Percorrer por autor "Edrada-Ebel, RuAngelie"
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- Metabolomic approach to assess the effect of the foraging year on the anti-elastase activity of Cytinus hypocistis (L.) L.Publication . Silva, Ana Rita; Ayuso, Manuel; García, Pablo A.; Barros, Lillian; Edrada-Ebel, RuAngelieCytinus hypocistis (L.) L. is a wild edible parasitic plant on numerous members of the Cistaceae family. Although its bioactive properties were potentially attributed to its hydrolysable tannin content, its chemical composition is largely unknown, and the bioactive substances have not yet been identified [1,2]. Thus, this work aimed to investigate its potential to inhibit human elastase, an important anti-ageing mechanism. The plant material was collected in Castro Daire, Portugal, from the host species Halimium lasianthum (Lam.) Greuter on the same calendar date in June for three consecutive years (2018, 2019, and 2020). Metabolomic- based analytical techniques were applied to disclose the discriminating features of the anti-elastase activity of nine hydroethanolic extracts (three per year). The discriminating features among samples were identified using a multivariate PLS-DA of the LC-HRMS data. Dereplication of the discriminating metabolites obtained from the loadings plot revealed that year-one had the best inhibitory activity with IC50s of 13 to 22 μg/mL and were comprised of high molecular weight compounds ranging between 500 and 950 Da. However, the second-year collection exhibited a significant decrease in the biological activity of 120 to 128 μg/mL indicating the occurrence of low molecular weight compounds between 150 and 300 Da. Year three gave IC50s of 74 to 109 μg/mL. This study offers an evidential hypothesis on the effect of annual environmental changes on the chemical profile of a parasitic plant. Albeit, the relative abundance of the discriminating features was minimal, it was significant enough to affect the anti-elastase bioactivity of the extracts
- Unveiling the metabolites underlying the skin anti-ageing properties of Cytinus hypocistis (L.) L. through a biochemometric approachPublication . Silva, Ana Rita; Ayuso, Manuel; García, Pablo A.; Barros, Lillian; Edrada-Ebel, RuAngelieThe genus Cytinus, recognised as one of the most enigmatic in the plant kingdom, has garnered attention for its bioactive potential, particularly its skin anti-ageing properties. Despite this recognition, much remains to be accomplished regarding deciphering and isolating its most active compounds. Hypothesis: This study aimed to identify the compounds responsible for C. hypocistis skin anti-ageing potential. Methods: Using multivariate analysis, a biochemometric approach was applied to identify the discriminant metabolites by integrating extracts' chemical profile (Liquid Chromatography–High-Resolution Mass Spectrometry, LC[sbnd]HRMS) and bioactive properties. The identified bioactive metabolite was structurally elucidated by 1D and 2D Nuclear Magnetic Resonance (NMR). Results: Among the studied bioactivities, the anti-elastase results exhibited a significant variation among the samples from different years. After the biochemometric analysis, the compound 2,3:4,6-bis(hexahydroxydiphenoyl)glucose, with a molecular mass of 784.075 Da, was structurally elucidated as the discriminant feature responsible for the outstanding human neutrophil elastase inhibition. Remarkably, the subfraction containing this compound exhibited a tenfold improvement in neutrophil elastase inhibition efficacy compared to the crude extract; its effectiveness fell within the same range as SPCK, a potent irreversible neutrophil elastase inhibitor. Moreover, this subfraction displayed no cytotoxicity or phototoxicity and excellent efficacy for the tested anti-ageing properties. Conclusions: Hydrolysable tannins were confirmed as the metabolites behind C. hypocistis skin anti-ageing properties, effectively mitigating critical molecular mechanisms that influence the phenotypically distinct ageing clinical manifestations. Pedunculagin was particularly effective in inhibiting neutrophil elastase, considered one of the most destructive enzymes in skin ageing.