Percorrer por autor "Santos, Priscila Dayane Freitas dos"
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- Influence of nanoencapsulated lutein on acetylcholinesterase activity: In vitro determination, kinetic parameters, and in silico docking simulationsPublication . Miranda, Cristiane Grella; Santos, Priscila Dayane Freitas dos; Silva, Jéssica Thaís do Prado; Leimann, Fernanda Vitória; Borges, Bianca Ferreira; Abreu, Rui M.V.; Ineu, Rafael P.; Gonçalves, Odinei HessLutein is a bioactive found in dark leafy vegetables that may be used as a nutraceutical agent in foodstuff and an inhibitor of key enzymes of the human body such as those involved in the cholinergic system. However, its high hydrophobicity leads to low bioavailability and must be overcome if lutein is to be added in foods. The objective of this study was to evaluate the influence of nanoencapsulated lutein in the activity of the acetylcholinesterase enzyme. The in vitro study was carried out using water in order to evaluate the impact of encapsulation on the hydrophilicity of lutein. In vitro assays showed that lutein, both free and nanoencapsulated, presented a mixedtype inhibition behavior, and encapsulated lutein was able to inhibit acetylcholinesterase activity even in an aqueous medium. Inhibition was also showed by the in silico docking results which show that lutein interacted with the pocket region of the enzyme.
- Nanoencapsulation of Curcuma longa L. extract for the treatment of experimental colitisPublication . Luz, Débora dos Anjos Weber; Rampazzo, Ana Paula de Santi; Borges, Stephanie Carvalho; Neves, Camila Quaglio; Santos, Priscila Dayane Freitas dos; Buttow, Nilza Cristina; Pires, Cassiano; Freitas, Rilton Alves de; Amado, Ciomar Aparecida Bersani; Gonçalves, Odinei Hess; Natali, Maria Raquel MarçalInflammatory bowel diseases (IBDs) are gastrointestinal chronic inflammatory diseases characterized by chronic and recurrent intestinal ulcerations. Curcuminoids are naturally occurring polyphenolic compounds with remarkable bioactivity; however, their low water affinity and bioavailability usually hinder their application. Encapsulation techniques can be coupled with extraction procedures to yield nanoparticles that may have enhanced properties. In this work, curcuminoids were extracted from rhizomes of Curcuma longa L. and simultaneously encapsulated using ultrasound. The characterization of the curcuminoids nanoparticles showed that particles ranging from 124 ± 81 to 252 ± 68 nm were formed, and the main properties of the curcuminoids were maintained after encapsulation. Experimental colitis was induced in rats by 2,4,6-trinitrobenzene sulfonic acid, and they were subsequently treated with 10, 25, or 50 mg kg-1 of nanoencapsulated curcuminoids for 7 days through gavage, during which the disease activity index (DAI) was evaluated. Then the rats were euthanized, and the distal colon was removed for macroscopic analysis and the assessment of the activity of the enzymes myeloperoxidase and N-acetylglucosaminidase. The dose of 25 mg kg-1 of nanoencapsulated curcuminoids reduced the DAI and the activity of inflammatory enzymes compared to the untreated group and was established as the minimum effective dose to be used in the treatment of this model of experimental colitis in rats.
- The nanoencapsulation of curcuminoids extracted from: Curcuma longa L. and an evaluation of their cytotoxic, enzymatic, antioxidant and anti-inflammatory activitiesPublication . Santos, Priscila Dayane Freitas dos; Francisco, Cristhian Rafael Lopes; Coqueiro, Aline; Leimann, Fernanda Vitória; Pinela, José; Calhelha, Ricardo C.; Ineu, Rafael P.; Ferreira, Isabel C.F.R.; Bona, Evandro; Gonçalves, Odinei HessCurcumin, bisdemethoxycurcumin and demethoxycurcumin are the main curcuminoids present in Curcuma longa L. and are known for their bioactivity. However, their low water solubility results in poor bioavailability and therapeutic efficacy. This work aimed to investigate the in vitro modulation capacity on the enzymes acetylcholinesterase (AChE) and glutathione S-transferase (GST), as well as the in vitro antioxidant (OxHLIA and TBARS) and anti-inflammatory activities (RAW 264.7 test) of nanoencapsulated curcuminoids. Cytotoxicity on tumor and non-tumor cell lines was also investigated. Curcuminoid nanoparticles significantly inhibited the in vitro activity of AChE (12% inhibition at 50 μM) and GST (30% inhibition at 5 μM). They presented antioxidant activity and toxic effects against breast adenocarcinoma, lung, cervical and hepatocellular carcinoma cells when dispersed in water. Encapsulated curcuminoids exhibited bioactive properties in aqueous medium (no hydrophobic solvent added), exerting antioxidant and cytotoxic effects and acting on the cholinergic and endogenous antioxidant systems.