Browsing by Author "Sampaio, Igor Carvalho Fontes"
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- Exploring Innovative Exogenous Green Stimulus Methods for Boosting Bioprocesses: Electric, Magnetic and Ultrasound Stimulation TechniquesPublication . Sampaio, Igor Carvalho Fontes; Moura, Isabela Viana Lopes de; Crugeira, Pedro Jorge Louro; Chinalia, Fábio Alexandre; Matos, Josilene Borges Tores Lima; Jones, Cleveland Maximino; Almeida, Paulo Fernando deThis book chapter provides an overview of the innovative exogenous green stimulus methods of electric, magnetic, and ultrasound stimulation and their appli- cations in boosting bioprocesses. These non-invasive and non-toxic techniques have been shown to enhance the metabolic activity and stress response of various micro- bial systems, including bacteria, yeasts, fungi, and microalgae, leading to increased yields of valuable bioproducts and bioremediation technologies. The chapter begins with an introduction to the concept of these green stimulus and the rationale behind its use in bioprocessing. This is followed by a discussion of the different principles and mechanisms of electric, magnetic, and ultrasound stimulation, including the physical and biological effects that underpin their efficacy. Additionally, the chapter covers the application of these techniques in various bioprocesses, including micro- bial fermentation, biosynthesis of enzymes, and bioconversion of biomass. Examples are provided to illustrate the benefits of green stimulus in enhancing the production of specific bioproducts, such as biofuels, pharmaceuticals, and biopolymers. Finally, we conclude with a critical evaluation of the potential of these techniques in the bioprocessing industry, highlighting the challenges and limitations that need to be addressed to fully harness their potential.
- Oilfield carbonated produced water recycling coupled to exopolysaccharide transformation by lelliottia amnigenaPublication . Sampaio, Igor Carvalho Fontes; Ferreira, Joalene de Azevedo Santos; Crugeira, Pedro Jorge Louro; Oliveira, Ian M. da Silva; Santos, Jacson Nunes; Matos, Josilene Borges Torres Lima; Pinheiro, Antonio Luiz Barbosa; Almeida, Paulo Fernando deIt was tested the production of a viscous exopolysaccharide (EPS) by Lelliottia amnigena using different concentrations (10, 15, 25, 50 and 100%) of produced water (PW) and Dialyzed PW (DPW) at 100% concentration, with a nutrient medium based on glycerin and sucrose as carbon sources in 44 h batches. There was an increase in EPS recovery when used PW up to a concentration of 25%. The viscosity of the EPS solutions was maintained with DPW and up to 15% of PW, such a condition resulted in 9.34 gL− 1 of EPS, representing an increase of 84.9% compared to the control (P < 0.0001). The PW 15% increased the viscosity per produced liter by 114.3% (P < 0.0001). Scanning electron microscopy and Energy-dispersive X-ray spectroscopy analysis showed that EPS adsorbed mineral constituents of the substrate and its characterization by Fourier Transform Infrared Spectroscopy was similar to an external control of a commercial EPS produced by an Enterobacter A47 strain. PW at 15% becomes an alternative to increase the EPS production process and can contribute to the mitigation of environmental damage through sustainable PW management in the oilfield chain.
- Up‐recycling oil produced water as the media‐base for the production of xanthan gumPublication . Sampaio, Igor Carvalho Fontes; Crugeira, Pedro Jorge Louro; Ferreira, Joalene de Azevedo Santos; Santos, Jacson Nunes; Matos, Josilene Borges Torres Lima; Pinheiro, Antonio Luiz Barbosa; Chinalia, Fabio Alexandre; Almeida, Paulo Fernando deProduced water (PW) and crude glycerin (CG) are compounds overproduced by the oil and biodiesel industry and significant scientific efforts are being applied for properly recycling them. The aim of this research is to combine such industrial byproducts for sustaining the production of xanthan by Xanthomonas campestris. Xanthan yields and viscosity on distinct PW ratios (0, 10, 15, 25, 50, 100) and on 100% dialyzed PW (DPW) in shaker batch testing identified DPW treatment as the best approach for further bioreactor experiments. Such experiments showed a xanthan yield of 17.3 g/L within 54 h and a viscosity of 512 mPa s. Physical-chemical characterization (energy dispersive X-ray spectroscopy, scanning electron microscopy and Raman spectroscopy) showed similarities between the produced gum and the experimental control. This research shows a clear alternative for upcycling high salinity PW and CG for the generation of a valued bioproduct for the oil industry.