Browsing by Author "Corrêa, Vanesa G."
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- Bioaccessibility Performance of Phenolic Compounds from Red Fruits During Simulated Gastrointestinal Digestion and Colonic FermentationPublication . Bortolini, Débora Gonçalves; Barros, Lillian; Maciel, Giselle Maria; Peralta, Rosane M.; Corrêa, Vanesa G.; Finimundy, Tiane Cristine; Haminiuk, Charles Windson IsidoroRed fruits are rich in phenolic compounds, particularly anthocyanins, which contribute to their vibrant colors and health benefits. However, anthocyanins are chemically sensitive during digestion, which affects their bioaccessibility. This study evaluated the impact of simulated gastrointestinal digestion on whole red fruits (oral, gastric, intestinal, and colonic phases). Total phenolic content (TPC) ranged from 8.60−50.41 mg GAE/g DW, total flavonoid content (TFC) from 1.66−10.67 mg CAT/g DW, and total monomeric anthocyanins (TMA) from 0.54−1.28 mg CYA/g DW. Antioxidant activity strongly correlated with TPC (r ≥ 0.91). High-phenolic fruits showed lower bioaccessibility compared with low-phenolic ones. HPLC-DAD-ESI/MSn identified glycosylated anthocyanins, which were highly sensitive; jaboticaba anthocyanins were nonbioaccessible, while 51.83% remained bioaccessible in raspberries. Colonic fermentation further reduced TPC and TFC, and anthocyanins were entirely degraded. These results highlight the need for innovative delivery systems to improve anthocyanin bioaccessibility and functional benefits.
- Effects of in vitro gastrointestinal digestion and colonic fermentation on a rosemary (Rosmarinus officinalis L) extract rich in rosmarinic acidPublication . Gonçalves, Geferson A.; Corrêa, Rúbia C.G.; Barros, Lillian; Dias, Maria Inês; Calhelha, Ricardo C.; Corrêa, Vanesa G.; Bracht, Adelar; Peralta, Rosane M.; Ferreira, Isabel C.F.R.The potential phytochemical losses occurring throughout the sequential steps of in-vitro gastrointestinal digestion and colonic fermentation of a rosemary aqueous extract were investigated. Crude (CE), digested (DE) and fermented (FE) extracts were characterized in terms of their phenolic profile and biological activities. Rosmarinic acid was the phytochemical that underwent the most significate transformation during digestion and fermentation, which amounted to 60% compared to the 26% degradation of the total phenolics. Overall, the simulated digestion step decreased the antioxidant activity estimated by DPPH, ABTS, FRAP, ORAC and TBARS assays. Both CE and DE did not present antiproliferative potential, however, FE exhibited a pronounced cytotoxic activity (GI50 = 116 µg/mL) against HeLa cells. CE and DE showed to be moderate inhibitors of methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus (MSSA), S. aureus, Listeria monocytogenes, whilst the FE acted as a moderate inhibitor of MRSA and MSSA.
- MycosterolsPublication . Uber, Thaís Marques; Backes, Emanueli; Corrêa, Rúbia C.G.; Corrêa, Vanesa G.; Bracht, Adelar; Peralta, Rosane M.Sterols are amphipathic lipids that play essential roles in the physiology of eukaryotic organisms in general. The fungal sterols are collectively known as mycosterols and they exert numerous physiological functions. For humans, the interest on this class of compounds relies heavily on the fact that they can promote health benefits. For this reason, fungal extracts rich in sterols of various forms are valuable and promising ingredients. One of the best-known benefits of mycosterols is their inhibitory actions on cholesterol absorption and biosynthesis, but there are several interesting regulatory and modulatory phenomena that mycosterols can affect and that might eventually be of therapeutic interest. Within this domain, the practical application of mycosterols or mycosterol-enriched fungal extracts presents several challenges. The latter include isolation of novel bioactive mycosterols from still underexploited fungi species, the optimization of existing methodologies for production and recovery, extensive study of their applications and, finally, substantial clinical trials for attesting their health benefits and safety.