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Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability

2024Artigo científico Acesso aberto
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dc.contributor.authorPascariu, Oana-Elena
dc.contributor.authorDias, L.G.
dc.contributor.authorIsrael-Roming, Florentina
dc.date.accessioned2024-10-08T11:23:19Z
dc.date.available2024-10-08T11:23:19Z
dc.date.issued2024
dc.description.abstractPhenolic compounds from elderberries (Sambucus nigra L.) have attracted attention due to their potential health benefits. This paper examines different extraction methods used to obtain phenolic compounds from these fruits and the stability of the extracts. Several extraction techniques (extraction with continuous agitation, ultrasound-assisted extraction, microwave-assisted extraction, maceration, and enzyme-assisted extraction) were tested and compared to evaluate the yield and the quality of the extracts. The stability of the extracts with various storage parameters (time and temperature) and processing conditions (concentration and lyophilization) was also investigated. The results showed that ultrasound-assisted extraction (UAE) provided the highest yield of total phenolic compounds (74.89 mg GAE/g), of which 71.23% were represented by total anthocyanins and 62.50% by monomeric anthocyanins, with total flavonoids of 8.11–9.41 mg RUE/g. The analysis of individual phenolic compounds reconfirms the efficiency of UAE, obtaining 0.42–0.09 mg/g gallic acid, 0.59–0.01 mg/g chlorogenic acid, 0.17–0.03 mg/g 4-coumaric acid, and 2.43–0.01 mg/g rutin. The optimization of the extraction conditions led to the conclusion that the best solvent is 45% ethanol (v/v), and the optimal parameters are 40 ◦C for 40 min. It was also found that the stability of the extracts can be high during long periods of time (even after 180 days). These findings contribute to the understanding of the optimization of extraction processes and storage conditions to obtain extracts rich in phenolic compounds from elderberries, with potential uses in pharmaceutical andb food applications.en
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPascariu, Oana-Elena; Dias, Luís Guimarães; Israel-Roming, Florentina (2024). Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability. Horticulturae. ISSN 2311-7524. 10:7, p. 1-20pt_PT
dc.identifier.doi10.3390/horticulturae10070743pt_PT
dc.identifier.issn2311-7524
dc.identifier.urihttp://hdl.handle.net/10198/30365
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSambucus nigrapt_PT
dc.subjectBioactive compoundspt_PT
dc.subjectExtractionpt_PT
dc.subjectPhenolic compoundspt_PT
dc.titleOptimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stabilitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue7pt_PT
oaire.citation.startPage743pt_PT
oaire.citation.titleHorticulturaept_PT
oaire.citation.volume10pt_PT
person.familyNameDias
person.givenNameLuís G.
person.identifier.ciencia-id2F11-9092-FAAF
person.identifier.orcid0000-0002-1210-4259
person.identifier.scopus-author-id23569169900
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationeac8c166-4056-4ed0-8d8d-7ecb2c4481a5
relation.isAuthorOfPublication.latestForDiscoveryeac8c166-4056-4ed0-8d8d-7ecb2c4481a5

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