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Abstract(s)
No Instituto Politécnico de Bragança a raça local Churra Galega Bragançana Branca tem sido sujeita, nos últimos 3 anos, a um programa sistemático de aplicação da técnica de inseminação artificial (IA) com sémen fresco ou refrigerado. O presente trabalho foi desenvolvido para testar a eficácia dos seguintes factores – administração prévia de melatonina exógena, tratamento progestagénico curto (FGA) + eCG, dois diluidores seminais (INRA 96® vs. OviXcell®) e dois processos de preservação do sémen (Fresco vs. Refrigerado) – e os seus efeitos sobre a taxa de fertilidade aos 41 dias pós-IA. Foram ainda avaliados alguns parâmetros registados quando da IA – cor e lubrificação da mucosa vaginal, tipo de Os externo, viscosidade do muco cervical, local de deposição do sémen, refluxo cervical e inseminador “A” X “B”.
Na primeira quinzena de Abril, todas as ovelhas Churras Bragançanas Brancas estavam cíclicas. Todas elas responderam ao tratamento FGA + eCG, independentemente de terem sido tratadas ou não com melatonina exógena. Quarenta e um dias após a inseminação artificial 84,1% das ovelhas estavam gestantes.
A taxa de fertilidade foi afectada pelo carneiro dador de sémen, pelo tipo de Os externo, pela viscosidade das secreções cervicais e pelo local de deposição do sémen.
A taxa de fertilidade não foi influenciada pela administração prévia de melatonina, pelo método de preservação do sémen, pelo tipo de diluidor, pela cor e pelo grau de lubrificação da mucosa vaginal, pelo refluxo cervical ou pelo inseminador.
Churra Galega Bragançana Branca ewes have been used in a program of artificial insemination (AI) with fresh or cooled semen developed at the Braganza Polytechnic Institute for the last 3 years. Present study aimed to test the efficiency of different factors – exogenous melatonin pre-treatment, short-term progestogen treatment (FGA) + eCG, two seminal extenders (INRA 96® vs. OviXcell®) and two semen preservation procedures (Fresh vs. Refrigerated) – on ovary activity and fertility rate at forty one days post-AI. During AI several issues were assessed: vagina mucosa colour and lubrication rate, external Os type, cervical mucus viscosity, semen allocation place, cervical reflux incidence and two inseminators’ skills “A” X “B”. All ewes were cycling at the first half of April. The efficiency rate of short-term progestogen treatment + eCG was 100%. Melatonin previous administration had no effect in the ratio of ovulated ewes. About 84.1% of ewes were pregnant at 41 days after AI. Fertility rate was influenced by male semen donor, external Os type, cervical mucus viscosity and semen allocation place. In opposition fertility rate was not affect by exogenous melatonin pre-treatment, semen preservation procedure, semen extender, vagina mucosa color and lubrication, cervical reflux prevalence or inseminator.
Churra Galega Bragançana Branca ewes have been used in a program of artificial insemination (AI) with fresh or cooled semen developed at the Braganza Polytechnic Institute for the last 3 years. Present study aimed to test the efficiency of different factors – exogenous melatonin pre-treatment, short-term progestogen treatment (FGA) + eCG, two seminal extenders (INRA 96® vs. OviXcell®) and two semen preservation procedures (Fresh vs. Refrigerated) – on ovary activity and fertility rate at forty one days post-AI. During AI several issues were assessed: vagina mucosa colour and lubrication rate, external Os type, cervical mucus viscosity, semen allocation place, cervical reflux incidence and two inseminators’ skills “A” X “B”. All ewes were cycling at the first half of April. The efficiency rate of short-term progestogen treatment + eCG was 100%. Melatonin previous administration had no effect in the ratio of ovulated ewes. About 84.1% of ewes were pregnant at 41 days after AI. Fertility rate was influenced by male semen donor, external Os type, cervical mucus viscosity and semen allocation place. In opposition fertility rate was not affect by exogenous melatonin pre-treatment, semen preservation procedure, semen extender, vagina mucosa color and lubrication, cervical reflux prevalence or inseminator.
Description
Mestrado de dupla diplomação com a UTFPR - Universidade Tecnológica Federal do Paraná
Keywords
Ovinos Raça Churra Galega Bragançana Sincronização de cios Inseminação artificial