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How do extraction methodologies influence the biological properties of pomegranate leaves?

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dc.contributor.authorMarcelino, Sandra Andreia
dc.contributor.authorOludemi, Taofiq
dc.contributor.authorMandim, Filipa
dc.contributor.authorFinimundy, Tiane C.
dc.contributor.authorPrieto Lage, Miguel A.
dc.contributor.authorBarros, Lillian
dc.date.accessioned2023-03-21T12:29:37Z
dc.date.available2023-03-21T12:29:37Z
dc.date.issued2022
dc.description.abstractOne of the oldest known plants is the pomegranate tree (Punica granutum L.), native to the Mediterranean region. This plant's edible and non-edible portions have been shown to provide several health advantages. It has long been recognized as a source of bioactive chemicals, such as phenolic acids, flavonoids, tannins, alkaloids and amino acids. Large amounts of industrial waste are generated during the industrial processing of pomegranates, and 40 to 50% of the whole fruit is typically discarded 1. In this context, the present work was conducted to determine the chemical composition and bioactive properties of pomegranate leaves to support their potential utilisation as functional ingredients. Three extraction methodologies were evaluated: maceration, microwave and ultrasound-assisted extractions. The chemical composition of the different extracts was determined using HPLC-DAD-ESI/MS. The antioxidant potential was assessed using two cell-based assays: TBARS and CAA. Sulforhodamine B colourimetric assay was used to assess the antiproliferative capacity using several tumour cell lines and the primary culture of non-tumour cells (PLP2). The anti-inflammatory activity was measured through the extract's capacity to inhibit nitric oxide production. Finally, antimicrobial activity was evaluated using the microdilution method. The results showed that the main phenolic compounds found in the three extracts were gallic and caffeic acid derivatives, and flavonoids such as luteolin, apigenin, quercetin, and kaempferol derivatives. All the extracts exhibited capacity to inhibit tumor cell lines proliferation (GI50 of 19 - 76 μg/mL). Gastric adenocarcinoma (AGS) showed a higher sensitivity to all three leaf extraction methodologies. All three extracts presented lower IC50 values in the TBARS antioxidant assay (0.83 - 1.70 μg/mL) than the positive control Trolox (IC50 = 9.1 ± 0.3 μg/mL). The extracts presented a broad-spectrum antimicrobial inhibition with. K. pneumoniae showing the highest susceptibility to the extracts, (MIC values 0.6 mg/mL). These results suggest that pomegranate leaves can be sustainably exploited as a source of health-promoting biomolecules, to be used as functional ingredients in some biobased applications.pt_PT
dc.description.sponsorshipTo the Foundation for Science and Technology (FCT, Portugal) for financial support to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021); PhD grant of Filipa Mandim (SFRH/146614/2019) and L. Barros contract. Also, to FEDER for financial support through the project OliveBIOextract (NORTE-01-0247-FEDER-049865). This work is also supported by MICINN supporting the Ramón y Cajal grant for M.A. Prieto (RYC-2017-22891) and Juan de la Cierva Formation contract for T. Oludemi (FJC2019- 042549-I).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMarcelino, Sandra Andreia Gonçalves; Oludemi, Taofiq; Mandim, Filipa; Finimundy, Tiane C.; Prieto Lage, Miguel A.; Barros, Lillian (2022). How do extraction methodologies influence the biological properties of pomegranate leaves? In Livro de Resumos do 12º Encontro Nacional de Cromatografia & XIV WARPA. Aveiropt_PT
dc.identifier.urihttp://hdl.handle.net/10198/27904
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSociedade Portuguesa de Químicapt_PT
dc.relationLA/P/0007/2021pt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.relationRYC-2017-22891pt_PT
dc.relationIntegrated strategies for the development of a novel functional beverage with sustainable production systems
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPomegranate leavespt_PT
dc.subjectResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technologypt_PT
dc.titleHow do extraction methodologies influence the biological properties of pomegranate leaves?pt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardTitleIntegrated strategies for the development of a novel functional beverage with sustainable production systems
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F146614%2F2019/PT
oaire.citation.conferencePlaceAveiropt_PT
oaire.citation.endPage105pt_PT
oaire.citation.startPage105pt_PT
oaire.citation.titleLivro de Resumos do 12º Encontro Nacional de Cromatografia & XIV WARPApt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMarcelino
person.familyNameOludemi
person.familyNameMandim
person.familyNameFinimundy
person.familyNamePrieto
person.familyNameBarros
person.givenNameSandra Andreia
person.givenNameTaofiq
person.givenNameFilipa
person.givenNameTiane C.
person.givenNameMiguel A.
person.givenNameLillian
person.identifier107688
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person.identifier.ciencia-idE318-A5CD-6626
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person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0003-1490-9445
person.identifier.orcid0000-0003-2822-3532
person.identifier.orcid0000-0002-6494-2326
person.identifier.orcid0000-0003-3516-0795
person.identifier.orcid0000-0002-3513-0054
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridG-4516-2011
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id35937495700
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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