Percorrer por autor "Silva, Franceli"
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- Avaliação sensorial de infusões com diferentes misturas de Lippia alba e Syzygium aromaticumPublication . Rodrigues, Nuno; Batista, Danielle; Pereira, J.A.; Silva, FranceliNa atualidade, existe uma tendência crescente para o consumo de infusões preparadas a partir de diferentes matérias-primas, sendo a mistura de elas uma opção frequente. Contudo, para proporcionar uma maior satisfação a quem as ingere, as quantidades e proporções das misturas deve previamente ser estudada do ponto de vista sensorial. Assim, o presente trabalho teve como principal objetivo proceder à avaliação sensorial infusões obtidas a partir de diferentes misturas de lipia (Lippia albaj e de cravo (Syzygium aromaticum) nomeadamente: Infusão l (li): 25% de cravo e 75% de lipia; Infusão Zfl;): 50% de cravo e 50% de lipia; Infusão 3 (la): 75% de cravo e 25% de lipia; Infusão 4 (14): 100% de cravo; e Infusão 5 (Is): 100% de lipia, para verificar a que apresenta maior aceitação. As infusões foram preparadas à razão de lOg de mistura de plantas/L de água, com água a 80^C e deixadas a repousar durante 5 minutos. Posteriormente foram sujeitas à avaliação sensorial por um painel de doze provadores previamente treinado e que avaliou os seguintes aspetos: intensidade de cor, transparência, sensação do aroma, intensidade do aroma, sensação bucal, persistência, amargar, adstringência, doce o picante, numa escala não estruturada de O a 10. De acordo com os resultados obtidos, verificou-se que a intensidade de cor variou de 0,7 e 4,3 com os valores superiores a serem registados em lie l;. A transparência, com valores a oscilar entre 0,8 e 2,7 sendo mais elevada em l;. A 13 foi a que apresentou um aroma mais agradável comparativamente às restantes infusões com uma mediana de 7, 1, em oposição a Is foi a infusão considerada menos agradável de todas as testadas pelo painel. Na avaliação da intensidade de aroma, a [4 foi a que apresentou maior intensidade de aroma com valores médios de 7e a Isa que apresentou menores valores de intensidade de aroma com 4 de mediana. As l; e [3 apresentaram uma sensação bucal mais agradável, eas lie Iz a maior persistência. Na sensação de amargo, as l; e [4 foram as consideradas mais amargas e adstringentes. No que respeita ao doce, a maior intensidade foi detetada em l; e Ig, enquanto a menor correspondeu à 14. No picante, a [4 foi a que apresentou a maior intensidade de picante foi apresentada pela [4 e a menor pela \s. Os aromas sensoriais predominantemente referidos foram o herbal com sensação eucalipto, menta, pinho e erva cortada; aromas doces a mel néctar e aromas cítricos a limão, o que estará relacionado com a composição química dos óleos essenciais das plantas em mistura, como por exemplo o teor em eugenol do cravo que origina sensações de adocicado e o cravacol da lipia como adstringente. De uma maneira geral, a mistura que continha 50% de lipia e 50% de cravo (l;) foi a preferida pelo painel.
- Changes in volatile compounds of Dittrichia viscosa caused by the attack of the gall-forming dipteran Myopites stylatusPublication . Santos, Sónia A.P.; Mota, Luís; Malheiro, Ricardo; Silva, Franceli; Campos, Mercedes; Pinho, P. Guedes de; Pereira, J.A.The false yellowhead Dittrichia viscosa (L.) Aiton (Asteraceae) is a spontaneous plant occurring in south-ern Europe. A tritrophic relationship is observed in the aerial parts of this plant since flowers are attackedby the gall-forming dipteran Myopites stylatus (Fabricius) which, in turn, is parasitized by hymenopteranspecies. This relationship may be mediated by volatile organic compounds (VOCs) released by the aerialparts of the plant. Thus, VOCs exhaled by leaves, flowers and flower galls of D. viscosa were characterizedby headspace solid-phase microextraction (HS-SPME) and gas chromatography-ion trap-mass spectrom-etry (GC-IT-MS). A total of 46 compounds were identified, including alcohols, aldehydes, esters, ketones,phenol derivatives, terpenes and sesquiterpenes. Volatile fractions registered from the different aerialparts of D. viscosa were quantitative and qualitatively different. Leaves were composed by 31 volatile com-pounds, flowers by 34 and galls by 29. Sesquiterpenes represented about 76.8% of the volatiles exhaledfrom leaves; flowers were composed mainly by sesquiterpenes (49.1%) and terpenes (34.6%) such as -pinene, limonene and -pinene and flower galls exhaled phenol derivatives (68.7%) such as anethol andestragole, and terpenes (25.1%) such as eucalyptol and limonene. Several sesquiterpene like compoundswere exclusive from leaves, while esters (e.g. methyl-2-propylhexanoate) were exclusive from flowersand the terpene eucalyptol from flower galls. The potential effect of volatiles exhaled by the differentaerial parts of D. viscosa on the behaviour of insects implicated in the relationship with D. viscosa isdiscussed.
- Croton argyrophyllus Kunth and Croton heliotropiifolius Kunth: phytochemical characterization and bioactive propertiesPublication . Brito, Sara Samanta da Silva; Silva, Franceli; Malheiro, Ricardo; Baptista, Paula; Pereira, J.A.Croton heliotropiifolius Kunth and Croton argyrophyllus Kunth are endemic plant species from northeastern Brazil widely used in folk medicine and scarcely studied. In this context, the essential oils (EO's) and methanolic extracts (leaves and stalk) of both species were chemically characterized, and their antioxidant and antimicrobial activities were assessed. The chemical characterization of the EO's identified sixty components,being the major ones in C. argyrophyllus bicyclogermacrene (14.0%), β-pinene (8.9%) and spathulenol (8.7%), and in C. heliotropiifolius limonene (16.9%), α-pinene (13.3%) and caryophyllene (12.1%). Essential oils and methanolic extracts from leaves of C. argyrophyllus possess greater antioxidant potential, which could be related to the high levels of total phenols and flavonols. The antimicrobial activity of C. argyrophyllus essential oil proven to be more efficient than chloramphenicol (30 μg mL−1), with a minimum inhibitory concentration (MIC) of 25 μL mL−1 against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa and 10 μL mL−1 against B. cereus. Leaf extracts presented high activity against yeasts (MIC = 50 mg mL−1) being C. heliotropiifolius effective against Candida albicans and C. parapsilosis, while C. argyrophyllus was effective against C. glabrata. Overall results showed that these plant species are potential sources of phytochemicals with interest in the fields of both pharmacology (e.g., antimicrobial) and human health (e.g., antioxidant). Furthermore, in the socio-economic aspect, these results can improve and disseminate the cultivation of these species, inducing improvements in the rural populations.
- Effect of geographical origin on the essential oil content and composition of fresh and dried Mentha×villosa Hudson leavesPublication . Teles, Simone; Pereira, J.A.; Santos, Carlos H.B.; Menezes, Renata V.; Malheiro, Ricardo; Lucchese, Angélica M.; Silva, FranceliIn the present work, the effect of the geographical origin on the mass production, and essential oil content and composition of fresh and dry leaves of spearmint (Mentha ×villosa Huds.) was evaluated. Field experiments were conducted in three different municipalities (Amargosa, Cruz das Almas and Santo António de Jesus) from Bahia region (Brazil) and two drying methodologies (natural and artificial with controlled conditions) were assessed. Fresh and dried leaves essential oils were extracted by hydro-distillation and their volatiles were determined by gas chromatography with a flame ionization detector (GC/FID) and by gas chromatography with a mass selective detector (GC/MS). The results showed that Cruz das Almas field reported higher biomass production and Santo Antônio de Jesus higher leaves essential oil content. The natural and artificial drying methodologies affected in a similar way the leaves essential oil content in each region, but they affected considerably their composition. Eighteen volatile constituents were identified, mainly sesquiterpenes and monoterpenes, where piperitenone oxide and germacrene D are in higher abundance. By applying a PCA it was possible to distinguish the fresh leaves from each region from the leaves dried by the different drying methodologies, highlighting the increase of minor compounds, mainly sesquiterpenes in the dried leaves from Santo Antônio de Jesus.
- Geographical origin and drying methodology may affect the essential oil of Lippia alba (Mill) N.E. BrownPublication . Teles, Simone; Pereira, J.A.; Santos, Carlos H.B.; Menezes, Renata V.; Malheiro, Ricardo; Lucchese, Angélica M.; Silva, FranceliLippia alba (Mill.) N.E. Brown, the ginger grass, is a medicinal and aromatic plant widely used in Latin America. In this work the effect of the geographical origin and different drying methodologies on L. alba leaves production, essential oil content and composition were evaluated. Field experiments were conducted in three different municipalities (Amargosa, Cruz das Almas and Santo Antônio de Jesus) from Bahia region (Brazil) and two drying methodologies (traditional and artificial with controlled conditions) were assessed. Fresh and dried leaves essential oils were extracted by hydro-distillation and their volatiles were determined by gas chromatography.The geographical origin affected the mass production, essential oil content and both qualitative and quantitative fractions of their composition. Leaves from Cruz das Almas had higher yield in essential oil content. Overall, 26 oil constituents were identified. Carvone, germacrene D and limonene were the main oil constituents. The drying methodologies combined with the geographic origin influenced essential oil composition showing that L. alba constituents were influenced by post-harvest treatments. Some constituents were sensitive to both methods applied, while some enhanced their content like germacrene D.
- Homeopathic drugs to control red rot disease in sisal plantsPublication . Gama, Erasto V.S.; Silva, Franceli; Santos, Ivanete; Malheiro, Ricardo; Soares, Ana Cristina Fermino; Pereira, J.A.; Armond, CintiaSisal—Agave sisalana Perrine ex. Engelm—is a key economical and social crop in the semi-arid region of Brazil, especially in the Bahia state. Sisal is severely affected by Aspergillus niger Thieg, the causal agent of sisal red rot. Currently, existing control strategies are fruitless. Here, we tested different homeopathic drugs Carbo vegetabilis, Ferrum metallicum, Natrum muriaticum, phosphorus and sulphur at centesimal Hahnemannian (CH) dynamizations, of 3CH, 5CH, 7CH, 9CH and 12CH, on Aspergillus niger. We measured in vitro growth inhibition, sporulation and germination of Aspergillus niger during 12 days. We assessed in planta Aspergillus niger incidence and disease severity. Our results show that homeopathic drugs inhibited Aspergillus niger growth in vitro, in a dynamization-dependent way. Natrum muriaticum 5CH revealed the higher inhibition of 66 %, whereas sulphur 5CH yielded the lowest inhibition of 6.4 %. Spore production was stimulated using all homeopathic drugs at all dynamization levels, whereas spore germination was reduced. In planta assays revealed different results from in vitro assays, corroborating that in planta and in vitro effectiveness is different according to the homeopathic drugs applied. Here, disease incidence was not reduced significantly, but disease severity was lower in treated plants, of 27.8 % using Ferrum metallicum 9CH than in control trials, of 73.6 %. Overall, our findings suggest that homeopathic drugs could be a good strategy to control Aspergillus niger incidence in sisal plants.
- Lippia origanoides H.B.K. essential oil production, composition, and antioxidant activity under organic and mineral fertilization: effect of harvest momentPublication . Teles, Simone; Pereira, J.A.; Muniz de Oliveira, Lenaldo; Malheiro, Ricardo; Lucchese, Angélica M.; Silva, FrancelitEssential oils (EOs) chemical profile is behind a series of bioactive properties highly valued in diversifiedindustrial sectors. Strategies and control of factors affecting EO’s composition are very important andneeded.In this work, the chemical composition, antioxidant activity and production of EO’s from Lippia orig-anoides H.B.K. plants grown under organic and mineral fertilization, and harvested at different plantgrowth stages (T150, T240, T330, T420), were studied.Results demonstrate that EO’s production and composition is not affected by fertilization, both organicand mineral. Nevertheless, plant growth stage influences considerably EO’s composition and antioxidantactivity, reducing EO production. EO’s from grown plants displayed lower antioxidant activity than youngplants. Organic fertilization also displayed better antioxidant potential than EO obtained from mineralfertilization.In order to improve the potentialities and properties of L. origanoides EO’s, plants should be harvestedstill young and organic fertilizers could be applied to improve bioactivity.
- Organic and mineral fertilization influence on biomass and essential oil production, composition and antioxidant activity of Lippia origanoides HBKPublication . Teles, Simone; Pereira, J.A.; Oliveira, Lenaldo Muniz de; Malheiro, Ricardo; Machado, Serly Santigo; Lucchese, Angélica M.; Silva, FrancelitIn the present work, the effect of organic and mineral fertilization were evaluated on biomass produc-tion, essential oil content, essential oil composition, and antioxidant activity in comparison with plantscultivated without fertilization.Field evaluations reported that fertilization does not affect biomass and essential oil production of L.origanoides plants. However some variations were observed in the essential oils composition. Carvacrolwas the most abundant component followed by p-cymene, -terpinene, -caryophyllene, and thymol.Changes observed in the antioxidant potential of the different essential oils may be related to essential oils(EO’s) compositions. Essential oils from organic and mineral fertilization reported, respectively, higherand lower antiradical activity, while no differences were observed in the reducing power of the differenttreatments. Nevertheless, it was possible to partially discriminate the different fertilization treatmentsapplied thanks to the EO’s chemical composition and the bioactive potential.We conclude that for biomass and essential oil production, fertilization plans are not needed in L.origanoides plants at the applied conditions, however if it is necessary to enhance the bioactivity of theessential oils from L. origanoides, organic fertilization is encouraged.
- The temporal and spatial variation of arthropod associations inhabiting non-crop vegetation in a sisal crop, agave sisalana in the caatinga biomePublication . Brito, Sara Samanta da Silva; Villa, Maria; Benhadi-Marín, Jacinto; Silva, Franceli; Pereira, J.A.Sisal, Agave sisalana Perrine, is cultivated for fiber production, with Brazil being its leading producer. Nowadays, given the increasing interest in organic products, the market for sisal could become an economical alternative for rural areas with low economic inputs. However, sisal is threatened by different pests and diseases. Conservation biological control could contribute to the limitation of these plant enemies, but this agroecosystem is poorly known. In this context, we aimed: (i) to identify the diversity of plants and arthropods and their potential relations, (ii) to study the spatial patterns of arthropods and plants in function of the proximity to the margin of the field, and (iii) to determine the minimum sampling effort needed to record the occurring biodiversity in a sisal crop. Arthropods were sampled using pit-fall traps located close to the border and in the inner plant of the sisal crop from June to September. Simultaneously, plant species and their abundance in quadrats next to each pitfall were recorded. Diversity indexes were calculated to describe the biodiversity, a redundancy analysis was performed to analyze relations among arthropods and plants and the spatial distribution was evaluated using the non-parametric Wilcoxon rank-sum test. The redundancy analysis and the Wilcoxon test revealed a temporal and spatial distribution of arthropods and plants during the period of study. Results indicated (i) similar temporal diversity patterns from June to July for both plants and arthropods, with a maximum in July, whereas in September the biodiversity increased for arthropods and decreased for plants; (ii) the importance of particular plant species for Collembola; and (iii) that arthropods seem to colonize the sisal crop from the fields beyond the crop during the rainy season. These results provide new information about arthropods and plant biodiversity from an agroecosystem in a semi-arid region and raise further queries about the management of sisal crops.