Advisor(s)
Abstract(s)
The separation of nadolol racemates under high pH reversed-phase preparative chromatography is presented for
the first time.
Three Waters C18 adsorbents (XBridge, Shield and XSelect) are compared for the separation of nadolol racemates
using ethanol:water:diethylamine solvent mixtures. Experimental and simulation results are presented
to compare the separation performances at preparative scale using both the fixed-bed and the simulated moving
bed operations.
The Waters XBridge C18 adsorbent and an ethanol:water:diethylamine solvent mixture are selected as a good
option for the separation of nadolol racemates. The validated methodology allows the separation of a multicomponent
nadolol feed mixture composed by four stereoisomers into two pure racemates (two pairs of enantiomers).
This work introduces the potential of using an initial achiral separation step in the global strategy for
the complete multicomponent separation of the four nadolol stereoisomers.
Description
Keywords
Multicomponent separation Nadolol Reversed-phase preparative chromatography
Pedagogical Context
Citation
Arafah, Rami S.; Ribeiro, António E.; Rodrigues, Alírio E.; Pais, Luís S. (2020). Separation of nadolol racemates by high pH reversed-phase preparative chromatography. Separation and Purification Technology. ISSN 1383-5866. 233, p. 1-13