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Abstract(s)
Tuberaria lignosa (Sweet) Samp. is found in European regions. This plant has antioxidant
properties due to its composition in ascorbic acid and phenolic compounds [1]. Given its
antioxidant properties and its large traditional use, the aim of this work was to study the tumor
cell growth inhibitory potential of aqueous extracts from T. lignosa (prepared by infusion and
decoction).
Cell growth was accessed by the Sulforhodamine B (SRB) assay in three human tumor cell lines:
MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer), and HCT-15 (human
colorectal adenocarcinoma). Both extracts inhibited the growth of the cell lines tested. The most
potent extract was the T. lignosa extract obtained by infusion, in the NCI-H460 cells (presenting
a GI50 of approximately 50 μg/mL).
Further assays were carried out with two different concentrations of this extract in the NCI-H460
cells. The determination of its effect on the cell cycle profile was carried out by analyzing cellular
DNA content by flow cytometry following incubation with propidium iodide. Determination of
cellular apoptosis was performed with the Annexin V-FICT and propidium iodide assay, by flow
cytometry. The expression of apoptotic proteins was carried out by Western Blot. Results showed
that 100 μg/mL or 150 μg/mL of extract caused an increase in the percentage of cells in the G0/G1
phase and a decrease of cells in S phase of the cell cycle. In addition, the extract caused an
increase in the percentage of apoptotic cells, with a decrease in total poly (ADP-ribose)
polymerase (PARP) and pro-caspase 3 levels.
In conclusion, the T. lignosa extract obtained by infusion was more potent in NCI-H460 cells,
altering the cell cycle progression and inducing apoptosis.
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Pedagogical Context
Citation
Pereira, Joana; Rodrigues, Vanessa Lopes; Xavier, Cristina; Lima, Maria João; Lima, Raquel T.; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena (2017). The cell growth inhibitory effect of an aqueous extract of Tuberaria lignosa in NCI-H460 human tumor cells. In IUJUP - 10.º Encontro de Jovens Investigadores da U.PORTO. ISBN 978-989-746-117-0