Percorrer por autor "Finimundy, Tiane C."
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- Analysis of volatiles of rose pepper fruits by GC/MS: drying kinetics, essential oil yield, and external color analysisPublication . Oliveira, Kênia Borges de; Carocho, Marcio; Finimundy, Tiane C.; Resende, Osvaldo; Célia, Juliana Aparecida; Gomes, Francileni Pompeu; Quequeto, Wellytton Darci; Bastos, Fabiano José de Campos; Barros, Lillian; Ferreira Junior, Weder NunesCondiments and culinary supplements are subjected to long-term storage and may undergo physical, chemical, and biological changes that can influence their quality. )us, the objective of the present study was to analyze the drying kinetics of rose pepper (Schinus terebinthifolius Raddi) fruits in an oven with forced air circulation at different temperatures, namely, 45, 55, 65, and 75°C, and determine the effective diffusion coefficient and activation energy using different mathematical models. Furthermore, the effects of the different drying temperatures were analyzed for external color parameters and yield of essential oil contents by gas chromatography coupled to a mass spectrometer. Of the ten models used for fitting, )ompson’s model was one with the best fitting to represent the drying of rose pepper fruits. )e diffusion coefficient increases with the elevation of drying air temperature, described by the Arrhenius equation, with activation energy of 53.579 kJ·mol− 1. )e color of the fruits decreased in lightness (L∗) with the increase in temperature. Of the thirty-eight terpenes identified, α-pinene and cis-ocimene were the most abundant, with the overall highest yield being found at a drying temperature of 45°C.
- Ananas comosus L. bio-waste as a source of bioactive compounds with health benefitsPublication . Moreira, Bruna; Caleja, Cristina; Finimundy, Tiane C.; Pinela, José; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Barros, LillianPineapple ( Ananas comosus L.) is a fruit appreciated and consumed worldwide not only because it is recognized for nutritional properties, but also for the beneficial characteristics that help in the development of the organism [1]. Although only the pulp is consumed, several studies have been exploring different parts of the fruit, as they have high amounts of bioactive compounds of interest. Thus, and since the food industry annually produces tons of waste that are not properly used [2], this work aimed at the characterization of the pineapple peel and crown in order to enhance this bio-waste and a circular bioeconomy. Heat-assisted hydroethanolic extraction was used to recover compounds subsequently identified and quantified by High-Performance Liquid Chromatography coupled with a diode array detector and electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS). Twenty phenolic compounds were identified in both peel and crown extracts, among them, phenolic acids and flavonoids. The main detected compounds were caffeic acid derivatives, namely caffeine putrescine and flavones such as apigenin 6,8- C -diglucoside. The antioxidant activity of the extracts was tested and proved through two in vitro tests: the lipid peroxidation inhibition test (TBARS) and the oxidative hemolysis inhibition test (OxHLIA). The antiproliferative activity of both extracts was evaluated in tumor and non-tumor cell lines using the sulforhodamine B method, and the anti-inflammatory activity in lipopolysaccharide-activated RAW 264.7 macrophages by the ability to inhibit NO production. The results showed that both extracts had an excellent performance in the cell-based tests of antioxidant activity, highlighting the lower EC 50 values and consequently greater activity for the bark extract. The same trend was seen in the tests of anti-tumor activity, with none of the extracts showing toxicity up to the maximum concentration tested (GI 50 > 400 μg/mL). This study confirms the potential application of pineapple bio-residues, especially the peel, in the food industry as a source of compounds with bioactive properties, contributing to the valorization of this bio-waste.
- Antimicrobial, cytotoxic and antioxidante activity of Amaranthaceae plants: a renewable source of nutrients and phenolic compounds.Publication . Núñez Estévez, Bernabé; Finimundy, Tiane C.; Carpena Rodríguez, María; Otero, Paz; Barral Martínez, Marta; Pires, Tânia C.S.; Mandim, Filipa; Pinela, José; García-Pérez, Pascual; Simal-Gandara, Jesus; Ferreira, Isabel C.F.R.; Prieto Lage, Miguel A.; Barros, LillianSince the beginning of the 21st century, food products have been subjected to numerous tests to check their quality and presence of harmful agents. Nowadays, consumers awareness about food quality has increased dramatically, paying much attention to the identification of artificial additives, which are normally included as preservative ingredients, among others. This change is also represented in the food industry, where the use of natural food texturizers and preservatives has been replacing some commonly used artificial additives, thus motivating the search for new bioactive compounds from natural sources. Among such bioactive compounds, plant secondary metabolites, in special phenolic compounds, have been largely reported for their health-enhancing properties and preservative effect. In this study, a phytochemical and nutritional characterization of three species from the Amaranthaceae family (Alternanthera sessilis (L.) R., Dicliptera chinensis (L.) Juss. and Dysphania ambrosioides (L.) Mosyakin & Clemants) was performed. The antioxidant and antimicrobial activities and cytotoxicity to tumour and non-tumour cell lines were also evaluated using different methodologies. The results showed a high content of flavonoids in the ethanolic extract of the three species, ranging 11.4 – 15.1 mg/g extract, with apigenin, luteolin and isorhamnetin derivatives being the most abundant compounds. On the other hand, the results from nutritional analysis showed a high protein content in the species studied (13.9- 16.9 ± 0.1 g/100 g of dry weight, dw), together with the presence of organic acids, such as oxalic and succinic acids. Concerning the reported bioactivities of the plant extracts, the results for cytotoxic activity showed GI50 values ranging from 263 ± 12 μg/mL to 188 ± 14 μg/mL towards different tumor cell lines (colon carcinoma, Caco-2; breast adenocarcinoma, MCF-7; and non-small cell lung, NCI-H460) and no cytotoxic activity in the control cell line (Vero). Moreover, with respect to antimicrobial activity, the A. sessilis extract showed minimal inhibitory concentrations (MIC) of 5 mg/mL against Morganella morganii, as well as the D. abrosioides extract against methicillin-resistant Staphylococcus aureus (MRSA). For the antioxidant activity determined by the oxidative haemolysis assay (OxHLIA), the D. abrosioides extract showed an IC50 of 66 ± 10 μg/mL. Overall, these results suggest that ethanolic extracts from the characterized Amaranthaceae species, exhibiting a high bioactive potential, can be considered as promising natural ingredients or additives in the food industry.
- Apoptosis induction by Pleurotus sajor-caju (Fr.) Singer extracts on colorectal cancer cell linesPublication . Finimundy, Tiane C.; Abreu, Rui M.V.; Bonetto, Natalia; Scariot, Fernando J.; Dillon, Aldo J.P.; Echeverrigaray, Sergio; Barros, Lillian; Ferreira, Isabel C.F.R.; Henriques, João A.P.; Roesch-Ely, MarianaPleurotus sajor-caju (PSC) is an edible mushroom used in food supplements, presenting antitumor properties through induction of cell death pathways. The PSC potential against colorectal cancer was analyzed by exposing HCT116wt cells to different PSC extracts. The PSC n-hexane extract (PSC-hex) showed the highest cytotoxicity effect (IC50 value 0.05 mg/mL). The observed cytotoxicity was then associated to apoptosis-promoting and cell cycle-arrest pathways. PSC-hex was able to induce apoptosis related to breakdown of mitochondrial membrane potential and ROS generation. The absence of cytotoxicity in HTC116-p53 and HTC116-Bax cells, alongside with an increase in p53, Bax and Caspase-3 expression, and decrease in Bcl-2 expression, supports that the proapoptotic effect is probably induced through a p53 associated pathway. PSC-hex induced cell cycle arrest at G2/ M in HCT116wt without cytotoxicity in HTC116-p21 cells. These findings suggest that a p21/p53 cell cycle regulation pathway is probably disrupted by compounds present on PSC-hex. Identification of the major components was then performed with ergosta-5,7,22-trien-3β-ol representing 30.6% of total weight. In silico docking studies of ergosta-5,7,22-trien-3β against Bcl-2 were performed and results show a credible interaction with the Bcl-2 hydrophobic cleft. The results show that PSC-hex can be used as supplementary food for adjuvant therapy in colorectal carcinoma.
- Assessment of the use of common juniper (Juniperus communis L.) foliage following the cascade principlePublication . Mediavilla, Irene; Bados, Raquel; Barros, Lillian; Xavier, Virginie; Finimundy, Tiane C.; Pires, Tânia C.S.P.; Heleno, Sandrina A.; Calhelha, Ricardo C.; Amaral, Joana S.; Rizzo, Andrea Maria; Casini, David; Lombardi, Giacomo; Chiaramonti, David; Camara, Miguel; Suarez, Ana; Ardid, Tomas; Esteban Pascual, Luis SaulJuniperus communis L. is a species commonly grown in regions of the Northern Hemisphere, and is a good candidate to be cultivated in marginal lands. Plants coming from a pruning performed in a natural population located in Spain were used to assess the yield and quality of different products obtained following the cascade principle. A total of 1050 kg of foliage biomass were crushed, steam-distilled, and separated into fractions to produce biochar and absorbents for the pet industry using pilot plants. The obtained products were analysed. The essential oil, with a yield of 0.45% dry basis and a qualitative chemical composition similar to that described for the berries in international standards or monographs, showed antioxidant activity with promising CAA results (inhibition of 89% of the cell's oxidation). However, regarding antibacterial and antifungal activities, it only inhibited the growth of microorganisms at the maximum concentration tested, 2.5%. Concerning the hydrolate, it did not show bioactivity. Regarding the biochar, whose yield was 28.79% dry basis, interesting results were obtained for its characterisation as a possible soil improver for agronomic purposes (PFC 3(A)). Finally, promising results were obtained regarding the use of common juniper as absorbent, taking into account the physical characterisation and odour control capacity.
- Assessment of the use of common juniper (Juniperus communis L.) foliage following the cascade principle: essential oil, absorbents and biocharPublication . Mediavilla, Irene; Bados, Raquel; Barros, Lillian; Xavier, Virginie; Finimundy, Tiane C.; Amaral, Joana S.; Rizzo, Andrea Maria; Casini, David; Lombardi, Giacomo; Cámara, Miguel; Suárez, Ana; Ardid, TomásThe main objective of the BeonNAT project is to create added-value bio-based products by growing underused tree and shrub species in marginal land. One of the species selected within the project is juniper (Juniperus communis L). Foliage biomass coming from a natural population in Spain was selected and a first sampling of wild biomass (1000 kg fresh plants with stem diameters below 50 mm) was performed. This biomass was distilled and, after it, different alternatives were defined in the project to obtain bioproducts from the distilled biomass following the cascade concept. Among them, absorbents for the pet industry and biochar have been selected in this work. Concerning the steam distillation, an essential oil yield of 0.44% (w/w d.b) was obtained and the characterisation of the essential oil compounds showed that it was close to meet the limits considered by the ISO 8897:2010. A fraction of the distilled biomass was used to produce pellets, whose quality as absorbents for the pet industry was promising. Finally, the rest of the distilled biomass was used to obtain biochar with a yield of 28.79 % (w/w d.b) and characteristics which indicated a good degree of carbonisation and stability.
- Authenticity and Bioactive Markers Search in the Phenolic‐Rich Extracts of Asteraceae Medicinal Plants Through Integrative Computational ChemometricsPublication . Garcia Perez, Pascual; Garcia‐Oliveira, Paula; Finimundy, Tiane C.; Pinela, José; Calhelha, Ricardo C.; Nenadić, Marija; Soković, Marina; Simal‐Gandara, Jesus; Barros, Lillian; Prieto Lage, Miguel A.The Asteraceae family has been of significant concern for ethnobotanical studies, thanks to its health-promoting properties linked to a plethora of bioactive compounds, among which phenolic compounds play a critical role. In this work, a workflow based on computational chemometrics was employed to assess the authenticity and biomarker search of five key Asteraceae species commonly employed in traditional medicine. The UHPLC-DAD- ESI/ MS–MS phenolic profile of Asteraceae extracts was combined with the evaluation of several in vitro biological properties. Caffeoylquinic acids (CQAs), chicoric acids, and flavonoid glycosides were reported as authenticity markers of Achillea millefolium, Taraxacum officinale, and Arnica montana, respectively. The integration of phenolic profile and in vitro bioactivities provide insights for the identification of trans 3,5-O- dicaffeoylquinic acid (3,5-O- diCQA) and isorhamnetin glycosides as the major antioxidant agents in Asteraceae extracts, whereas several CQAs and caffeoyl-deoxy- octulopyranosonic acids have been reported as responsible for their cytotoxic and anti-inflammatory activities. These results shed light on the authentication and quality evaluation of Asteraceae extracts, along with the characterization of their functional properties, leading to their application in the design of novel plant-based functional foods.
- Avaliação de subprodutos de soja (Glycine max), uma potencial fonte de nutrientes e compostos bioativosPublication . Sprea, Rafael M.; Finimundy, Tiane C.; Pinela, José; Calhelha, Ricardo C.; Pires, Tânia C.S.; Amaral, Joana S.; Prieto Lage, Miguel A.; Barros, LillianA soja (Glycine max) tem desempenhado um papel relevante na alimentação humana do século 21, não só através de produtos à base do grão, como pelo seu óleo, mas também pela sua utilização na produção de bebida vegetal [1]. Esta última tem-se tornado cada vez mais popular, aumentando a quantidade de grão de soja utilizado e, consequentemente, a quantidade de resíduos gerados. Por forma a contribuir para a implementação de uma economia circular, este trabalho teve como objetivo a caracterização química e nutricional de subprodutos provenientes da indústria de produção de bebidas vegetais de soja, nomeadamente okara e casca de soja. A composição proximal dos resíduos foi determinada pelos métodos oficiais da AOAC, os açúcares livres, tocoferóis e ácidos orgânicos por cromatografia líquida com detetor de índice de refração (HPLC-RI), de fluorescência (HPLC-FL) e de díodos (UPLC-DAD), respetivamente, o perfil de ácidos gordos por cromatografia gasosa com detetor de ionização de chama (GC-FID), e os compostos fenólicos por HPLC acoplado a um DAD em série com espectrometria de massa (HPLC-DAD-ESIMS/ MS). Adicionalmente, procedeu-se à avaliação da atividade antimicrobiana face a oito estirpes bacterianas multirresistentes causadoras de doenças alimentares e duas estirpes de fungos contaminantes de alimentos, e da atividade antioxidante pelos ensaios de inibição da peroxidação lipídica (TBARS), inibição de hemólise oxidativa (OxHLIA) e atividade celular antioxidante (CAA). Relativamente ao okara, este subproduto apresentou elevada humidade, sendo o macronutriente predominante as proteínas. Na casca, verificou-se uma predominância de carboidratos e fibras, e baixo teor de humidade. Na fração lipídica, ambos os subprodutos apresentaram uma predominância de ácidos gordos polinsaturados, sendo os maioritários os ácidos oleico e linoleico para o okara e para a casca, respetivamente. Foram detetadas 3 isoformas de Vitamina E, sendo o γ- e δ-tocoferol identificados no Okara, e o α- e δ-tocoferol na casca do grão de soja, verificando-se uma predominância de δ-tocoferol em ambos os subprodutos. O ácido oxálico foi o único ácido orgânico identificado no okara, enquanto o ácido cítrico foi o predominante na casca de soja. Os elagitaninos e isoflavonas foram os compostos fenólicos maioritários na casca dos grãos de soja, sendo as isoflavonas igualmente predominantes no okara. Ambos os extratos apresentaram propriedades antioxidantes satisfatórias, e no geral apresentaram uma inibição superior contra o crescimento de fungos comparativamente a bactérias. Os resultados obtidos demonstram que os subprodutos de soja têm uma composição interessante do ponto de vista nutricional, podendo ser uma fonte de compostos bioativos e um recurso para o desenvolvimento de novos ingredientes funcionais para a indústria alimentar.
- Bioactive and Phenolic Profiles in Pinus pinaster Bark: A Comparative Study of Microwave and Ultrasound Extraction MethodsPublication . Barros, Diana; Alonso-Esteban, José Ignacio; Finimundy, Tiane C.; Pereira, Carla; Vaz, Josiana A.; Pereira-Pinto, Ricardo; Fernandes, Élia; Pires, Preciosa; Santos, Joana; Barros, Lillian; Vaz-Velho, ManuelaThis study conducted a comprehensive comparison of two green extraction methods, microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE), for recovering bioactive phenolic compounds from Pinus pinaster bark. The goal was to valorize timber industry waste and enhance the value of by-products through the development of eco-friendly processes to extract phenolic compounds from Pinus pinaster Aiton subsp. atlantica in northwest Portugal. MAE achieved significantly higher extraction yields than UAE (11.13 vs. 3.47 g extract/100 g bark) and superior total phenolic content (833 vs. 514 mg GAE/g). MAE extracts also exhibited enhanced antioxidant activity in most assays tested (DPPH, ABTS, ORAC, and OxHLIA), while both extracts effectively inhibited lipid peroxidation (TBARS) and showed activity against Gram-positive bacteria. Phenolic profile analysis revealed that MAE recovered a substantially higher amount of total phenolic compounds (230.0 mg/g) compared to UAE (86.95 mg/g), with procyanidins identified as the predominant compounds. The greater recovery of this complex procyanidin mixture by MAE is strongly associated with the enhanced bioactivities observed. Overall, this study confirms MAE as a highly efficient and sustainable technology for transforming pine bark waste into valuable antioxidant and antimicrobial extracts with potential applications in the food and pharmaceutical industries.
- Bioactive properties of Sanguisorba minor L. cultivated in central Greece under different fertilization regimesPublication . Finimundy, Tiane C.; Karkanis, Anestis; Fernandes, Ângela; Petropoulos, Spyridon Α.; Calhelha, Ricardo C.; Petrović, Jovana; Soković, Marina; Rosa, Eduardo; Barros, Lillian; Ferreira, Isabel C.F.R.In this study, the chemical characterization and bioactive properties of S. minor cultivated under different fertilization rates (control, half rate and full rate) were evaluated. Twenty-two phenolic compounds were identified, including five phenolic acids, seven flavonoids and ten tannins. Hydrolysable tannins were prevalent, namely Sanguiin H-10, especially in leaves without fertilization (control). Roots of full-rate fertilizer (660 Kg/ha) presented the highest flavonoid content, mainly catechin and its isomers, whereas half-rate fertilizer (330 Kg/ha), presented the highest content of total phenolic compounds, due to the higher amount of ellagitannins (lambertianin C: 84 ± 1 mg/g of dry extract). Antimicrobial activities were also promising, especially against Salmonella typhimurium (MBC = 0.44 mg/mL). Moreover, root samples revealed activity against all tested cell lines regardless of fertilization rate, whereas leaves were effective only against HeLa cell line. In conclusion, S. minor could be a source of natural bioactive compounds, while fertilization could increase phenolic compounds content.