Percorrer por autor "Danneels, Ellen"
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- An unprecedented large-scale survey of honey bee mitochondrial diversity in Europe: c-lineage dominance and the need for conservation effortsPublication . Li, Fernanda; Costa, Maíra; Lopes, Ana Rita; Gonçalves, Telma; Henriques, Dora; Quaresma, Andreia; Yadró Garcia, Carlos A.; Albo, Alexandre; Blažytė-Čereškienė, Laima; Brodschneider, Robert; Brusbardis, Valters; Carreck, Norman L.; Charistos, Leonidas; Chlebo, Robert; Coffey, Mary F.; Dahle, Bjørn; Danneels, Ellen; Dobrescu, Constantin; Dupleix-Marchal, Anna; Filipi, Janja; Gajda, Anna; Gratzer, Kristina; Groeneveld, Linn Fenna; Hatjina, Fani; Johannesen, Jes; Kolasa, Michal; Körmendy-Rácz, János; Kovačić, Marin; Kristiansen, Preben; Martikkala, Maritta; McCormack, Grace P.; Martín-Hernández, Raquel; Pavlov, Borce; Pietropaoli, Marco; Poirot, Benjamin; Radev, Zheko; Raudmets, Aivar; René-Douarre, Vincent; Roessink, Ivo; Škerl, Maja Ivana Smodiš; Soland-Reckeweg, Gabriele; Titera, Dalibor; Van der Steen, Jozef; Varnava, Andri; Vejsnæs, Flemming; Webster, Matthew T.; Fedoriak, Mariia M.; Zarochentseva, Oksana; Graaf, Dirk C. de; Pinto, M. AliceEurope is home to ten Apis mellifera subspecies, which belong to three mitochondrial lineages: the Western European (M), Eastern European (C), and African (A). However, the long-standing human-mediated movement of queens, primarily of C-lineage ancestry, has threatened the genetic integrity of many of these native subspecies through introgression and replacement. This has led to the establishment of conservation programs to recover the native lines in some European countries. The maternally-inherited mitochondrial DNA (mtDNA), particularly the highly polymorphic intergenic region tRNAleu-cox2, has been the marker of choice for assessing honey bee variation and introgression at large geographical scales. Herein, we will show the results of the tRNAleu-cox2 variation obtained from over 1200 colonies sampled across the range of the ten subspecies and covering 33 European countries. These revealed that apart from a few countries (Portugal, Spain, and Ireland) and isolated protected populations, European populations are predominantly dominated by C-lineage haplotypes, and many native subspecies exhibit a signature of C-derived introgression. In conclusion, this unprecedented survey of honey bee diversity across Europe underscores the concerning dominance of C-lineage genetic variation, highlighting the urgent need for strategic conservation efforts to preserve the native genetic diversity of Apis mellifera.
- Bio-Monitoring of environmental pollution using the citizen science approachPublication . Van der Steen, Jozef; Amaral, Joana S.; Baveco, Hans; Blanco Muñoz, Patricia; Brodschneider, Robert; Brusbardis, Valters; Buddendorf, Bas; Carreck, Norman L.; Danneels, Ellen; Charistos, Leonidas; Graaf, Dirk C. de; Díaz Galiano, Francisco José; Fernández-Alba, Amadeo R.; Ferrer-Amate, Carmen; Formato, Giovanni; Gómez Ramos, María José; Gratzer, Kristina; Gray, Alison; Hatjina, Fani; Henriques, Dora; Kasiotis, Konstantinos; Kilpinen, Ole; Lopes, Ana; Martínez Bueno, María Jesús; Murcia-Morales, María; Pietropaoli, Marco; Pinto, M. Alice; Quaresma, Andreia; Rufino, José; Roessink, Ivo; Vejsnæs, Flemming; Zafeiraki, EffrosyniHoneybee colonies are excellent bio-samplers of biological material such as nectar, pollen, and plant pathogens, as well as non-biological material such as pesticides or airborne contamination. The INSIGNIA-EU project aims to design and test an innovative, non-invasive, scientifically proven citizen science environmental monitoring protocol for the detection of pesticides, microplastics, heavy metals, and air pollutants by honey bee colonies http://insignia-eu.eu. In the pilot INSIGNIA project (2018-2021), a protocol was developed and tested for citizen-science-based monitoring of pesticides using honeybees. As part of the project, biweekly pollen was obtained from sentinel apiaries over a range of European countries and landscapes and analysed for botanical origin, using state-of-theart molecular techniques such as metabarcoding. An innovative non-biological matrix, the “APIStrip”, was also proved to be very efficient for detecting the residues of 273 agricultural pesticides and veterinary products, both authorized and unauthorized. The data collected are used to develop and test a spatial modelling system aimed at predicting the spatiallyexplicit environmental fate of pesticides and honeybee landscape-scale pollen foraging, with a common underlying geo-database containing European land-use and land-cover data (CORINE), the LUCAS database (landcover) supplemented with national data sets on agricultural and (semi-) natural habitats. After a call by the European Commission, a new 2 years project was granted aiming to present a comprehensive pan-European environmental pollution monitoring study with honey bees. Although pesticides used in agriculture, are a known hazard due to their biological activity, other pollutants, have even been recognized as such, for which we have not been aware of their impact for many years. An example is air pollution which increased while our societies industrialized and is currently regarded as the single largest environmental health risk in Europe (https://www.eea.europa.eu/). Unfortunately, other pollutants such as heavy metals, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, airborne particulate matter, and microplastics have also reached our environment. The outcome of this project will provide the first standardized EU-wide monitoring of all types of environmental pollutants with honey bee colonies. The project is funded by the EU, under the N° 09.200200/2021/864096/SER/ ENV.D.2 contract.
- Large Scale Mitochondrial Evidence of C-lineage Dominance in European Honey BeesPublication . Li, Fernanda; Lopes, Ana Rita; Costa, Maíra; Henriques, Dora; Quaresma, Andreia; Yadró Garcia, Carlos A.; Albo, Alexandre; Čereškienė, Laima Blažytė; Brodschneider, Robert; Brusbardis, Valters; Carreck, Norman L.; Charistos, Leonidas; Chlebo, Robert; Coffey, Mary F.; Dahle, Bjørn; Danneels, Ellen; Dobrescu, Constantin; Dupleix-Marchal, Anna; Filipi, Janja; Gajda, Anna; Gratzer, Kristina; Groeneveld, Linn Fenna; Hatjina, Fani; Johannesen, Jes; Kolasa, Michal; Körmendy-Rácz, János; Kovačić, Marin; Kristiansen, Preben; Martikkala, Maritta; McCormack, Grace P.; Martín-Hernández, Raquel; Pavlov, Borce; Poirot, Benjamin; Pietropaoli, Marco; Radev, Zheko; Raudmets, Aivar; René-Douarre, Vincent; Roessink, Ivo; Škerl, Maja Ivana Smodiš; Soland, Gabriele; Titera, Dalibor; Van der Steen, Jozef; Varnava, Andri; Vejsnæs, Flemming; Fedoriak, Mariia M.; Zarochentseva, Oksana; Webster, Matthew T.; Graaf, Dirk C. de; Pinto, M. AliceIn Europe, distribution of the several endemic honey bee (Apis mellifera) subspecies has suffered a considerable shift in the last century. In particular, beekeepers tend to favour subspecies of Eastern European ancestry (C-lineage), such as the Italian honey bee (A. m. ligustica), due to their perceived docility and high honey production. As a result, large scale migratory beekeeping and trade of C-lineage queens have exposed the native European honey bees to introgression and replacement, jeopardizing their genetic integrity and locally adapted traits. The maternally-inherited and highly polymorphic mitochondrial intergenic region tRNAleu-cox2 is routinely used for the assessment of honey bee diversity and introgression at large geographical scales. In this study, we conducted a survey on tRNAleu-cox2 variation in more than 1300 colonies from 33 European countries to assess current status of mitochondrial diversity patterns in Europe. Total genomic DNA was extracted from the bee thorax followed by PCR amplification of the tRNAleu-cox2 region and sequencing. The haplotypes were identified using alignments in MEGA 11. A clear dominance of C-lineage haplotypes was found (65%). The most prevalent C-lineage haplotype was C2 (54%) followed by C3 (23%) and C1(22%). Contrary to this trend, in Portugal, Spain and Ireland, less than 10% of the colonies exhibit the C-haplotype. Furthermore, the analysed apiaries in isolated protected areas (n=7) revealed a high proportion of colonies of western European (M-lineage) ancestry (80%) with M4 as the most frequent haplotype. African haplotypes (A lineage) were also found, albeit at lower frequencies, and were mainly concentrated in the Iberian Peninsula (9%). Intensive queen breeding and migratory beekeeping is homogenizing the gene pool of European bee populations. This survey of honey bee maternal diversity across Europe highlights the alarming dominance of C-lineage haplotypes and underscores the importance of conservation apiaries, as they have effectively preserved the autochthonous M-lineage subspecies in different countries. This work was conducted in the framework of the project Better-B, funded by the European Union, the Swiss State Secretariat for Education, Research, and Innovation, and UK Research and Innovation under the UK government's Horizon Europe funding guarantee (grant number 10068544).
- Maternal origin of honeybee (Apis mellifera) colonies from across EuropePublication . Lopes, Ana; Costa, Maíra; Tounakti, Sahar; Henriques, Dora; Quaresma, Andreia; Yadró Garcia, Carlos A.; Albo, Alexandre; Blažytė-Čereškienė, Laima; Broodschneider, Robert; Brusbardis, Valters; Carreck, Norman L.; Charistos, Leonidas; Chlebo, Robert; Coffey, Mary F.; Danneels, Ellen; Dobrescu, Constantin; Filipi, Janja; Gajda, Anna; Galea, Thomas; Gratzer, Kristina; Hatjina, Fani; Johannesen, Jes; Körmendy-Rácz, János; Kovačić, Marin; Preben, Kristiansen; Martikkala, Maritta; Martín-Hernández, Raquel; Pietropaoli, Marco; Poirot, Benjamin; Radev, Zheko; Raudmets, Aivar; Douarre, Vincent; Rodriguez-Flores, Maria Shantal; Roessink, Ivo; Ivana, Maja; Škerl, Maja Ivana Smodiš; Titera, Dalibor; Van der Steen, Jozef; Varnava, Andri; Vejsnæs, Flemming; Webster, Matthew T.; Graaf, Dirk C. de; Pinto, M. AliceWorldwide commercial beekeeping poses a threat to the native origin of the honeybee (Apis mellifera), with beekeepers favouring subspecies of Eastern European C-lineage ancestry, due to their docile behaviour and high honey production traits. In many parts of western and northern Europe, queens of Western European M-lineage ancestry have been massively replaced by queens of C-lineage ancestry, and this has led to the development of conservation programs aiming at recovering native lines. The maternally-inherited mitochondrial DNA (mtDNA), particularly the intergenic region tRNAleu-cox2, has been the marker of choice for assessing honey bee variation at large geographical scales. Herein, we will show the results of the mtDNA analysis of over 850 colonies collected across 28 European countries. These samples were subjected to DNA extraction, followed by PCR, and Sanger sequencing. The analysis of the sequences was conducted in Mega 11. The results indicated that, apart from Portugal, Spain, and the conservation centres in France and Denmark, where the colonies exhibited African or M haplotypes, the remaining countries are dominated by colonies of C-lineage maternal ancestry. In conclusion, this unprecedented mtDNA analysis conducted across Europe underscores the worrying dominance of C-lineage genetic variation, highlighting the urgent need for strategic conservation efforts to preserve the native genetic diversity of Apis mellifera.
- Synthetic varroacides in honey bee colonies: A comprehensive monitoring program across the European UnionPublication . Steen, Jozef J.M. Van der; Brodschneider, Robert; Brusbardis, Valters; Buddendor, Bas; Carreck, Norman; Danneels, Ellen; Graaf, Dirk C. de; Gratzer, Kristina; Gray, Alison; Hatjina, Fani; Kasiotis, Konstantinos M.; Kilpinen, Ole; Martínez, José Antonio; Murcia-Morales, María; Martinez-Bueno, Maria Jesus; Oller-Serrano, José Luis; Pietropaoli, Marco; Pinto, M. Alice; Quaresma, Andreia; Roessink, Ivo; Tzanetou, Evangelia; Vejsnæs, Flemming; Fernández-Alba, Amadeo R.Managing Varroa destructor in honey bee colonies remains a constant challenge for beekeepers, requiring a balance between maintaining mite levels low whilst minimizing the negative impacts of miticide treatments on bee health. Synthetic varroacides such as coumaphos, tau-fluvalinate, and amitraz are widely used due to their convenience, but they can have negative impacts on the colony and persist in hive materials, with residues detectable long after application. To investigate the presence and dynamics of these synthetic varroacides, the INSIGNIA-EU initiative conducted a large-scale monitoring program, covering 312 bee hive sites across the European Union. The study employed the APIStrip—a novel, non-invasive passive sampler based on TENAX® sorbent—which, when placed inside the hive, passively adsorbs chemical residues from the internal hive environment. This approach has demonstrated its effectiveness eliminating the need to sample bees, wax, honey, or pollen, while still providing representative contamination data from a single, standardized analytical matrix. This study reports results from APIStrip analyses deployed across all EU countries for residues of amitraz, tau-fluvalinate, and coumaphos, using a harmonized and validated analytical protocol. Additionally, thymol, regarded as an environmentally friendly alternative, was also included in the evaluation as a reference. Sampling was carried out over nine consecutive two-week periods from May to August 2023, ensuring synchronized data collection and enabling direct comparability of results across sites and time points. The study found these miticides to be pervasive across most EU regions, appearing in more than 85% of samples and greatly outnumbering detections of the natural alternative, thymol. In most cases, notable miticide residue concentrations persisted throughout the entire sampling period.
- An unparalleled survey of honey bee genetic diversity in Europe – insights from wing shape, nuclear whole genome, and mitochondrial DNA dataPublication . Pinto, M. Alice; Li, Fernanda; Lopes, Ana Rita; Taliadoros, Demetris; Costa, Maíra; Yadró Garcia, Carlos A.; Cunha, Larissa; Henriques, Dora; Martin Hernandez, Giselle; Albo, Alexandre; Blažytė-Čereškienė, Laima; Brodschneider, Robert; Brusbardis, Valters; Carreck, Norman; Charistos, Leonidas; Chlebo, Robert; Cillia, Giovanni; Coffey, Mary F.; Dahle, Bjorn; Danneels, Ellen; Dobrescu, Constantin; Filipi, Janja; Gajda, Anna; Gratzer, Kristina; Groeneveld, Linn; Hatjina, Fani; Johannesen, Jes; Kolasa, Michal; Körmendy-Rácz, János; Kovačić, Marin; Kristiansen, Preben; Dupleix-Marchal, Anna; Martikkala, Maritta; McCormack, Grace; Martín-Hernández, Raquel; Nanetti, Antonio; Pavlov, Borce; Pietropaoli, Marco; Poirot, Benjamin; Radev, Zheko; Raudmets, Aivar; René-Douarre, Vincent; Roessink, Ivo; Škerl, Maja Ivana Smodiš; Soland, Gabriele; Titera, Dalibor; Van der Steen, Jozef; Varnava, Andri; Vejsnæs, Flemming; Graaf, Dirk C. de; Webster, MathewEurope is home to 10 subspecies of Apis mellifera, classified into four main lineages sensu Ruttner: M (western and northern European), C (southeastern European), A (African), and O (Middle Eastern). However, large-scale movements of honey bee genetic material—driven by transhumance and, more importantly, queen trading—has intensified gene flow, particularly from the C-lineage subspecies A. m. carnica and A. m. ligustica, threatening the genetic integrity of several subspecies and blurring the genetic boundaries between subspecies. Here, we analyzed over 1,200 recently collected samples from apiaries in 33 countries using a multi-marker approach: maternally inherited mitochondrial DNA (tRNAleu-cox2 intergenic region), nuclear genome-wide single nucleotide polymorphisms (SNPs), and wing shape (DeepWings©) to provide the most updated and extensive account of honey bee genetic variation in Europe. Our results reveal a strong dominance of C-lineage ancestry across Europe, with noteworthy introgression signals in island subspecies and A. m. mellifera populations outside conservation apiaries. Notably, all three markers consistently captured these broad introgression patterns. This unprecedented survey highlights a worrying trend of genetic homogenization in European honey bee populations. Moreover, the widespread prevalence of C-lineage genetic introgression underscores the need for conservation efforts to preserve the diversity shaped by evolution, which is crucial for adaptation to growing environmental challenges.
- Wing geometric morphometrics of european honey bee populationsPublication . Costa, Maíra; Gonçalves, Telma; Lopes, Ana; Vitrio, Nathalia; Albo, Alexandre; Blažytė-Čereškienė, Laima; Brodschneider, Robert; Brusbardis, Valters; Carreck, Norman L.; Charistos, Leonidas; Chlebo, Robert; Coffey, Mary F.; Danneels, Ellen; Graaf, Dirk C. de; Dobrescu, Constantin; Filipi, Janja; Gajda, Anna; Gratzer, Kristina; Hatjina, Fani; Johannesen, Jes; Körmendy-Rácz, János; Kovačić, Marin; Kristiansen, Preben; Martikkala, Maritta; Martín-Hernández, Raquel; Pietropaoli, Marco; Poirot, Benjamin; Radev, Zheko; Raudmets, Aivar; Douarre, Vincent; Roessink, Ivo; Škerl, Maja Ivana Smodiš; Titera, Dalibor; Van der Steen, Jozef; Varnava, Andri; Vejsnæs, Flemming; Webster, Matthew T.; Rodrigues, Pedro João; Henriques, Dora; Pinto, M. AliceWing venation patterns have long been used to identify honey bee subspecies, singly or in combination with other morphological traits, using different approaches. Beekeepers have traditionally used identification methods that only require estimations of the Cubital Index, Hantel Index, and/or Discoidal Shift Angle. However, these measures do not consider all the information carried by wing patterns, and a more accurate approach is geometric morphometrics, which requires the annotation of 19 landmarks in the forewing vein junctions. While manual wing annotation is a time-consuming and error-prone endeavour, the recently developed software DeepWings© performs this task automatically, allowing a fast and relatively accurate identification of European honey bee subspecies, and is appropriate for large-scale projects. In this study, we analyse the patterns of forewings extracted from over 850 colonies sampled across 29 European countries. To that end, the right forewings of five workers per colony are photographed with a digital camera attached to a stereomicroscope. The images are then analysed using DeepWings©. Our main objective is to assess (i) how closely the colonies identified by DeepWings© match the endemic evolutionary lineages and subspecies; and (ii) the association between the identification produced by DeepWings© and that inferred from mitochondrial DNA. While geometric morphometrics does not replace molecular tools for the identification of colonies, it can provide a preliminary estimation of their genetic integrity.