Percorrer por autor "Pimentel, Tatiana Colombo"
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- Chemical profile and bioactivities of industrial wastes from Chenopodium quinoa seedPublication . Casalvara, Rhaira Fernanda Ayoub; Santos, Everton da Silva; Mattos, Jose Vinicius; Pimentel, Tatiana Colombo; Calhelha, Ricardo C.; Pires, Tânia C.S.P.; Rodrigues, Daniele Bobrowski; Pereira, Carla; Corrêa, Rúbia C.G.; Cardozo‐Filho, Lucio; Gonçalves, José EduardoThis study aimed to investigate the effect of extraction methods and solvents (Soxhlet and supercritical fluid extraction, SFE) on the chemical profile (gas chromatography [GC]/mass spectrometry and GC/flame ionization detection) and antimicrobial, antioxidant, antitumor, and anti-inflammatory activities of quinoa Piabiru (QP) husk crude extracts. Soxhlet was applied using 100% water (QPSH2O), and ethanolic solutions of 50% ethanol (ethanol:water 50:50 v/v, QPSetOH50), 70% ethanol (QPSetOH70), and 99% ethanol (QPSetOH99) as solvents. SFE was applied using CO2 and n-propane as solvents (QPSF). QPSH2O extract showed a higher concentration of phytosterols (stigmasterol, β-sitosterol, 7,8-epoxylanostan-11-OL,3-acetoxy) and carotenoids (rhodopin) and oleic acid, displaying intermediate thiobarbituric acid reactive substance (TBARS) antioxidant activity. Intermediate concentrations of ethanol (QPSetOH50 and QPSetOH70 extracts) increased the extraction yields and the antibacterial activity of the extracts (Pseudomonas aeruginosa, Salmonella enterica, Bacillus cereus, and Staphylococcus aureus). Higher concentrations of ethanol (QPSetOH99 extract) contributed to increased antioxidant activity as assessed by TBARS and higher recoveries of 4-(allyloxy)-2-methyl-2-pentanol, nonadecane, and lauric, myristic, palmitic, linoleic, stearic, arachidic, behenic, and lignoceric acids. Finally, the QPSF extract presented higher antioxidant activity by DPPH, ABTS, and ferric-reducing antioxidant power, higher content of 5-methoxy-2-pentanone, 5-methoxy-2-methyl-2-pentanol, 1-(1,3-dimethylbutoxy)-2-propanol, oxalic, undecanoic, myristoleic, tricosanoic, pentadecanoic, elaidic, 11-eicosenoic, and erucic acids, and better antifungal activity against Aspergillus brasiliensis than the other extracts. Crude extracts were not cytotoxic against non-tumor cells (Vero) and did not show antitumor or antiinflammatory activities. Thus, antagonistic or synergistic effects of the phytochemical profile of quinoa husk crude extracts may present potential food and pharmaceutical applications. Practical Applications: The exceptional nutritional properties of quinoa seeds have boosted their cultivation in more than 123 countries. However, quinoa husks are generally considered waste. This study, which is of utmost importance, demonstrates the potential of extracting bioactive compounds from quinoa husks via pressurized fluids, turning them into a health-promoting co-product. This approach could minimize the current shortage of new antibiotics, antifungals, antitumor agents, and anti-inflammatory substances in the pharmaceutical and food sectors. By converting quinoa husks into valuable bioactive extracts,we contribute significantly to developing effective natural compounds, underlining the significance of our collective work.
- Chemical profile and bioactivities of industrial wastes from Chenopodium quinoa seedPublication . Casalvara, Rhaira Fernanda Ayoub; Santos, Everton da Silva; Mattos, Jose Vinicius; Pimentel, Tatiana Colombo; Calhelha, Ricardo C.; Pires, Tânia C.S.P.; Rodrigues, Daniele Bobrowski; Pereira, Carla; Corrêa, Rúbia C.G.; Cardozo‐Filho, Lucio; Gonçalves, José EduardoThis study aimed to investigate the effect of extraction methods and solvents (Soxhlet and supercritical fluid extraction, SFE) on the chemical profile (gas chromatography [GC]/mass spectrometry and GC/flame ionization detection) and antimicrobial, antioxidant, antitumor, and anti-inflammatory activities of quinoa Piabiru (QP) husk crude extracts. Soxhlet was applied using 100% water (QPSH2O), and ethanolic solutions of 50% ethanol (ethanol:water 50:50 v/v, QPSetOH50), 70% ethanol (QPSetOH70), and 99% ethanol (QPSetOH99) as solvents. SFE was applied using CO2 and n-propane as solvents (QPSF). QPSH2O extract showed a higher concentration of phytosterols (stigmasterol, β-sitosterol, 7,8-epoxylanostan-11-OL,3-acetoxy) and carotenoids (rhodopin) and oleic acid, displaying intermediate thiobarbituric acid reactive substance (TBARS) antioxidant activity. Intermediate concentrations of ethanol (QPSetOH50 and QPSetOH70 extracts) increased the extraction yields and the antibacterial activity of the extracts (Pseudomonas aeruginosa, Salmonella enterica, Bacillus cereus, and Staphylococcus aureus). Higher concentrations of ethanol (QPSetOH99 extract) contributed to increased antioxidant activity as assessed by TBARS and higher recoveries of 4-(allyloxy)-2-methyl-2-pentanol, nonadecane, and lauric, myristic, palmitic, linoleic, stearic, arachidic, behenic, and lignoceric acids. Finally, the QPSF extract presented higher antioxidant activity by DPPH, ABTS, and ferric-reducing antioxidant power, higher content of 5-methoxy-2-pentanone, 5-methoxy-2-methyl- 2-pentanol, 1-(1,3-dimethylbutoxy)-2-propanol, oxalic, undecanoic, myristoleic, tricosanoic, pentadecanoic, elaidic, 11-eicosenoic, and erucic acids, and better antifungal activity against Aspergillus brasiliensis than the other extracts. Crude extracts were not cytotoxic against non-tumor cells (Vero) and did not show antitumor or antiinflammatory activities. Thus, antagonistic or synergistic effects of the phytochemical profile of quinoa husk crude extracts may present potential food and pharmaceutical applications. Practical Applications: The exceptional nutritional properties of quinoa seeds have boosted their cultivation in more than 123 countries. However, quinoa husks are generally considered waste. This study, which is of utmost importance, demonstrates the potential of extracting bioactive compounds from quinoa husks via pressurized fluids, turning them into a health-promoting co-product. This approach could minimize the current shortage of new antibiotics, antifungals, antitumor agents, and anti-inflammatory substances in the pharmaceutical and food sectors. By converting quinoa husks into valuable bioactive extracts,we contribute significantly to developing effective natural compounds, underlining the significance of our collective work.
- Implementation of Sustainable Development Goals in the dairy sector: perspectives on the use of agro-industrial side-streams to design functional foodsPublication . Granato, Daniel; Carocho, Márcio; Barros, Lillian; Zabetakis, Ioannis; Mocan, Andrei; Tsoupras, Alexandros; Cruz, Adriano Gomes; Pimentel, Tatiana ColomboFrom 2017 to 2020, global milk production ranged from 610,724 to 643,769 thousand tons, but the dairy industry still faces issues related to its carbon footprint and sustainability. According to the United Nations Sustainable Development Goals (SDGs), by 2030, food processors, governmental bodies, and consumers should take actions regarding food production patterns and consumption to decrease the generation of by-products and side-streams and increase their circularity by developing nutritious-rich products. Dairy products have traditionally been manufactured without bioactive ingredients to boost consumers’ health and well-being. To achieve the sustainability goals and the need to reformulate traditional dairy foods to make them more nutritious and reduce their carbon footprint, it is paramount to implement integrated approaches that embody the “farm to fork” ethos. Scope and approach: This review integrates concepts of food science, technology, nutrition, circular economy, and sustainability to provide an overview of the technological applications of dietary fibre, polyphenols, functional lipids, and carotenoids obtained from agro-industrial side-streams in dairy food formulations. Key findings and conclusions: Dairy processors can use bioactive ingredients and extracts obtained from agroindustrial side-streams to design potentially functional food models and tentatively market these products with nutritional claims or even with a health claim in case the bioactivity is verified in human intervention trials. This approach will increase the nutritional value of traditional dairy foods and contribute to circularity within food systems, reducing food waste, and enhancing human health.
- Supercritical fluid-assisted extraction of hop cone residue from craft breweriesPublication . Inumaro, Rodrigo Sadao; Casalvara, Rhaira Fernanda Ayoub; Corrêa, Rúbia C.G.; Barros, Lillian; Pereira, Carla; Calhelha, Ricardo C.; Heleno, Sandrina A.; Pimentel, Tatiana Colombo; Gonçalves, José Eduardo; Cardozo-Filho, LucioThis study aimed to use supercritical fluid-assisted extraction in hop cone residue from craft breweries and define the best extraction parameters. Yield, compounds extracted, in vitro chemical and cell-based antioxidant ac-tivities, and antimicrobial and cytotoxic potentials were evaluated. The variables studied were temperature (40-60 degrees C), pressure (10-20 MPa), and ethanol percentage as co-solvent (5-10%) using a Taguchi experimental design (11 runs). The extracts showed inhibitory action towards Gram-positive and Gram-negative bacteria, mainly Proteus mirabilis and Enterococcus faecalis (MICs = 5-10 mg/mL). Furthermore, up to the maximum tested concentration (400 & mu;g/mL), the extracts showed no hepatotoxicity on a healthy porcine liver cell line (PLP2), indicating safety for food and pharmacological applications. Lower temperatures (40 degrees C), pressures (10 MPa), and ethanol percentages (5%) resulted in higher concentrations of some compounds (alpha-copaene, & gamma;-elemene, & gamma;-muurolene, & alpha;-muurolene, caryophyllene oxide) and higher antioxidant activity in cell-based assays, but lower yields. At the same time, lower temperatures (40 degrees C) and pressures (10 MPa) but higher ethanol percentages (10%) resulted in higher concentrations of other compounds (nerolidyl acetate, linalyl isobutyrate, & alpha;-acorenol, and geranyl isovalerate) and increased antioxidant activity in chemical systems. Our results support that the hop cone residue can be used to obtain supercritical fluid-assisted extracts with biological potentialities that might virtually be upcycled into multifunctional ingredients for the food and pharmacological industries. Using 40 degrees C, 10 MPa, and 5 or 10% ethanol percentage as process parameters is advisable.