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The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model

2021Conference poster Open access
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dc.contributor.authorJesus, T.M.
dc.contributor.authorSilva-Reis, Rita
dc.contributor.authorPires, Maria João
dc.contributor.authorLanzarin, Germano
dc.contributor.authorFélix, Luís
dc.contributor.authorVenâncio, Carlos
dc.contributor.authorCosta, Rui Miguel Gil
dc.contributor.authorBastos, Margarida M.S.M.
dc.contributor.authorMedeiros, Rui
dc.contributor.authorNogueira, António José M.
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorRosa, Eduardo
dc.contributor.authorOliveira, Paula A.
dc.date.accessioned2023-03-15T11:23:15Z
dc.date.available2023-03-15T11:23:15Z
dc.date.issued2021
dc.description.abstractInfection by Human Papillomavirus (HPV) is the main cause of cervical cancer, highlighting the importance of studying compounds that may reduce viral activity and its lesions/symptoms. The aromatic herb spearmint (Mentha spicata) (MS) has proven anti-tumor properties [1]. Thus, this study aimed to evaluate the effects of an hydroethanolic extract obtained from spearmint in HPV16-transgenic (HPV+/-) mice. The extract was obtained through maceration with ethanol/water (80:20, v/v), and the phenolic composition was determined through HPLC-DAD-ESI/MS. Thirty-three female mice (16 HPV-/- and 17 HPV+/-) were randomly divided into six groups: Group (G) I – HPV-/- w/o Mentha (n=5); G II – HPV-/- w/ Mentha (0.5 mg/ml; n=6); G III – HPV-/- w/ Mentha (0.55 mg/ml; n=5); G IV – HPV+/- w/o Mentha (n=6); G V – HPV+/- w/ Mentha (0.5 mg/ml; n=6); G VI – HPV+/- w/ Mentha (0.55 mg/ml; n=5). The spearmint extract was administered in the animal’s drinking water for 28 days. During the study, water and food intake as well the animals’ weights were recorded weekly. Afterwards, the animals were sacrificed, and their organs were collected for oxidative stress and genetic damage analysis. A total of thirteen compounds were identified in the hydroethanolic extract, being salvianolic acid B, rosmarinic acid and luteolin-7-O-glucuronide, the main compounds found. Moreover, the compounds revealed to be stable in the drinking water during the 5 tested days. Results show that HPV+/- (GIV, V and VI) have lower weight but higher water and food intake, as expected. Analysis of HPV+/- mice GIV showed a significant increase of superoxide dismutase activity when compared with GV (p=0.0029) and VI (p=0.0011). This suggests that group IV was subjected to a higher oxidative stress, as expected. These results could also mean that MS is responsible for a decrease in the oxidative stress known to be induced by HPV, which in turn decreases SOD, further the role of MS as an antioxidant. Regarding genetic damage, no statistically significant changes between groups were found in the comet and micronucleus assays, implying that spearmint has no influence on genotoxicity at the concentrations employed. There were no significant differences concerning basal DNA damage between WT and transgenic individuals as seen in other studies [2]. Further studies are required to clarify the antioxidant and antigenotoxic effects of spearmint.pt_PT
dc.description.sponsorshipInteract R&D project, NORTE-01-0145-FEDER-000017, in its ISAC research line, ERDF-NORTE 2020. The authors are also grateful to FCT, for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020); L. Barros thanks the national funding by FCT, P.I., through the institutional scientific employment program-contract.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJesus, T.M.; Silva-Reis, Rita; Pires, Maria João; Lanzarin, Germano; Félix, Luís; Venâncio, Carlos; Costa, Rui Miguel Gil; Bastos, Margarida M.S.M.; Medeiros, Rui; Nogueira, António José M.; Barros, Lillian; Ferreira, Isabel C.F.R.; Rosa, Eduardo; Oliveira, Paula A. (2021). The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model. In Lillian Barros; Bruno Melgar Castañeda; Carlos Seiti Hurtado Shiraishi (Eds.) 1st Natural products application: Health, Cosmetic and Food. Bragançapt_PT
dc.identifier.isbn978-972-745-286-6
dc.identifier.urihttp://hdl.handle.net/10198/27736
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstituto Politécnico de Bragançapt_PT
dc.relationNORTE-01-0145-FEDER- 000017pt_PT
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSpearmint (Mentha spicata)pt_PT
dc.subjectResearch Subject Categories::MEDICINE::Morphology, cell biology, pathologypt_PT
dc.titleThe anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse modelpt_PT
dc.typeconference poster
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.citation.conferencePlaceBragançapt_PT
oaire.citation.title1st Natural products application: Health, Cosmetic and Foodpt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameNogueira
person.familyNameBarros
person.familyNameFerreira
person.givenNameAntónio José M.
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id8A11-794E-36BD
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-8912-7355
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id55865568268
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication648209a2-eec9-4bec-975a-32673e41b95c
relation.isAuthorOfPublication3af07ffe-f914-48ba-a5d5-efcf70fdce01
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication.latestForDiscovery648209a2-eec9-4bec-975a-32673e41b95c
relation.isProjectOfPublication29718e93-4989-42bb-bcbc-4daff3870b25
relation.isProjectOfPublication.latestForDiscovery29718e93-4989-42bb-bcbc-4daff3870b25

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