Percorrer por autor "Lourenço-Lopes, Catarina"
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- Biological action mechanisms of fucoxanthin extracted from algae for application in food and cosmetic industriesPublication . Lourenço-Lopes, Catarina; Fraga-Corral, Maria; Jiménez López, Cecília; Carpena Rodríguez, María; González Pereira, Antía; García-Oliveira, Paula; Prieto Lage, Miguel A.; Simal-Gandara, JesusBackground: Fucoxanthin is a pigment present in diverse marine organisms such as micro and macro-algae. The most relevant source of fucoxanthin has been described to be the group of the brown macroalgae, also known as Phaeophyceae. The presence of the fucoxanthin, a xanthophyll, found as an accessory pigment in the chloroplasts of the brown algae is responsible for providing them their characteristic color. The market size of this carotenoid, expected to reach US$ 120 million by 2022, reflects its industrial importance, especially remarkable as a food or cosmetic ingredient. Scope and approach: Therefore, it is critical to recognize the main sources of fucoxanthin as well as the most efficient extraction and purification methods that allow obtaining the best production ratio for such a valuable molecule. Throughout this review very different preventive properties of the fucoxanthin have been included, such as antioxidant, anticancer, antiangiogenic, anti-inflammatory, cytoprotective, antiobesity, neuroprotective and its skin protective effects. The stability, bioavailability and toxicity of the fucoxanthin have also been reviewed through diverse biological, in vitro and in vivo assays. Key findings and conclusions: Thus, the main aim of this work is to provide a wide and global vision of the fucoxanthin in terms of productive species, efficient recovery techniques and multiple industrial applications.
- Biological properties and potential of compounds extracted from red seaweedsPublication . Carpena Rodríguez, María; García-Pérez, Pascual; García-Oliveira, Paula; Chamorro Rivo, Franklin Noel; Otero, Paz; Lourenço-Lopes, Catarina; Cao, Hui; Simal-Gandara, Jesus; Prieto Lage, Miguel A.Macroalgae have been recently used for different applications in the food, cosmetic and pharmaceutical industry since they do not compete for land and freshwater against other resources. Moreover, they have been highlighted as a potential source of bioactive compounds. Red algae (Rhodo- phyta) are the largest group of seaweeds, including around 6000 different species, thus it can be hypoth- esized that they are a potential source of bioactive compounds. Sulfated polysaccharides, mainly agar and carrageenans, are the most relevant and exploited compounds of red algae. Other potential molecules are essential fatty acids, phycobiliproteins, vitamins, minerals, and other secondary metabolites. All these compounds have been demonstrated to exert several biological activities, among which antioxidant, anti- inflammatory, antitumor, and antimicrobial properties can be highlighted. Nevertheless, these properties need to be further tested on in vivo experiments and go in-depth in the study of the mechanism of action of the specific molecules and the understanding of the structure–activity relation. At last, the extraction technologies are essential for the correct isolation of the molecules, in a cost-effective way, to facilitate the scale-up of the processes and their further application by the industry. This manuscript is aimed at describing the fundamental composition of red algae and their most studied biological properties to pave the way to the utilization of this underused resource.
- By-products of walnut (Juglans regia) as source of bioactive compounds for the formulation of nutraceuticals and functional foodsPublication . Chamorro Rivo, Franklin Noel; Carpena Rodríguez, María; Lourenço-Lopes, Catarina; Taofiq, Oludemi; Otero, Paz; Cao, Hui; Xiao, Jianbo; Simal-Gandara, Jesus; Prieto Lage, Miguel A.The scarcity of natural resources and a higher incidence of diseases related to inappropri-ate eating habits have focused research on the search for food and nutraceutical products with func-tional properties that are respectful of the environment. Agro-industrial by-products are a profitable source for obtaining bioactive compounds, with various biological properties, including antioxi-dant, anti-inflammatory, and anticancer properties, which contribute to immunity and reduce the negative effects of infections, inflammation, and oxidative stress. In the case of the walnut oil indus-try, various by-products are generated, among which we can mention pomace, green shell, shell, skin and leaves. Therefore, there is an opportunity for the recovery of waste, the recovery of target molecules and the formulation of new products, whether they are nutraceuticals, pharmaceuticals, or food additives, contributing to the circular economy and consumer health. The nut is commonly characterized by its high content of lipids (58–65%), mainly polyunsaturated fatty acids, tocopherols and phytosterols. In addition, the current literature states that its by-products are rich in phenolic compounds, mainly phenolic acids and flavonoids, for example total phenolic concentrations in the range 74 to 410 mg/g of GAE/FW are reported. In accordance, it is reported that the antioxidant potential of different extracts of nuts, shells and leaves was studied using different methods such as the reduction potency assay, scavenging effect of DPPH (2,2-diphenyl-1-picrylhydrazyl) and in-hibition of lipid oxidation by β-carotene linoleate system, with EC50 values less than 1 mg/mL. The results obtained showed that all walnut extracts have a strong antioxidant capacity against ROS species. For this reason, this work focuses on the bibliographic review of the bioactive compounds present in the by-products of the walnut (Juglans regia) industry, as well as mentioning their biolog-ical properties and possible applications in the food industry
- Evolution of flavors in extra virgin olive oil shelf-lifePublication . García-Oliveira, Paula; Jiménez López, Cecília; Lourenço-Lopes, Catarina; Chamorro Rivo, Franklin Noel; González Pereira, Antía; Carreira Casais, Anxo; Fraga-Corral, Maria; Carpena Rodríguez, María; Simal-Gandara, Jesus; Prieto Lage, Miguel A.Extra virgin olive oil (EVOO) is one of the most distinctive ingredients of the Mediterranean diet. There are many properties related to this golden ingredient, from supreme organoleptic characteristics to benefits for human health. EVOO contains in its composition molecules capable of exerting bioactivities such as cardio protection, antioxidant, anti-inflammatory, antidiabetic, and anticancer activity, among others, mainly caused by unsaturated fatty acids and certain minor compounds such as tocopherols or phenolic compounds. EVOO is considered the highest quality vegetable oil, which also implies a high sensory quality. The organoleptic properties related to the flavor of this valued product are also due to the presence of a series of compounds in its composition, mainly some carbonyl compounds found in the volatile fraction, although some minor compounds such as phenolic compounds also contribute. However, these properties are greatly affected by the incidence of certain factors, both intrinsic, such as the olive variety, and extrinsic, such as the growing conditions, so that each EVOO has a particular flavor. Furthermore, these flavors are susceptible to change under the influence of other factors throughout the oil's shelf-life, such as oxidation or temperature. This work offers a description of some of the most remarkable compounds responsible for EVOO’s unique flavor and aroma, the factors affecting them, the mechanism that lead to the degradation of EVOO, and how flavors can be altered during the shelf-life of the oil, as well as several strategies suggested for the preservation of this flavor, on which the quality of the product also depends.
- Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studiesPublication . Lourenço-Lopes, Catarina; Fraga-Corral, Maria; Soria-Lopez, Anton; Núñez Estévez, Bernabé; Barral Martínez, Marta; Silva, Aurora; Li, Ningyang; Liu, Chao; Simal-Gandara, Jesus; Prieto Lage, Miguel A.Brown macroalgae are a potential source of natural pigments. Among them, Undaria pinnatifida is recognized for its high concentration of fucoxanthin (Fx), which is a pigment with a wide range of bioactivities. In this study, three independent parameters were optimized for conventional heat extraction (CHE) to maximize the recovery of Fx from Undaria pinnatifida. Optimal conditions (temperature = 45 °C, solvent = 70%, and time = 61 min) extracted 5.1 mg Fx/g dw. Later, the bioactivities of the Fx-rich extracts (antioxidant, antimicrobial, and neuroprotective) were assessed using in vitro and in silico approaches. In vitro assays indicated that Fx has a strong antioxidant capacity and even stronger antimicrobial activity against gram-positive bacteria. This data was supported in silico where Fx established a high binding affinity to DR, a Staphylococcus aureus protein, through aa ALA-8, LEU-21, and other alkane interactions. Finally, the in vitro enzymatic inhibition of AChE using Fx, was further supported using docking models that displayed Fx as having a high affinity for aa TYR72 and THR 75; therefore, the Fx extraction behavior explored in this work may reduce the costs associated with energy and solvent consumption. Moreover, this paper demonstrates the efficiency of CHE when recovering high amounts of Fx from Undaria pinnatifida. Furthermore, these findings can be applied in different industries.
- A HPLC‐DAD method for identifying and estimating the content of fucoxanthin, β‐carotene and chlorophyll a in brown algal extractsPublication . Lourenço-Lopes, Catarina; Fraga-Corral, Maria; Garcia-Perez, Pascual; Carreira-Casais, Anxo; Silva, Aurora; Simal-Gandara, Jesus; Prieto Lage, Miguel A.Seaweeds are photosynthetic organisms that have high contents of pigments. The coloration of each alga is defined by the content and combination of pigments synthesized, which varies among species and environmental condi- tions. The most abundant pigments in algae are chlorophylls and carotenoids, lipophilic molecules that can be used as natural colorants and have high acceptance by consumers. In this work, a simple and short hands-on time HPLC-DAD method for identifying and estimating the pigment content of algal extracts, specifically fucoxanthin, 𝛽-carotene and chlorophyll a was carried out. Using this optimized method, a pigment screening was performed on the ethanolic extracts obtained by ultrasound-assisted extraction from nine brown algal from the Atlantic coastline: Ascophyllum nodosum, Bifurcaria bifurcata, Fucus spiralis, Himanthalia elongata, Laminaria saccharina, Laminaria ochroleuca, Pelvetia canaliculata, Sargassum muticum and Undaria pinnatifida . HPLC results permitted to highlight L. saccharina and U. pinnatifida as promising sources of these three target pigments containing a total amount of 10.5 –11.5 mg per gram of dry weight. Among them, the most abundant one was fucoxanthin, an added-value compound with a high potential to be commercially exploited by different industries, such as the food, cosmetic, and pharmaceutical sectors.
- Main bioactive phenolic compounds in marine algae and their mechanisms of action supporting potential health benefitsPublication . Jiménez López, Cecília; González Pereira, Antía; Lourenço-Lopes, Catarina; García-Oliveira, Paula; Cassani, Lucia; Fraga-Corral, Maria; Prieto Lage, Miguel A.; Simal-Gandara, JesusGiven the growing tendency of consumers to choose products with natural ingredients, food industries have directed scientific research in this direction. In this regard, algae are an attractive option for the research, since they can synthesize a group of secondary metabolites, called phenolic compounds, associated with really promising properties and bioactivities. The objective of this work was to classify the major phenolic compounds, compare the effectiveness of the different extractive techniques used for their extraction, from traditional systems (like heat assisted extraction) to the most advance ones (such as ultrasound, microwave or supercritical fluid extraction); the available methods for identification and quantification; the stability of the enriched extract in phenolic compounds and the main bioactivities described for these secondary metabolites, to offer an overview of the situation to consider if it is possible and/or convenient an orientation of phenolic compounds from algae towards an industrial application.
- Pigment composition of nine brown algae from the iberian northwestern coastline: influence of the extraction solventePublication . García-Pérez, Pascual; Lourenço-Lopes, Catarina; Silva, Aurora; González Pereira, Antía; Fraga-Corral, Maria; Zhao, Chao; Xiao, Jianbo; Simal-Gandara, Jesus; Prieto Lage, Miguel A.Brown algae are ubiquitously distributed in the NW coastline of the Iberian Peninsula, where they stand as an underexploited resource. In this study, five solvents were applied to the extraction of pigments from nine brown algae, followed by their determination and quantification by HPLC-DAD. A total of 13 compounds were detected: Six were identified as chlorophylls, six were classified as xanthophylls, and one compound was reported as a carotene. Fucoxanthin was reported in all extracts, which is the most prominent pigment of these algae. Among them, L. saccharina and U. pinnatifida present the highest concentration of fucoxanthin (4.5–4.7 mg g-1 dry weight). Ethanol and acetone were revealed as the most efficient solvents for the extraction of pigments, showing a maximal value of 11.9 mg of total pigments per gram of dry alga obtained from the ethanolic extracts of H. elongata, followed by the acetonic extracts of L. ochroleuca. Indeed, ethanol was also revealed as the most efficient solvent according to its high extraction yield along all species evaluated. Our results supply insights into the pigment composition of brown algae, opening new perspectives on their commercial exploitation by food, pharmaceutical, and cosmeceutical industries.
- Scientific basis for the industrialization of traditionally used plants of the Rosaceae familyPublication . García-Oliveira, Paula; Fraga-Corral, Maria; González Pereira, Antía; Lourenço-Lopes, Catarina; Jiménez López, Cecília; Prieto Lage, Miguel A.; Simal-Gandara, Jesus© 2020 Elsevier Ltd Plants have been traditionally used for the treatment of different types of illness, due to biomolecules with recognised benefits. Rosaceae family is used in traditional Galician medicine. The following plants Agrimonia eupatoria, Crataegus monogyna, Filipendula ulmaria, Geum urbanum, Potentilla erecta and Rosa canina are usually found in treatments. The aim of this study is to perform an ethnobotanical review about the bioactive compounds of these plants and their different bioactivities, both studied in vitro and in vivo. The nature of the bioactive compounds is varied, highlighting the presence of different phenolic compounds, such as phenolic acids, flavonoids or tannins. Understanding the beneficial effects of the administration of the whole plant or target tissues from A. eupatoria, C. monogyna, F. ulmaria, G. urbanum, P. erecta and R. canina as well as those from their individual compounds could lead to the development of new drugs based on the use of natural ingredients.
- The use of seaweed in daily diets as a source of nutrientesPublication . García-Oliveira, Paula; Carreira Casais, Anxo; Pereira, Eliana; Caleja, Cristina; Lourenço-Lopes, Catarina; Barros, Lillian; Pérez-Juste, Jorge; Prieto Lage, Miguel A.; Simal-Gandara, Jesus; Ferreira, Isabel C.F.R.Seaweed plays an important role in the biosphere being responsible for most of the production in aquatic ecosystems [1]. Although commonly consumed since ancient times in Asia, their consumption has been increasing in Europe and North America [2]. These species are known for their excellent nutritional value, especially as a protein sources and low lipid and caloric content. In addition, they are rich in several bioactive compounds, such as vitamins, essential fatty acids and phenolic compounds, providing important health benefits to consumers [3]. This work aims to determine ash, proteins, fat, carbohydrates and energy contents, as also the composition in free sugars, organic acids and fatty acids of some seaweeds species, namely Himanthalia elongate L., S.F. Gray; Laminaria ochroleuca Bach.Pyl.; Saccharina latissima L.; Porphyra sp. C.Agardh; Palmaria palmata (L.) Kuntze, and Undaria pinnatifida (Harvey) Suringar. The nutritional aspects were determined using official methodologies for food analysis, while free sugars were identified by HPLC-RI, organic acids by UPFLC-PDA and fatty acids by GC-FID. Proteins stood out as the main macronutrients present, with values that oscillated between 6 and 30 g/100 g of dry weight (dw) for S. latissima and Porphyra sp., respectively. On the other hand, a low lipid content was evident in all the studied seaweed species with values below 1 g/100 g dw, as well as low carbohydrates’ content. The energy values ranged from 218 and 370 kcal/100 g dw for L. ochrouleuca and Porphyra sp, respectively. Furthermore, several compounds of interest were identified, namely free sugars and organic acids, in this case being oxalic acid the only molecule present in all species. Saturated, unsaturated and polyunsaturated fatty acids were also determined, showing a clear heterogeneity of concentrations depending on the species. The present study demonstrates the high potential of these seaweed species as an alternative source of non-animal protein, as well as some molecules with bioactive properties. Thus, the incorporation of this food into the daily diet will contribute for the improvement of a number of functionalities.