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Advisor(s)
Abstract(s)
This study focused on the microencapsulation
of enterocin from Enterococcus durans (E. durans MF5) in
whey powder (WP) using a spray-drying technique followed
by the evaluation of how complexation can preserve the
enterocin structure and antimicrobial activity against foodborne
pathogens. Crude enterocin samples (1 and 5%) were
microencapsulated in 10% WP. The antimicrobial activity
of unencapsulated (crude) enterocin and microencapsulated
enterocin was tested against the target bacteria Salmonella
Typhimurium, Escherichia coli, Listeria monocytogenes,
Listeria innocua, and Listeria ivanovi. The microencapsulation
yields were 31.66% and 34.16% for concentrations of
1 and 5% enterocin, respectively. There was no significant
difference between these concentrations. Microencapsulated
enterocin was efficient for up to 12 h of cocultivation
with Listeria sp., and the concentration required to inhibit
the growth of target bacteria presented values of 6400 AU/
mL (arbitrary unit). Microencapsulated enterocin demonstrated
enhanced efficacy against Listeria species and E.
coli when compared with crude enterocin (p < 0.05). Fourier transform-infrared spectroscopy and differential scanning
calorimetry results confirmed the presence of enterocin in
the microparticles. Scanning electron microscopy showed
cell damage of the target bacteria. The results showed that
complexation with WP preserved enterocin antimicrobial
activity during spray-drying, indicating its potential use as
a food preservative.
Description
Keywords
Whey protein Spray dryer process Salmonella Typhimurium Escherichia coli
Citation
Ramalho, Regiane; Souza, Nathália A.A. de; Moreira, Thaysa Fernandes Moya; Oliveira, Anielle; Perini, Hugo Felix; Furlaneto, Márcia Cristina; Leimann, Fernanda Vitória; Furlaneto-Maia, Luciana (2023). Antibacterial efficacy of Enterococcus microencapsulated bacteriocin on Listeria monocytogenes, Listeria innocua and Listeria ivanovi. Journal of Food Science and Technology. ISSN 0022-1155. 60:1, p. 262-271
Publisher
Springer Nature