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Colonization patterns of Nosema ceranae in the Azores archipelago

2022Conference object Open access
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dc.contributor.authorLopes, Ana
dc.contributor.authorSegura, Sara K.
dc.contributor.authorMartín-Hernández, Raquel
dc.contributor.authorHenriques, Dora
dc.contributor.authorPinto, M. Alice
dc.date.accessioned2022-10-17T11:35:41Z
dc.date.available2022-10-17T11:35:41Z
dc.date.issued2022
dc.description.abstractNosema ceranae and Nosema apis are pathogens of honey bees that cause nosemosis, a disease implicated in colony losses worldwide. They are obligate intracellular pathogens infecting the midgut epithelial cells of adult honey bees. Although N. ceranae was originally a pathogen specific of Apis cerana, currently, it is found in Apis mellifera throughout most of the world. Due to their confined environments, which limited pathogen transmission and dissemination, islands are unique places for epidemiological studies. There are only a few Varroa destructor-free and possibly even fewer N. ceranae-free honey bee sanctuaries in the world, with the Azores being one. Even though with the exception of Santa Maria and Flores, nosemosis has been present in the Azores since 2008, the causal species has yet to be identified. Hence, this study aimed to determine the prevalence and infection levels of Nosema spp. in the Azorean honey bees. In 2014/2015, 474 colonies were sampled on Faial, Flores, Pico, Graciosa, São Jorge, São Miguel, Santa Maria, and Terceira. Additionally, São Jorge, Santa Maria, Faial, and Terceira were re-sampled in 2020 with a total of 91 colonies. DNA was extracted, and the diagnosis and Nosema spp. loads were obtained by multiplex PCR and RT-qPCR. The findings indicate that N. ceranae appears to be the dominant species in the Azores. N. apis was only detected in 2014/2015 with a very low prevalence (5.1%). N. ceranae prevalence varied between 2.7%, on São Jorge, and 50.7%, on Pico. In 2020, N. ceranae positive colonies increased significantly on Terceira (57.1%) and São Jorge (50.0%). N. ceranae was not detected on Santa Maria in both periods. Average infection levels in positive N. ceranae colonies were usually medium to high (>10-7 ng/μl), with São Jorge colonies displaying the greatest infection intensity (>10-5 ng/ μl). This study highlights the Azores archipelago as a unique place for beekeeping, with islands free of N. ceranae and V. destructor, which are two important stressors that afflict honey bees in the world.pt_PT
dc.description.sponsorshipFinancial support was provided by Portuguese funds through FCT (Fundação para a Ciência e a Tecnologia) in the framework of the project BeeHappy (POCI-01-0145-FEDER-029871). ARL was supported by a PhD scholarship (SFRH/BD/143627/2019) from the FCT. FCT provided financial support by national funds (FCT/MCTES) to CIMO (UIDB/00690/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLopes, Ana Rita; Segura, Sara K.; Martín-Hernández, Raquel; Henriques, Dora; Pinto, M. Alice (2022). Colonization patterns of Nosema ceranae in the Azores archipelago. In ICBEES-International Congress on Bee Sciences. Onlinept_PT
dc.identifier.urihttp://hdl.handle.net/10198/26013
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationPOCI-01-0145-FEDER-029871pt_PT
dc.relationHoneybees Apis mellifera L. in the Azores: developing innovative molecular tools and uncovering host-pathogen interactions in a pre- and post-Varroa destructor invasion
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectNosema apispt_PT
dc.subjectNosema ceranaept_PT
dc.subjectApis melliferapt_PT
dc.subjectReal-time qPCRpt_PT
dc.subjectPrevalencept_PT
dc.subjectInfection levelspt_PT
dc.titleColonization patterns of Nosema ceranae in the Azores archipelagopt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleHoneybees Apis mellifera L. in the Azores: developing innovative molecular tools and uncovering host-pathogen interactions in a pre- and post-Varroa destructor invasion
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F143627%2F2019/PT
oaire.citation.conferencePlaceOnlinept_PT
oaire.citation.endPage61pt_PT
oaire.citation.startPage61pt_PT
oaire.citation.titleICBEES-International Congress on Bee Sciencespt_PT
person.familyNameLopes
person.familyNameHenriques
person.familyNamePinto
person.givenNameAna
person.givenNameDora
person.givenNameM. Alice
person.identifier.ciencia-idF014-6835-054D
person.identifier.ciencia-id291F-986F-07DA
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.orcid0000-0002-1888-2262
person.identifier.orcid0000-0001-7530-682X
person.identifier.orcid0000-0001-9663-8399
person.identifier.scopus-author-id55761737300
person.identifier.scopus-author-id8085507800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublicationd2abd09f-a90c-4cfb-9a60-7fc32f56184d
relation.isAuthorOfPublication0667fe04-7078-483d-9198-56d167b19bc5
relation.isAuthorOfPublication.latestForDiscovery5c8dc714-e63c-4ac6-882e-92deafa4bbe8
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