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Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies

2022Journal article Open access
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dc.contributor.authorLourenço-Lopes, Catarina
dc.contributor.authorFraga-Corral, Maria
dc.contributor.authorSoria-Lopez, Anton
dc.contributor.authorNúñez Estévez, Bernabé
dc.contributor.authorBarral Martínez, Marta
dc.contributor.authorSilva, Aurora
dc.contributor.authorLi, Ningyang
dc.contributor.authorLiu, Chao
dc.contributor.authorSimal-Gandara, Jesus
dc.contributor.authorPrieto Lage, Miguel A.
dc.date.accessioned2010-02-17T17:57:17Z
dc.date.available2010-02-17T17:57:17Z
dc.date.issued2022
dc.description.abstractBrown macroalgae are a potential source of natural pigments. Among them, Undaria pinnatifida is recognized for its high concentration of fucoxanthin (Fx), which is a pigment with a wide range of bioactivities. In this study, three independent parameters were optimized for conventional heat extraction (CHE) to maximize the recovery of Fx from Undaria pinnatifida. Optimal conditions (temperature = 45 °C, solvent = 70%, and time = 61 min) extracted 5.1 mg Fx/g dw. Later, the bioactivities of the Fx-rich extracts (antioxidant, antimicrobial, and neuroprotective) were assessed using in vitro and in silico approaches. In vitro assays indicated that Fx has a strong antioxidant capacity and even stronger antimicrobial activity against gram-positive bacteria. This data was supported in silico where Fx established a high binding affinity to DR, a Staphylococcus aureus protein, through aa ALA-8, LEU-21, and other alkane interactions. Finally, the in vitro enzymatic inhibition of AChE using Fx, was further supported using docking models that displayed Fx as having a high affinity for aa TYR72 and THR 75; therefore, the Fx extraction behavior explored in this work may reduce the costs associated with energy and solvent consumption. Moreover, this paper demonstrates the efficiency of CHE when recovering high amounts of Fx from Undaria pinnatifida. Furthermore, these findings can be applied in different industries.pt
dc.identifier.citationLourenço-Lopes, Catarina; Fraga-Corral, Maria; Soria-Lopez, Anton; Núñez Estévez, Bernabé; Barral-Martinez, Marta; Silva, Aurora; Li, Ningyang; Liu, Chao; Simal-Gandara, Jesus; Prieto, Miguel A. (2022). Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies. Antioxidants. EISSN 2076-3921. 11:7, p. 1-25pt
dc.identifier.issn2076-3921
dc.identifier.urihttp://hdl.handle.net/10198/1977
dc.language.isoengpt
dc.publisherMDPIpt
dc.subjectFucoxanthinpt
dc.subjectConventional heat extractionpt
dc.subjectUndaria pinnatifida
dc.subjectKinetics
dc.subjectAntioxidant activity
dc.subjectNeuroprotective activity
dc.subjectAntimicrobial activity
dc.subjectIn silico studies
dc.subjectDocking
dc.titleFucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studiespt
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleAntioxidants
person.familyNameFraga-Corral
person.familyNameNúñez Estévez
person.familyNamePrieto
person.givenNameMaria
person.givenNameBernabé
person.givenNameMiguel A.
person.identifier1054547
person.identifier107688
person.identifier.ciencia-id1616-98AD-4546
person.identifier.ciencia-id3214-F893-6AC2
person.identifier.ciencia-id221E-1E00-AB74
person.identifier.orcid0000-0002-5663-9239
person.identifier.orcid0000-0002-6435-7952
person.identifier.orcid0000-0002-3513-0054
person.identifier.ridG-7335-2017
person.identifier.ridG-4516-2011
person.identifier.scopus-author-id56346469200
person.identifier.scopus-author-id35937495700
rcaap.rightsopenAccesspt
rcaap.typearticlept
relation.isAuthorOfPublication446f0a90-be7c-4111-9949-42475fda0e6a
relation.isAuthorOfPublication3101a08b-d13b-4eec-aa19-8f87e7e99dcc
relation.isAuthorOfPublication13260615-fd28-4b8b-9dd4-8a8466007579
relation.isAuthorOfPublication.latestForDiscovery446f0a90-be7c-4111-9949-42475fda0e6a

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