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  • Effect of Cryphonectria hypovirus (CHV-1) infection on oxaloacetate acetylhydrolase (OAH) in Cryphonectria parasitica hypovirulent strains
    Publication . Belkhiria, Emna; Jorge, Lurdes; Gouveia, Maria Eugénia; Ben Rejeb, Ines
    Research on chestnut blight, caused by the fungus Cryphonectria parasitica, aims to understand and mitigate its virulence on Castanea sativa Mill, the main host. The use of Cryphonectria hypovirus 1 (CHV-1), known for inducing hypovirulence in the fungus, is one of the main resources of the disease biological control. Conversion of the wild C. parasitica by hypovirulent strains containing the virus leads to reduced fungal pathogenicity. Oxaloacetate acetylhydrolase is one of the enzymes involved in the mechanism of pathogenicity of C. parasitica, and its expression is known to be influenced by CHV- 1 infection. In this study, virulent isolates of C. parasitica were intentionally converted to hypovirulent strains. RNA extraction was performed using two separate methods, with the purpose that the cDNA obtained was used to assess Oxaloacetate acetylhydrolase expression in isogenic converted isolates. In the following step, we screened specific Oxaloacetate acetylhydrolase primers and two other primers to select reference genes for the execution of RT-qPCR experiments. Unfortunately, time constraints hindered the achievement of the expected final results. In addition, a growth analysis of the isolates was performed to decipher significant differences between virulent and isogenic converted isolates.
    2024Dissertação de mestrado Acesso aberto Ver mais