Browsing by Author "Ruiz, Suelen Pereira"
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- Antimicrobial activity, chemical composition and cytotoxicity of: Lentinus crinitus basidiocarpPublication . Bertéli, Míria B.D.; Barros, Lillian; Reis, Filipa S.; Ferreira, Isabel C.F.R.; Glamočlija, Jasmina; Soković, Marina; Valle, Juliana Silveira do; Linde, Giani Andrea; Ruiz, Suelen Pereira; Colauto, Nelson BarrosLentinus crinitus (L.) Fr. (Basidiomycota: Polyporales) is a wild mushroom with several biotechnological applications; however, there are few studies on its chemical composition and antimicrobial activity. Therefore, this study aims to evaluate the chemical composition, cytotoxicity, and antimicrobial activity of L. crinitus basidiocarp. For that, its nutritional value (AOAC procedures) and its composition in some hydrophilic and lipophilic compounds (chromatographic techniques) were assessed. Moreover, the potential hepatotoxic effects were evaluated using a primary cell culture obtained from porcine liver, and its growth inhibitory capacity was also evaluated against four human tumour cell lines (spectrophotometric assays). The antimicrobial activity was evaluated by microdilution against eight bacteria and fungi. The basidiocarp has a high content of carbohydrates and, therefore, a relatively high energetic value. It is also rich in soluble sugars, β-tocopherol, phenolic acids, mainly p-hydroxybenzoic acid, and organic acids, mainly malic acid. L. crinitus did not show cytotoxicity in non-tumour cells, but it did not inhibit the growth of human tumour cell lines either. The basidiocarp has a wide antimicrobial activity, inhibiting the growth of different species of bacteria and fungi. It showed minimum bactericidal and fungicidal concentration values similar to or lower than those verified by commercial antibiotics or food additives used as preservatives. The antimicrobial activity was more evident against Listeria monocytogenes, Salmonella enterica, and Penicillium ochrochloron, followed by Aspergillus ochraceus and Trichoderma viride, when compared to the controls. The results obtained in this study showed that L. crinitus basidiocarp has great potential to be used by the industry without toxicity risks.
- Basidiocarp structures of Lentinus crinitus: an antimicrobial source against foodborne pathogens and food spoilage microorganismsPublication . Bertéli, Míria B.D.; Souza, Mariana Martins Magalhães de; Barros, Lillian; Ferreira, Isabel C.F.R.; Glamočlija, Jasmina; Soković, Marina; Dragunski, Douglas Cardoso; Valle, Juliana Silveira do; Ferreira, Ederlan de Souza; Pinto, Laise Cedraz; Souza, Carolina Oliveira de; Ruiz, Suelen Pereira; Linde, Giani Andrea; Colauto, Nelson BarrosLentinus crinitus basidiocarps are an alternative to antimicrobials, but the stipe (24% basidiocarp) is discarded even with potential antimicrobial activity. This study evaluated the antimicrobial activity of L. crinitus basidiocarp pileus and stipe extracts against foodborne pathogens and food spoilage microorganisms. Basidiocarps of L. crinitus were grown in sugarcane bagasse and rice husks and the pileus and stipe methanolic extract was analyzed by broth microdilution method for antimicrobial activity against eight bacteria and eight fungi. The minimum bactericidal concentration values for pileus and stipe ranged from 0.40 to 0.50 mg mL− 1, for streptomycin from 0.10 to 0.50 mg mL− 1, and for ampicillin from 0.40 to 1.20 mg mL− 1. The minimum fungicidal concentration values for pileus and stipe ranged from 0.06 to 0.60 mg mL− 1, for bifonazole from 0.20 to 0.25 mg mL− 1, and for ketoconazole from 0.30 to 3.50 mg mL− 1. Extracts had bacteriostatic, bactericidal, fungistatic and fungicidal activity against all microorganisms, but with greater efficiency and specificity for some microorganisms. Both pileus and stipe are promising and sustainable alternatives for use in food, agricultural, and pharmaceutical industries.
- Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobaccoPublication . Medeiros, Jaqueline Pavelegini de; Rodrigues, Selma Alves; Sakumoto, Karina; Ruiz, Suelen Pereira; Faria, Maria Graciela Iecher; Gonçalves, José Eduardo; Piau Junior, Ranulfo; Glamočlija, Jasmina; Soković, Marina; Gonçalves, Daniela Dib; Mandim, Filipa; Barros, Lillian; Gazim, Zilda CristianiLung cancer is the most commonly diagnosed and the main cause of cancer death, usually related to cigarette smoking. Furthermore, the microbiota of people exposed to cigarette smoke can be modified, making it difficult to eliminate opportunistic microorganisms. The leaves of Eugenia pyriformis are a by-product of fruit production and, to date, there have been no studies addressing the antiproliferative, anti-inflammatory, and antimicrobial activities. Objective Investigate the antimicrobial, Nitric Oxide (NO)-production inhibition, and antiproliferative activities of the essential oil from E. pyriformis leaves and its possible effect on the treatment and prevention of damage caused by tobacco. Methods The essential oil (EO) was obtained by hydrodistillation (3 h). Its chemical composition was investigated by GC-MS. It was proposed to investigate antiproliferative activity against human tumor cell lines, namely, breast adenocarcinoma (MCF-7), lung (NCI-H460), cervical (HeLa), and hepatocellular (HepG2) carcinomas. A non-tumor primary culture from pig liver (PLP2) was also tested. The EO capacity to inhibit nitric oxide (NO) production was evaluated by a lipopolysaccharide stimulated murine macrophage cell line. Antibacterial and antifungal activities against opportunistic pathogens were investigated against seven strains of bacteria and eight fungi. Results The results indicated the presence of 23 compounds in the essential oil, the majority were spathulenol (45.63%) and beta-caryophyllene oxide (12.72%). Leaf EO provided 50% inhibition of nitric oxide production at a concentration of 92.04 mu g mL(-1). The EO also demonstrated antiproliferative activity against all human tumor cell lines studied, with GI50 values comprised between 270.86 and 337.25 mu g mL(-1). The essential oil showed antimicrobial potential against the bacteria Listeria monocytogenes (Murray et al.) Pirie (NCTC 7973) and Salmonella Typhimurium ATCC 13311 (MIC 1870 mu g mL(-1)) and fungi Aspergillus versicolor ATCC 11730, Aspergillus ochraceus ATCC 12066, Penicillium ochrochloron ATCC 90288, Penicillium verrucosum var. cyclopium (Westling) Samson, Stolk & Hadlok (food isolate) (MIC 1870 mu g mL(-1)) and Trichoderma viride Pers. IAM 5061 (1,400 mu g mL(-1)). Conclusion The demonstrated anti-inflammatory, antiproliferative, and antimicrobial activities in the leaves of E. pyriformis can add value to the production chain of this plant, being a possible option for preventing and combating cancer, including lung cancer.
- Cellular antioxidant, anti-inflammatory, and antiproliferative activities from the flowers, leaves and fruits of Gallesia integrifolia spreng harmsPublication . Silva, Gabriela Catuzo Canonico; Machado, Mariane de Almeida; Sakumoto, Karina; Inumaro, Rodrigo Sadao; Gonçalves, José Eduardo; Mandim, Filipa; Vaz, Josiana A.; Valle, Juliana Silveira do; Faria, Maria Graciela Iecher; Ruiz, Suelen Pereira; Piau Junior, Ranulfo; Gonçalves, Daniela Dib; Gazim, Zilda CristianiGallesia integrifolia, a notable species in the Atlantic Forest, has been traditionally employed in folk medicine for treating rheumatism, asthma, and worms. This study investigated the cellular antioxidant, antiproliferative, and anti-inflammatory activities of the essential oils (EOs) and crude extracts (CEs) from G. integrifolia flowers, fruits, and leaves. The chemical identification of EOs was performed by GC-MS and CEs by UHPLC-MS. Cellular antioxidant and anti-inflammatory activities were assessed through mouse macrophage cell culture. In addition, the antiproliferative potential was evaluated in gastric, colorectal, breast, and lung tumor cell lines and non-tumor VERO cells. EOs predominantly contained organosulfur compounds in flowers (96.29%), fruits (94.94%), and leaves (90.72%). We found the main compound is 2,2 & PRIME;-Disulfanediyldiethanethiol in the EOs of flowers (47.00%), leaves (41.82%), and fruits (44.39%). Phenolic compounds were identified in CEs. The EOs and CEs demonstrated potential against the tumor cell lines tested (GI(50) between 51 and 230 & mu;g/mL). The selectivity index values were greater than 1.0 (1.01 to 3.37), suggesting a relative safety profile. Moreover, the anti-inflammatory activity IC50 ranged from 36.00 to 268 & mu;g/mL, and the cellular oxidation inhibition ranged from 69% to 82%. The results suggest that oils and extracts derived from G. integrifolia have potential for use in various industrial sectors.