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Title: Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey
Authors: Estevinho, Leticia M.
Pereira, Ana Paula
Moreira, Leandro
Dias, L.G.
Pereira, Ermelinda
Keywords: Honey
Phenolic compounds
Antioxidant activity
Antimicrobial activity
Issue Date: 2008
Publisher: Elsevier
Citation: Estevinho, L.; Pereira, A. P.; Moreira, L.; Dias, L. G.; Pereira, E. (2008) - Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food and Chemical Toxicology. ISSN 0278-6915. 46:12, p. 3774-3779
Abstract: Phenolic compounds of dark and clear honeys from Trás-os-Montes of Portugal were extracted with Amberlite XAD-2 and evaluated for their antioxidant and antimicrobial activities. The antioxidant effect was studied using the in vitro test capacity of scavenge the 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical and of reducing power of iron (III)/ferricyanide complex. The antimicrobial activity was screened using three Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, Staphylococcus lentus) and three Gram-negative bacteria (Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli). The results obtained from the partial identification of honey phenolic compounds by high-performance liquid chromatography with a diode array detector showed that p-hydroxibenzoic acid, cinnamic acid, naringe- nin, pinocembrin and chrysin are the phenolic compounds present in most of the samples analyzed. Anti- oxidant potential was dependent of honey extract concentration and the results showed that dark honey phenolic compounds had higher activity than the obtained from clear honey. In the biological assays, results showed that S. aureus were the most sensitive microrganisms and B. subtilis, S. lentus, K. pneumo- niae and E. coli were each moderately sensitive to the antimicrobial activity of honey extracts. Neverthe- less, no antimicrobial activity was observed in the test with P. aeruginosa.
Peer review: yes
ISSN: 0278-6915
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Appears in Collections:ARN - Artigos em Revistas Indexados ao ISI/Scopus

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