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Kale extract increases glutathione levels in V79 cells, but does not protect them against acute toxicity induced by hydrogen peroxide

2012Journal article Open access
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dc.contributor.authorFernandes, Fátima
dc.contributor.authorSousa, Carla
dc.contributor.authorFerreres, Frederico
dc.contributor.authorValentão, Patrícia
dc.contributor.authorRemião, Fernando
dc.contributor.authorPereira, J.A.
dc.contributor.authorAndrade, Paula B.
dc.date.accessioned2016-12-21T10:02:22Z
dc.date.available2016-12-21T10:02:22Z
dc.date.issued2012
dc.description.abstractThis study aims to evaluate the antioxidant potential of extracts of Brassica oleracea L. var. acephala DC. (kale) and several materials of Pieris brassicae L., a common pest of Brassica cultures using a cellular model with hamster lung fibroblast (V79 cells) under quiescent conditions and subjected to H₂O₂ induced oxidative stress. Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and glutathione was determined by the 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB)-oxidized glutathione (GSSG) reductase recycling assay. The phenolic composition of the extracts was also established by HPLC-DAD. They presented acylated and non acylated flavonoid glycosides, some of them sulfated, and hydroxycinnamic acyl gentiobiosides. All extracts were cytotoxic by themselves at high concentrations and failed to protect V79 cells against H₂O₂ acute toxicity. No relationship between phenolic composition and cytotoxicity of the extracts was found. Rather, a significant increase in glutathione was observed in cells exposed to kale extract, which contained the highest amount and variety of flavonoids. It can be concluded that although flavonoids-rich extracts have the ability to increase cellular antioxidant defenses, the use of extracts of kale and P. brassicae materials by pharmaceutical or food industries, may constitute an insult to health, especially to debilitated individuals, if high doses are consumed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFernandes, Fátima; Sousa, Carla; Ferreres, F.; Valentão, Patrícia; Remião, Fernando; Pereira, José A.; Andrade, Paula B. (2012) – Kale extract increases glutathione levels in V79 cells, but does not protect them against acute toxicity induced by hydrogen peroxide. Molecules. ISSN 1420-3049. 17:5, p. 5269-5288pt_PT
dc.identifier.doi10.3390/molecules17055269pt_PT
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10198/13629
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationStrategic Project - LA 6 - 2011-2012
dc.relationPIERIS BRASSICAE FED WITH BRASSICA OLERACEA VAR. COSTATA AND BRASSICA OLERACEA VAR. ACEPHALA AS SOURCE OF BIOACTIVE COMPOUNDS
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBrassica oleracea var. acephalapt_PT
dc.subjectPieris brassicaept_PT
dc.subjectPhenolic compoundspt_PT
dc.subjectV79 cellspt_PT
dc.subjectOxidative stresspt_PT
dc.subjectGlutathione statuspt_PT
dc.titleKale extract increases glutathione levels in V79 cells, but does not protect them against acute toxicity induced by hydrogen peroxidept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 6 - 2011-2012
oaire.awardTitlePIERIS BRASSICAE FED WITH BRASSICA OLERACEA VAR. COSTATA AND BRASSICA OLERACEA VAR. ACEPHALA AS SOURCE OF BIOACTIVE COMPOUNDS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FEQB%2FLA0006%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F37963%2F2007/PT
oaire.citation.endPage5288pt_PT
oaire.citation.issue12pt_PT
oaire.citation.startPage5269pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume17pt_PT
oaire.fundingStream6820 - DCRRNI ID
person.familyNamePereira
person.givenNameJosé Alberto
person.identifier.ciencia-id611F-80B2-A7C1
person.identifier.orcid0000-0002-2260-0600
person.identifier.ridL-6798-2014
person.identifier.scopus-author-id57204366348
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication7932162e-a2da-4913-b00d-17babbe51857
relation.isAuthorOfPublication.latestForDiscovery7932162e-a2da-4913-b00d-17babbe51857
relation.isProjectOfPublicationc40e3666-1466-44a3-b595-b3c38d27fa0b
relation.isProjectOfPublicatione6c5a113-78fb-4279-ba0c-f98bcdcedd12
relation.isProjectOfPublication.latestForDiscoveryc40e3666-1466-44a3-b595-b3c38d27fa0b

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