Publication
Metabolomic approach to assess the effect of the foraging year on the anti-elastase activity of Cytinus hypocistis (L.) L.
| dc.contributor.author | Silva, Ana Rita | |
| dc.contributor.author | Ayuso, Manuel | |
| dc.contributor.author | García, Pablo A. | |
| dc.contributor.author | Barros, Lillian | |
| dc.contributor.author | Edrada-Ebel, RuAngelie | |
| dc.date.accessioned | 2023-03-06T15:26:13Z | |
| dc.date.available | 2023-03-06T15:26:13Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Cytinus hypocistis (L.) L. is a wild edible parasitic plant on numerous members of the Cistaceae family. Although its bioactive properties were potentially attributed to its hydrolysable tannin content, its chemical composition is largely unknown, and the bioactive substances have not yet been identified [1,2]. Thus, this work aimed to investigate its potential to inhibit human elastase, an important anti-ageing mechanism. The plant material was collected in Castro Daire, Portugal, from the host species Halimium lasianthum (Lam.) Greuter on the same calendar date in June for three consecutive years (2018, 2019, and 2020). Metabolomic- based analytical techniques were applied to disclose the discriminating features of the anti-elastase activity of nine hydroethanolic extracts (three per year). The discriminating features among samples were identified using a multivariate PLS-DA of the LC-HRMS data. Dereplication of the discriminating metabolites obtained from the loadings plot revealed that year-one had the best inhibitory activity with IC50s of 13 to 22 μg/mL and were comprised of high molecular weight compounds ranging between 500 and 950 Da. However, the second-year collection exhibited a significant decrease in the biological activity of 120 to 128 μg/mL indicating the occurrence of low molecular weight compounds between 150 and 300 Da. Year three gave IC50s of 74 to 109 μg/mL. This study offers an evidential hypothesis on the effect of annual environmental changes on the chemical profile of a parasitic plant. Albeit, the relative abundance of the discriminating features was minimal, it was significant enough to affect the anti-elastase bioactivity of the extracts | pt_PT |
| dc.description.sponsorship | A. R. Silva is grateful to FCT and FSE for her Doctoral Grant (SFRH/BD/145834/2019). [1] P. Zucca, M. Pintus, G. Manzo, M. Nieddu, D. Steri and A. C. Rinaldi, BMC Res. Notes, 2015, 8, 562. [2] G. Maisetta, G. Batoni, P. Caboni, S. Esin, A. C. Rinaldi and P. Zucca, BMC Complement. Altern. Med., 2019, 19, 82. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Silva, Ana Rita; Ayuso, Manuel; García, Pablo A.; Barros, Lillian; Edrada-Ebel, RuAngelie (2022). Metabolomic approach to assess the effect of the foraging year on the anti-elastase activity of Cytinus hypocistis (L.) L. In Scottish Metabolomics Network Annual Symposium 2022. Aberdeen | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10198/27491 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation | Cytinus hypocistis L. L.: a case study for the development of an innovative, safe, and effective anti-photoaging cosmeceutical formulation | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Cytinus hypocistis (L.) | pt_PT |
| dc.subject | Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology | pt_PT |
| dc.title | Metabolomic approach to assess the effect of the foraging year on the anti-elastase activity of Cytinus hypocistis (L.) L. | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Cytinus hypocistis L. L.: a case study for the development of an innovative, safe, and effective anti-photoaging cosmeceutical formulation | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F145834%2F2019/PT | |
| oaire.citation.conferencePlace | Aberdeen | pt_PT |
| oaire.citation.endPage | 1 | pt_PT |
| oaire.citation.startPage | 1 | pt_PT |
| oaire.citation.title | Scottish Metabolomics Network Annual Symposium 2022 | pt_PT |
| oaire.fundingStream | POR_NORTE | |
| person.familyName | Silva | |
| person.familyName | Ayuso | |
| person.familyName | Barros | |
| person.givenName | Ana Rita | |
| person.givenName | Manuel | |
| person.givenName | Lillian | |
| person.identifier | ABD-5857-2020 | |
| person.identifier | ABC-7764-2020 | |
| person.identifier | 469085 | |
| person.identifier.ciencia-id | 0E1A-35C1-C79A | |
| person.identifier.ciencia-id | 6511-A057-C55F | |
| person.identifier.ciencia-id | 9616-35CB-D001 | |
| person.identifier.orcid | 0000-0001-5819-9065 | |
| person.identifier.orcid | 0000-0002-1697-3857 | |
| person.identifier.orcid | 0000-0002-9050-5189 | |
| person.identifier.rid | J-3600-2013 | |
| person.identifier.scopus-author-id | 35236343600 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
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