Repository logo
 
Publication

Investigation of lamb as a source of shigatoxin-producing escherichia coli in Portugal

2019Conference object Open access
Simple item page
dc.contributor.authorPereira-Lima, Ana
dc.contributor.authorChoupina, Altino
dc.contributor.authorCadavez, Vasco
dc.contributor.authorGonzales-Barron, Ursula
dc.date.accessioned2019-09-26T08:48:30Z
dc.date.available2019-09-26T08:48:30Z
dc.date.issued2019
dc.description.abstractThe gastrointestinal tract of ruminants constitutes the main natural reservoir of shigatoxin-producing Escherichia coli (STEC), which is a serious pathogen that can be transmitted to humans through contaminated raw/undercooked meat products, raw milk and raw vegetables. However, the number of epidemiological studies investigating Portuguese sheep as a source of STEC strains is quite limited. Thus, the objective of this work was to assess the occurrence of STEC in apparently-healthy lambs from Portuguese breeds – Churra-Galega-Bragançana (CGB) and Bordaleira-Entre-Douro-e-Minho (BEDM), and determine the presence of major virulence genes in the isolates. Methodology: Six sheep farms located in Northeastern Portugal were visited, and recto-anal samples from a total of 50 CGB and 7 BEDM 4-month old lambs were taken, and enriched in 9-mL TSB with 20 mg/L novobiocin. After incubation at 37ºC for 24 h, samples were streaked onto SMAC agar with 0.05 mg/L cefixime and 2.5 mg/L potassium tellurite, and incubated at 37ºC for 24 h. Typical colonies were confirmed biochemically by indole, methyl-red and Voges-Proskauer tests, and serologically by O157 latex agglutination. Purified isolates were subjected to multiplex PCR to determine the genes encoding for shigatoxin (stx1, stx2), enterohemolysin (hlyA), and intimin (eae). Results: From the BEDM breed samples, no E. coli O157 was recovered (0/7), while only two shedders of sorbitol-negative E. coli were found in the CGB group (incidence 4.0%; 95% CI: 1.1 – 13.5%). PCR analysis of 10 isolates of sorbitol-negative E. coli coming from one positive lamb revealed the presence of two different strains, one coding for stx1 gene (8/10=80%) and the other coding for both stx1 and hlyA genes (3/10=30%). Sorbitol-negative colonies (3) isolated from one animal did not present any of the genes. Conclusion and Relevance: Although the overall prevalence of shigatoxin-producing E. coli in recto-anal contents from apparently-healthy lamb was low (1.75%; 95% CI: 0.09 – 10.63%), these findings are important for public health. Preventive measures are necessary to control the incidence of STEC infections in sheep and people. Good hygiene practices are necessary particularly in the slaughterhouse where contamination of lamb meat and environment with intestinal contents can be prevented. Provide five keywords separated by semicolon Sheep; recto-anal swab; PCR; virulencept_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPereira-Lima, Ana; Choupina, Altino; Cadavez, Vasco; Gonzales-Barron, Ursula (2019). Investigation of lamb as a source of shigatoxin-producing escherichia coli in Portugal. In Gonzales-Barron, Ursula; Cadavez, Vasco (Eds.) 11th International Conference on Predictive Modelling in Food: book of abstracts. Bragança: Instituto Politécnico de Bragança. ISBN 978-972-745-261-3pt_PT
dc.identifier.isbn978-972-745-261-3
dc.identifier.urihttp://hdl.handle.net/10198/19625
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstituto Politécnico de Bragançapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSheeppt_PT
dc.subjectRecto-anal swabpt_PT
dc.subjectPCRpt_PT
dc.subjectVirulencept_PT
dc.titleInvestigation of lamb as a source of shigatoxin-producing escherichia coli in Portugalpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceBragança, Portugalpt_PT
oaire.citation.endPage100pt_PT
oaire.citation.startPage100pt_PT
oaire.citation.title11th International Conference on Predictive Modelling in Foodpt_PT
oaire.citation.volume1pt_PT
person.familyNameChoupina
person.familyNameCadavez
person.familyNameGonzales-Barron
person.givenNameAltino
person.givenNameVasco
person.givenNameUrsula
person.identifier587972
person.identifierR-000-HDG
person.identifier.ciencia-id1A14-77FC-9656
person.identifier.ciencia-id441B-01AB-A12E
person.identifier.ciencia-id0813-C319-B62A
person.identifier.orcid0000-0002-3956-9398
person.identifier.orcid0000-0002-3077-7414
person.identifier.orcid0000-0002-8462-9775
person.identifier.ridL-5382-2014
person.identifier.ridA-3958-2010
person.identifier.scopus-author-id14051602500
person.identifier.scopus-author-id9039121900
person.identifier.scopus-author-id9435483700
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication314b895b-a5e2-4a92-9bc6-dd708c819be4
relation.isAuthorOfPublication57b410e9-f6b7-42ff-ab3d-b526278715eb
relation.isAuthorOfPublication17c6b98f-4fb5-41d3-839a-6f77ec70021a
relation.isAuthorOfPublication.latestForDiscovery17c6b98f-4fb5-41d3-839a-6f77ec70021a

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Investigation of lamb as a source of shigatoxin.pdf
Size:
528.45 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Resumos em Proceedings Não Indexados à WoS/Scopus