Pinto, Laise Cedraz; Souza, Carolina Oliveira de; Ruiz, Suelen Pereira; Linde, Giani Andrea; Colauto, Nelson BarrosLentinus crinitus basidiocarps are an alternative to antimicrobials, but the stipe (24% basidiocarp) is discarded even with potential antimicrobial activity. This study evaluated the antimicrobial activity of L. crinitus basidiocarp pileus and stipe extracts against foodborne pathogens and food spoilage microorganisms. Basidiocarps of L. crinitus were grown in sugarcane bagasse and rice husks and the pileus and stipe methanolic extract was analyzed by broth microdilution method for antimicrobial activity against eight bacteria and eight fungi. The minimum bactericidal concentration values for pileus and stipe ranged from 0.40 to 0.50 mg mL− 1, for streptomycin from 0.10 to 0.50 mg mL− 1, and for ampicillin from 0.40 to 1.20 mg mL− 1. The minimum fungicidal concentration values for pileus and stipe ranged from 0.06 to 0.60 mg mL− 1, for bifonazole from 0.20 to 0.25 mg mL− 1, and for ketoconazole from 0.30 to 3.50 mg mL− 1. Extracts had bacteriostatic, bactericidal, fungistatic and fungicidal activity against all microorganisms, but with greater efficiency and specificity for some microorganisms. Both pileus and stipe are promising and sustainable alternatives for use in food, agricultural, and pharmaceutical industries.
- Cellular antioxidant, anti-inflammatory, and antiproliferative activities from the flowers, leaves and fruits of Gallesia integrifolia spreng harmsPublication . Silva, Gabriela Catuzo Canonico; Machado, Mariane de Almeida; Sakumoto, Karina; Inumaro, Rodrigo Sadao; Gonçalves, José Eduardo; Mandim, Filipa; Vaz, Josiana A.; Valle, Juliana Silveira do; Faria, Maria Graciela Iecher; Ruiz, Suelen Pereira; Piau Junior, Ranulfo; Gonçalves, Daniela Dib; Gazim, Zilda CristianiGallesia integrifolia, a notable species in the Atlantic Forest, has been traditionally employed in folk medicine for treating rheumatism, asthma, and worms. This study investigated the cellular antioxidant, antiproliferative, and anti-inflammatory activities of the essential oils (EOs) and crude extracts (CEs) from G. integrifolia flowers, fruits, and leaves. The chemical identification of EOs was performed by GC-MS and CEs by UHPLC-MS. Cellular antioxidant and anti-inflammatory activities were assessed through mouse macrophage cell culture. In addition, the antiproliferative potential was evaluated in gastric, colorectal, breast, and lung tumor cell lines and non-tumor VERO cells. EOs predominantly contained organosulfur compounds in flowers (96.29%), fruits (94.94%), and leaves (90.72%). We found the main compound is 2,2 & PRIME;-Disulfanediyldiethanethiol in the EOs of flowers (47.00%), leaves (41.82%), and fruits (44.39%). Phenolic compounds were identified in CEs. The EOs and CEs demonstrated potential against the tumor cell lines tested (GI(50) between 51 and 230 & mu;g/mL). The selectivity index values were greater than 1.0 (1.01 to 3.37), suggesting a relative safety profile. Moreover, the anti-inflammatory activity IC50 ranged from 36.00 to 268 & mu;g/mL, and the cellular oxidation inhibition ranged from 69% to 82%. The results suggest that oils and extracts derived from G. integrifolia have potential for use in various industrial sectors.
- Light conditions affect the growth, chemical composition, antioxidant and antimicrobial activities of the white-rot fungus Lentinus crinitus mycelial biomassPublication . Halabura, Marisangela Isabel Wietzikoski; Avelino, Katielle Vieira; Araújo, Nelma Lopes; Kassem, Adma Soraia Serea; Seixas, Flávio Augusto Vicente; Barros, Lillian; Fernandes, Ângela; Liberal, Ângela; Ivanov, Marija; Soković, Marina; Linde, Giani Andrea; Colauto, Nelson Barros; Valle, Juliana Silveira doThe mycelial biomass of basidiomycetes is a promising source of compounds and represents an alternative for industrial and biotechnological applications. Fungi use light as information and hold photoresponse mechanisms, in which sensors respond to light wavelengths and regulate various biological processes. Therefore, this study aimed to investigate the effects of blue, green, and red lights on the growth, chemical composition, and antioxidant and antimicrobial activity of Lentinus crinitus mycelial biomass. The chemical composition of the mycelial biomass was determined by chromatographic methods, antioxidant activity was analyzed by in vitro assays, and antimicrobial activity was investigated by the microdilution assay. The highest mycelial biomass yield was observed under blue-light cultivation. Many primordia arose under blue or green light, whereas the stroma was formed under red light. The presence of light altered the primary fungal metabolism, increasing the carbohydrate, tocopherol, fatty acid, and soluble sugar contents, mostly mannitol, and reducing the protein and organic acid concentrations. Cultivation under red light increased the phenol concentration. In contrast, cultivation under blue and green lights decreased phenol concentration. Benzoic and gallic acids were the main phenolic acids in the hydroalcoholic extracts, and the latter acids increased in all cultures under light, especially red light. Mycelial biomass cultivated under red light showed the highest antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ferric reducing antioxidant power (FRAP) method showed that all light wavelengths increased the antioxidant activity of mycelial biomass, with the highest value under red light. Moreover, the β-carotene/linoleic acid co-oxidation (BCLA) assay demonstrated that the antioxidant activity was affected by light cultivation. Mycelial biomass grown under all conditions exhibited antibacterial and antifungal activities. Thus, mycelial biomass cultivation of L. crinitus under light conditions may be a promising strategy for controlling the mycelial chemical composition and biomass yield.
- Pachira aquatica: biological activity and chemical composition of leaves, flowers, and seedsPublication . Rahal, Isabelle Luiz; Dias, Angelica Barbosa; Canonico, Gabriela Catuzo Silva; Vieira, Marisa Cássia de Araújo Bento; Ayob, Rhaira Fernanda Casalvara; Gonçalves, José Eduardo; Colauto, Nelson Barros; Linde, Giani Andrea; Mandim, Filipa; Vaz, Josiana A.; Barros, Lillian; Valle, Juliana Silveira do; Gazim, Zilda CristianiThe chemical composition of Pachira aquatica crude extracts flowers, leaves, and seeds was obtained by UHPLC-ESI/qTOF and GC/MS. The antiproliferative activity was evaluated against the human tumour cell lines AGS (gastric), CaCo-2 (colorectal), MCF-7 (breast), and NCI-H460 (lung). The anti-inflammatory and cellular antioxidant activities were also studied. Flavonoids, phenolic acids, coumarins, and saturated fatty acids were identified in the samples. The concentration of extracts responsible for inhibiting 50% of nitric oxide production ranged from (149 to > 400 µg mL−1). Antiproliferative activity against the tumour cell lines was: AGS (GI50 175 to > 400 µg mL−1), Caco-2 (GI50 215 to > 400 µg mL−1), MCF7 (GI50 232 to > 400 µg mL−1) and NCI-H460 (GI50 208 to > 400 µg mL−1). Cellular antioxidant activity remained between 73% to > 2000%. The selectivity index (SI) ranged from 1.00 to 2.78, indicating low antiproliferative activity.