Logo do repositório
 
Publicação

Genomic DNA extraction from honey bee (Apis mellifera) queen spermathecal content

2025Artigo científico Acesso aberto
Ver registo simples
datacite.subject.fosCiências Agrárias::Agricultura, Silvicultura e Pescas
datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg15:Proteger a Vida Terrestre
dc.contributor.authorYadró, Carlos A.
dc.contributor.authorLopes, Ana Rita
dc.contributor.authorHenriques, Dora
dc.contributor.authorMusin, Eduard
dc.contributor.authorWegener, Jakob
dc.contributor.authorPinto, M. Alice
dc.date.accessioned2025-03-10T11:31:53Z
dc.date.available2025-03-10T11:31:53Z
dc.date.issued2025
dc.description.abstractGenetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing. The four kits were tested with different adjustments in the lysis incubation time, use of RNA-carrier, elution conditions and number of re-elutions. Only the use of QIAamp DNA Microkit with 3 h of lysis incubation, the addition of RNA-carrier and multiple re-elutions produced a DNA concentration over the required threshold.pt_PT
dc.description.sponsorshipContributions of Jakob Wegener and Eduard Musin were supported by funds of the Federal Ministry of Food and Agriculture based on a decision of the Parliament of the Federal Republic of Germany via the Federal Office for Agriculture and Food, grant number 2818BM040. Dora Henriques was supported by BEEHAPPY (POCI-01-0145- FEDER-029871; FCT and COMPETE/QREN/EU). Ana R. Lopes and Carlos A. Yadró were supported by Fundação para a Ciência e a Tecnologia (FCT) grants SFRH/BD/143627/2019 and 2021.06948.BD, respectively. FCT provided financial support by national funds (FCT/MCTES) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationYadró, Carlos A.; Lopes, Ana Rita; Henriques, Dora; Musin, Eduard; Wegener, Jakob; Pinto, M. Alice (2023). Genomic DNA extraction from honey bee (Apis mellifera) queen spermathecal content. Journal of Apicultural Research. ISSN 0021-8839. 64:1, p. 198-201
dc.identifier.doi10.1080/00218839.2023.2262871pt_PT
dc.identifier.issn0021-8839
dc.identifier.urihttp://hdl.handle.net/10198/34339
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor and Francis
dc.relationLA/P/0007/2021pt_PT
dc.relationHoneybees Apis mellifera L. in the Azores: developing innovative molecular tools and uncovering host-pathogen interactions in a pre- and post-Varroa destructor invasion
dc.relationMediterranean honeybees Apis mellifera: a genome-wide approach to dissect neutral and adaptive patterns of genetic diversity
dc.relationMountain Research Center - UIDB/00690/2020
dc.relationMountain Research Center - UIDP/00690/2020
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHoney beept_PT
dc.subjectSpermathecapt_PT
dc.subjectGenomic DNA isolationpt_PT
dc.subjectDNA extractionpt_PT
dc.titleGenomic DNA extraction from honey bee (Apis mellifera) queen spermathecal contentpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberSFRH/BD/143627/2019
oaire.awardNumber2021.06948.BD
oaire.awardNumberUIDB/00690/2020
oaire.awardNumberUIDP/00690/2020
oaire.awardTitleHoneybees Apis mellifera L. in the Azores: developing innovative molecular tools and uncovering host-pathogen interactions in a pre- and post-Varroa destructor invasion
oaire.awardTitleMediterranean honeybees Apis mellifera: a genome-wide approach to dissect neutral and adaptive patterns of genetic diversity
oaire.awardTitleMountain Research Center - UIDB/00690/2020
oaire.awardTitleMountain Research Center - UIDP/00690/2020
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F143627%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2021.06948.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.citation.endPage201
oaire.citation.issue1
oaire.citation.startPage198
oaire.citation.titleJournal of Apicultural Researchpt_PT
oaire.citation.volume64
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameHenriques
person.familyNamePinto
person.givenNameDora
person.givenNameM. Alice
person.identifier.ciencia-id291F-986F-07DA
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.orcid0000-0001-7530-682X
person.identifier.orcid0000-0001-9663-8399
person.identifier.scopus-author-id55761737300
person.identifier.scopus-author-id8085507800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd2abd09f-a90c-4cfb-9a60-7fc32f56184d
relation.isAuthorOfPublication0667fe04-7078-483d-9198-56d167b19bc5
relation.isAuthorOfPublication.latestForDiscoveryd2abd09f-a90c-4cfb-9a60-7fc32f56184d
relation.isProjectOfPublicationab2b064a-cba6-4d66-ad3e-470553d74da0
relation.isProjectOfPublication62fd7554-7bc1-478c-9aa5-7642aa6761b0
relation.isProjectOfPublication29718e93-4989-42bb-bcbc-4daff3870b25
relation.isProjectOfPublication0aac8939-28c2-46f4-ab6b-439dba7f9942
relation.isProjectOfPublication.latestForDiscovery0aac8939-28c2-46f4-ab6b-439dba7f9942

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Genomic-DNA-extraction-from-honey-bee-Apis.pdf
Tamanho:
1.44 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.75 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CIMO - Artigos em Revistas Indexados à WoS/Scopus