Percorrer por autor "Jorge, M."
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- Experimental and molecular dynamics simulation studies on thee solubilityof amino acids in aqueous electrolyte solutionsPublication . Tomé, L.I.N.; Pinho, Simão; Jorge, M.; Gomes, J.R.B.; Coutinho, João A.P.Separation and concentration of biological compounds are current subjects of interest due to their high cost in comparison to the total manufacture cost. For that purpose electrolytes may be employed as they can effect significantly biochemicals solubilities, a feature that has been used for salt-induced precipitation of proteins.
- Salting-in with a salting-out agent: explaining the cation specific effects on the aqueous solubility of amino acidsPublication . Tomé, L.I.N.; Pinho, Simão; Jorge, M.; Gomes, J.R.B.; Coutinho, João A.P.Although the understanding of ion specific effects on the aqueous solubilities of biomolecules is crucial for the development of many areas of biochemistry and life sciences, a consensual and well-supported molecular picture of the phenomena has not yet been established. Mostly, the influence of cations and the nature of the molecular interactions responsible for the reversal of the Hofmeister trend in aqueous solutions of amino acids and proteins are still defectively understood. Aiming at contributing to the understanding of the molecular-level mechanisms governing the cation specific effects on the aqueous solubilities of biocompounds, experimental solubility measurements and classical molecular dynamics simulations were performed for aqueous solutions of three amino acids (alanine, valine, and isoleucine), in the presence of a series of inorganic salts. The evidence gathered suggests that the mechanism by which salting-in inducing cations operate in aqueous solutions of amino acids is different from that of anions, and allows for a novel and consistent molecular description of the effect of the cation on the solubility based on specific interactions of the cations with the negatively charged moieties of the biomolecules.
- The cation effect on the aqueous solubility of amino acids in the presence of salts : experimental and molecular dynamics simulation studiesPublication . Tomé, L.I.N.; Pinho, Simão; Jorge, M.; Gomes, J.R.B.; Coutinho, João A.P.Aiming at contributing to the understanding the molecular level mechanisms governing the behavior of biocompounds in aqueous saline environments, experimental solubility measurements and molecular dynamics (MD) simulations were performed for aqueous solutions of three amino acids (alanine, valine and isoleucine) in the presence of a series of inorganic salts.
- The cation effect on the aqueous solubility of amino acids in the presence of salts : experimental and molecular dynamics simulation studiesPublication . Tomé, L.I.N.; Pinho, Simão; Jorge, M.; Gomes, J.R.B.; Coutinho, João A.P.The study of the solubility behavior of amino acids and proteins in aqueous electrolyte solutions is of utmost importance to understand the biochemistry of natural systems, develop medical and pharmaceutical responses to diseases induced by biochemical disorders and improve the efficiency of biotechnological processes. Although the effect of common salts on the aqueous solubility of amino acids and proteins is phenomenologically well described [1], the underlying molecular-level mechanisms are far from being elucidated and consensual, in spite of the several explanations proposed during the past century.