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Bioactive and nutritional characterization of stinging nettle (Urtica dioica L.) harvested in Portugal

2019Conference object Open access
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dc.contributor.authorSilva, Jacqueline
dc.contributor.authorDias, Maria Inês
dc.contributor.authorBarros, Lillian
dc.contributor.authorTanamati, Ailey Aparecida Coelho
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorAmaral, Joana S.
dc.date.accessioned2019-06-26T09:11:18Z
dc.date.available2019-06-26T09:11:18Z
dc.date.issued2019
dc.description.abstractStinging nettle (Urtica dioica L.) is an edible wild plant known since ancient times for its dietary and therapeutic benefits. Despite being frequently perceived as being a weed, its use as human food has a long tradition since it was part of the ancient Greek and Roman cuisines and latter it was used as a traditional food by the native American Indians [1]. In Europe, stinging nettle has been consumed as food mainly in periods of famine and scarcity, such as wars. The leaves of this plant can be used in the confection of soups and other dishes such as omelettes, risottos, puree, tarts and consumed as cooked vegetable [2]. Although in the last decades the use of stinging nettle in gastronomy has fallen into disuse, as happen with other wild edible plants, thus it is still traditionally consumed in several regions of the world, such as the Mediterranean region, as part of a cultural and gastronomic heritage. Therefore, this study aimed to perform the nutritional, chemical and bioactive characterization of the leaves of different samples of stinging nettles harvested in Portugal. Fresh plant specimens were collected in the wild in the beginning of March 2017, from two different regions of Portugal, Viseu (40° 39′ 39″ N, 7° 54′ 34″ E) and Vila Real (41° 17′ 45″ N, 7° 44′ 46″). Another sample was collected from the same place in the region of Viseu, three months latter, in June 2017. The samples were evaluated regarding their nutritional composition including moisture, fat, proteins and ash, according to AOAC official procedures, and carbohydrates were determined by difference. Fatty acids were determined by gas chromatography coupled to a flame ionization detector (GC-FID) and phenolic compounds by High Performance Liquid Chromatography coupled to a diode-array and mass spectrometry detector using the electrospray ionization interface (HPLC-DAD-ESI/MSn). The antioxidant activity was evaluated by means of three different in vitro assays: DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power and inhibition of β-carotene bleaching. The antimicrobial susceptibility assay was performed using the Kirby-Bauer disc diffusion method against 4 Gram-positive bacteria (Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis) and 4 Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa). The leaves of stinging nettle samples presented a high percentage of moisture (78.5-83.7 g/100 g fresh leaves) with carbohydrates being the major macronutrient (47.5-50.0 g/100 g, dry basis), while fat was present in very low amounts (3.3-4.0 g/100g, dry basis). A total of 21 fatty acids were identified in the lipid fraction, with α-linolenic acid being the predominant one (41.9-51.3%). The qualitative profile among the 3 samples was identical, although quantitative differences were observed. Regarding phenolic compounds’ composition, a total of 16 compounds, including phenolic acids and flavonoids, were identified and quantified, with only 5 being present simultaneously in the 3 analyzed samples (3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, caffeic acid, isorhamnetin-3-O-rutinoside and quercetin-3-O-rutinoside). Although the sample collected in June in Viseu region was the one with lower content of phenolic compounds, it presented a similar antioxidant activity to the sample from Vila Real, which had the highest content of phenolic compounds. In general, the extracts showed a low activity towards the tested bacteria, with the exception for Pseudomonas aeruginosa, against which all three extracts showed a high activity.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019), and L. Barros and M.I. Dias thank the National funding by FCT, P.I., through the institutional scientific employment program-contract. The authors are grateful to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, Jaqueline; Dias, Maria Inês; Barros, Lillian; Tanamati, Ailey A.; Ferreira, Isabel C.F.R.; Amaral, J.S. (2019). Bioactive and nutritional characterization of stinging nettle (Urtica dioica L.) harvested in Portugal. In XX EuroFoodChem Conference. Porto. ISBN 978-989-8124-26-5pt_PT
dc.identifier.issn978-989-8124-26-5
dc.identifier.urihttp://hdl.handle.net/10198/19358
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectUrtica dioica L.pt_PT
dc.subjectNutritional characterizationpt_PT
dc.subjectPortugalpt_PT
dc.titleBioactive and nutritional characterization of stinging nettle (Urtica dioica L.) harvested in Portugalpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2019/PT
oaire.citation.conferencePlacePorto, Portugalpt_PT
oaire.citation.titleXX EuroFoodChem Conferencept_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDias
person.familyNameBarros
person.familyNameFerreira
person.familyNameAmaral
person.givenNameMaria Inês
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.givenNameJoana S.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0002-3648-7303
person.identifier.ridM-8242-2013
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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