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Antibacterial efficacy of Enterococcus microencapsulated bacteriocin on Listeria monocytogenes, Listeria innocua and Listeria ivanovi

2023Journal article Open access
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dc.contributor.authorRamalho, Regiane
dc.contributor.authorSouza, Nathália A.A. de
dc.contributor.authorMoreira, Thaysa Fernandes Moya
dc.contributor.authorOliveira, Anielle
dc.contributor.authorPerini, Hugo Felix
dc.contributor.authorFurlaneto, Márcia Cristina
dc.contributor.authorLeimann, Fernanda Vitória
dc.contributor.authorFurlaneto-Maia, Luciana
dc.date.accessioned2024-01-05T12:16:40Z
dc.date.available2024-01-05T12:16:40Z
dc.date.issued2023
dc.description.abstractThis study focused on the microencapsulation of enterocin from Enterococcus durans (E. durans MF5) in whey powder (WP) using a spray-drying technique followed by the evaluation of how complexation can preserve the enterocin structure and antimicrobial activity against foodborne pathogens. Crude enterocin samples (1 and 5%) were microencapsulated in 10% WP. The antimicrobial activity of unencapsulated (crude) enterocin and microencapsulated enterocin was tested against the target bacteria Salmonella Typhimurium, Escherichia coli, Listeria monocytogenes, Listeria innocua, and Listeria ivanovi. The microencapsulation yields were 31.66% and 34.16% for concentrations of 1 and 5% enterocin, respectively. There was no significant difference between these concentrations. Microencapsulated enterocin was efficient for up to 12 h of cocultivation with Listeria sp., and the concentration required to inhibit the growth of target bacteria presented values of 6400 AU/ mL (arbitrary unit). Microencapsulated enterocin demonstrated enhanced efficacy against Listeria species and E. coli when compared with crude enterocin (p < 0.05). Fourier transform-infrared spectroscopy and differential scanning calorimetry results confirmed the presence of enterocin in the microparticles. Scanning electron microscopy showed cell damage of the target bacteria. The results showed that complexation with WP preserved enterocin antimicrobial activity during spray-drying, indicating its potential use as a food preservative.pt_PT
dc.description.sponsorshipThis work was supported by Fundação Araucária/Governo do Paraná—Brazil, UTFPR-Londrina Campus and State University of Londrina-Paraná. The authors thank the “Central Analítica Multiusuário da UTFPR Campo Mourão (CAMulti-CM)¨ and “Laboratório Multiusuário da UTFPR Campus Londrina (LabMulti-LD)¨ for the analyses. Fernanda V. Leimann (process 039/2019) thanks to Fundação Araucária (CP 15/2017- Programa de Bolsas de Produtividade em Pesquisa e Desenvolvimento Tecnológico) and to CNPq (process number 421541/2018-0, Chamada Universal MCTIC/CNPq n.° 28/2018).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRamalho, Regiane; Souza, Nathália A.A. de; Moreira, Thaysa Fernandes Moya; Oliveira, Anielle; Perini, Hugo Felix; Furlaneto, Márcia Cristina; Leimann, Fernanda Vitória; Furlaneto-Maia, Luciana (2023). Antibacterial efficacy of Enterococcus microencapsulated bacteriocin on Listeria monocytogenes, Listeria innocua and Listeria ivanovi. Journal of Food Science and Technology. ISSN 0022-1155. 60:1, p. 262-271pt_PT
dc.identifier.doi10.1007/s13197-022-05611-0pt_PT
dc.identifier.eissn0975-8402
dc.identifier.issn0022-1155
dc.identifier.urihttp://hdl.handle.net/10198/29106
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectWhey proteinpt_PT
dc.subjectSpray dryer processpt_PT
dc.subjectSalmonella Typhimuriumpt_PT
dc.subjectEscherichia colipt_PT
dc.titleAntibacterial efficacy of Enterococcus microencapsulated bacteriocin on Listeria monocytogenes, Listeria innocua and Listeria ivanovipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage271pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage262pt_PT
oaire.citation.titleJournal of Food Science and Technologypt_PT
oaire.citation.volume60pt_PT
person.familyNameLeimann
person.givenNameFernanda Vitória
person.identifier.ciencia-id8F10-A04D-FC0D
person.identifier.orcid0000-0001-6230-9597
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb1102a0b-38dc-4caa-baed-ad52ccd9b230
relation.isAuthorOfPublication.latestForDiscoveryb1102a0b-38dc-4caa-baed-ad52ccd9b230

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